Antiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated HOTAIR/Akt signaling pathway: In vitro

The overexpression of lncRNA HOTAIR can result in cancer progression through upregulation of the Akt signaling pathway. We aimed to evaluate the anticancer effects of levan polysaccharide from Erwinia herbicola on colorectal cancer cells (HT-29) and explore the role of the ROS-mediated HOTAIR/Akt si...

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Autores principales:	Tiancheng Zhan, Qian Bai, Zheng Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Levan polysaccharide Colorectal cancer Akt lncRNA HOTAIR Signaling Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/7f19d61ebb1e4350bd2dfc042e594bdb
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spelling	oai:doaj.org-article:7f19d61ebb1e4350bd2dfc042e594bdb2021-11-20T04:57:45ZAntiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated HOTAIR/Akt signaling pathway: In vitro1878-535210.1016/j.arabjc.2021.103389https://doaj.org/article/7f19d61ebb1e4350bd2dfc042e594bdb2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1878535221004044https://doaj.org/toc/1878-5352The overexpression of lncRNA HOTAIR can result in cancer progression through upregulation of the Akt signaling pathway. We aimed to evaluate the anticancer effects of levan polysaccharide from Erwinia herbicola on colorectal cancer cells (HT-29) and explore the role of the ROS-mediated HOTAIR/Akt signaling pathway. The HT-29 cancer cells were treated with levan either alone or along with N-acetyl-L-cysteine (NAC), as a potential antioxidant for 24 hrs and different assays including MTT, LDH, ROS, apoptosis, SOD activity, CAT activity, caspase-3 activity, qRT-PCR, and western blot analysis were performed. It was shown that levan treatment induces apparent reduction in cell viability, SOD and CAT activity, HOTAIR expression, the ratio of pAkt protein level and a significant increase in the ROS level, apoptosis induction, and caspase-3 activity, which were effectively suppressed by NAC co-treatment. These data indicated the effective antiproliferative effect of levan on colorectal cancer cells, in which downregulation of ROS-mediated HOTAIR/Akt plays an important role.Tiancheng ZhanQian BaiZheng ZhaoElsevierarticleLevan polysaccharideColorectal cancerAktlncRNAHOTAIRSignalingChemistryQD1-999ENArabian Journal of Chemistry, Vol 14, Iss 12, Pp 103389- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Levan polysaccharide Colorectal cancer Akt lncRNA HOTAIR Signaling Chemistry QD1-999
spellingShingle	Levan polysaccharide Colorectal cancer Akt lncRNA HOTAIR Signaling Chemistry QD1-999 Tiancheng Zhan Qian Bai Zheng Zhao Antiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated HOTAIR/Akt signaling pathway: In vitro
description	The overexpression of lncRNA HOTAIR can result in cancer progression through upregulation of the Akt signaling pathway. We aimed to evaluate the anticancer effects of levan polysaccharide from Erwinia herbicola on colorectal cancer cells (HT-29) and explore the role of the ROS-mediated HOTAIR/Akt signaling pathway. The HT-29 cancer cells were treated with levan either alone or along with N-acetyl-L-cysteine (NAC), as a potential antioxidant for 24 hrs and different assays including MTT, LDH, ROS, apoptosis, SOD activity, CAT activity, caspase-3 activity, qRT-PCR, and western blot analysis were performed. It was shown that levan treatment induces apparent reduction in cell viability, SOD and CAT activity, HOTAIR expression, the ratio of pAkt protein level and a significant increase in the ROS level, apoptosis induction, and caspase-3 activity, which were effectively suppressed by NAC co-treatment. These data indicated the effective antiproliferative effect of levan on colorectal cancer cells, in which downregulation of ROS-mediated HOTAIR/Akt plays an important role.
format	article
author	Tiancheng Zhan Qian Bai Zheng Zhao
author_facet	Tiancheng Zhan Qian Bai Zheng Zhao
author_sort	Tiancheng Zhan
title	Antiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated HOTAIR/Akt signaling pathway: In vitro
title_short	Antiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated HOTAIR/Akt signaling pathway: In vitro
title_full	Antiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated HOTAIR/Akt signaling pathway: In vitro
title_fullStr	Antiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated HOTAIR/Akt signaling pathway: In vitro
title_full_unstemmed	Antiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated HOTAIR/Akt signaling pathway: In vitro
title_sort	antiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated hotair/akt signaling pathway: in vitro
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7f19d61ebb1e4350bd2dfc042e594bdb
work_keys_str_mv	AT tianchengzhan antiproliferativeeffectsoflevanpolysaccharideagainstcolorectalcancercellsmediatedthroughoxidativestressstimulatedhotairaktsignalingpathwayinvitro AT qianbai antiproliferativeeffectsoflevanpolysaccharideagainstcolorectalcancercellsmediatedthroughoxidativestressstimulatedhotairaktsignalingpathwayinvitro AT zhengzhao antiproliferativeeffectsoflevanpolysaccharideagainstcolorectalcancercellsmediatedthroughoxidativestressstimulatedhotairaktsignalingpathwayinvitro
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Antiproliferative effects of levan polysaccharide against colorectal cancer cells mediated through oxidative stress-stimulated HOTAIR/Akt signaling pathway: In vitro

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