Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of Theileria haneyi in Egypt

Equine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites Theileria equi, Babesia caballi, and the recently identified species T. haneyi. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of <...

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Autores principales:	Bassma S. M. Elsawy, Ahmed M. Nassar, Heba F. Alzan, Raksha V. Bhoora, Sezayi Ozubek, Mona S. Mahmoud, Omnia M. Kandil, Olfat A. Mahdy
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	equids <i>Babesia caballi</i> <i>Theileria equi</i> <i>Theileria haneyi</i> multiplex PCR cPCR Medicine R
Acceso en línea:	https://doaj.org/article/7f2096120bc4460ea58148e2efe9ecb0
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spelling	oai:doaj.org-article:7f2096120bc4460ea58148e2efe9ecb02021-11-25T18:38:05ZRapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of <i>Theileria haneyi</i> in Egypt10.3390/pathogens101114142076-0817https://doaj.org/article/7f2096120bc4460ea58148e2efe9ecb02021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1414https://doaj.org/toc/2076-0817Equine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites <i>Theileria equi</i>, <i>Babesia caballi</i>, and the recently identified species <i>T. haneyi</i>. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of <i>T. equi</i> and <i>B. caballi</i> for the simultaneous detection of EP in Egyptian equids and examined the presence of <i>T. haneyi</i> infections in Egypt. Blood samples from 155 equids (79 horses and 76 donkeys) collected from different governorates of Egypt were examined by mPCR and PCR targeting <i>T. hayeni</i>. The mPCR method revealed a prevalence of <i>T. equi</i> of 20.3% in horses and of 13.1% in donkeys and a prevalence of <i>B</i>. <i>caballi</i> of 1.2% in horses. <i>B</i>. <i>caballi</i> was not detected in donkeys in the current study. The mPCR method also detected coinfections with both species (2.5% and 1.3% in horses and donkeys, respectively). Additionally, we report the presence of <i>T. haneyi</i> in Egypt for the first time in 53.1% of the horse and 38.1% of the donkey tested samples. Coinfection with <i>T. haneyi</i> and <i>T. equi</i> was found in 13.5% of the samples, while infection with the three EP species was found in 1.9% of the samples.Bassma S. M. ElsawyAhmed M. NassarHeba F. AlzanRaksha V. BhooraSezayi OzubekMona S. MahmoudOmnia M. KandilOlfat A. MahdyMDPI AGarticleequids<i>Babesia caballi</i><i>Theileria equi</i><i>Theileria haneyi</i>multiplex PCRcPCRMedicineRENPathogens, Vol 10, Iss 1414, p 1414 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	equids <i>Babesia caballi</i> <i>Theileria equi</i> <i>Theileria haneyi</i> multiplex PCR cPCR Medicine R
spellingShingle	equids <i>Babesia caballi</i> <i>Theileria equi</i> <i>Theileria haneyi</i> multiplex PCR cPCR Medicine R Bassma S. M. Elsawy Ahmed M. Nassar Heba F. Alzan Raksha V. Bhoora Sezayi Ozubek Mona S. Mahmoud Omnia M. Kandil Olfat A. Mahdy Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of <i>Theileria haneyi</i> in Egypt
description	Equine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites <i>Theileria equi</i>, <i>Babesia caballi</i>, and the recently identified species <i>T. haneyi</i>. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of <i>T. equi</i> and <i>B. caballi</i> for the simultaneous detection of EP in Egyptian equids and examined the presence of <i>T. haneyi</i> infections in Egypt. Blood samples from 155 equids (79 horses and 76 donkeys) collected from different governorates of Egypt were examined by mPCR and PCR targeting <i>T. hayeni</i>. The mPCR method revealed a prevalence of <i>T. equi</i> of 20.3% in horses and of 13.1% in donkeys and a prevalence of <i>B</i>. <i>caballi</i> of 1.2% in horses. <i>B</i>. <i>caballi</i> was not detected in donkeys in the current study. The mPCR method also detected coinfections with both species (2.5% and 1.3% in horses and donkeys, respectively). Additionally, we report the presence of <i>T. haneyi</i> in Egypt for the first time in 53.1% of the horse and 38.1% of the donkey tested samples. Coinfection with <i>T. haneyi</i> and <i>T. equi</i> was found in 13.5% of the samples, while infection with the three EP species was found in 1.9% of the samples.
format	article
author	Bassma S. M. Elsawy Ahmed M. Nassar Heba F. Alzan Raksha V. Bhoora Sezayi Ozubek Mona S. Mahmoud Omnia M. Kandil Olfat A. Mahdy
author_facet	Bassma S. M. Elsawy Ahmed M. Nassar Heba F. Alzan Raksha V. Bhoora Sezayi Ozubek Mona S. Mahmoud Omnia M. Kandil Olfat A. Mahdy
author_sort	Bassma S. M. Elsawy
title	Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of <i>Theileria haneyi</i> in Egypt
title_short	Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of <i>Theileria haneyi</i> in Egypt
title_full	Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of <i>Theileria haneyi</i> in Egypt
title_fullStr	Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of <i>Theileria haneyi</i> in Egypt
title_full_unstemmed	Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of <i>Theileria haneyi</i> in Egypt
title_sort	rapid detection of equine piroplasms using multiplex pcr and first genetic characterization of <i>theileria haneyi</i> in egypt
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7f2096120bc4460ea58148e2efe9ecb0
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Rapid Detection of Equine Piroplasms Using Multiplex PCR and First Genetic Characterization of <i>Theileria haneyi</i> in Egypt

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