Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of <i>Theileria haneyi</i> in Egypt
Equine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites <i>Theileria equi</i>, <i>Babesia caballi</i>, and the recently identified species <i>T. haneyi</i>. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of <...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/7f2096120bc4460ea58148e2efe9ecb0 |
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| Sumario: | Equine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites <i>Theileria equi</i>, <i>Babesia caballi</i>, and the recently identified species <i>T. haneyi</i>. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of <i>T. equi</i> and <i>B. caballi</i> for the simultaneous detection of EP in Egyptian equids and examined the presence of <i>T. haneyi</i> infections in Egypt. Blood samples from 155 equids (79 horses and 76 donkeys) collected from different governorates of Egypt were examined by mPCR and PCR targeting <i>T. hayeni</i>. The mPCR method revealed a prevalence of <i>T. equi</i> of 20.3% in horses and of 13.1% in donkeys and a prevalence of <i>B</i>. <i>caballi</i> of 1.2% in horses. <i>B</i>. <i>caballi</i> was not detected in donkeys in the current study. The mPCR method also detected coinfections with both species (2.5% and 1.3% in horses and donkeys, respectively). Additionally, we report the presence of <i>T. haneyi</i> in Egypt for the first time in 53.1% of the horse and 38.1% of the donkey tested samples. Coinfection with <i>T. haneyi</i> and <i>T. equi</i> was found in 13.5% of the samples, while infection with the three EP species was found in 1.9% of the samples. |
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