Effects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.

Trans fatty acid consumption in the human diet can cause adverse health effects, such as cardiovascular disease, which is associated with higher total cholesterol, a higher low density lipoprotein-cholesterol level and a decreased high density lipoprotein-cholesterol level. The aim of the study was...

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Autores principales:	Lone Vendel Nielsen, Toke P Krogager, Clifford Young, Carla Ferreri, Chryssostomos Chatgilialoglu, Ole Nørregaard Jensen, Jan J Enghild
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f21b5d355024acaa3d1715ace2c06f1
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spelling	oai:doaj.org-article:7f21b5d355024acaa3d1715ace2c06f12021-11-18T08:55:21ZEffects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.1932-620310.1371/journal.pone.0074283https://doaj.org/article/7f21b5d355024acaa3d1715ace2c06f12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24058537/?tool=EBIhttps://doaj.org/toc/1932-6203Trans fatty acid consumption in the human diet can cause adverse health effects, such as cardiovascular disease, which is associated with higher total cholesterol, a higher low density lipoprotein-cholesterol level and a decreased high density lipoprotein-cholesterol level. The aim of the study was to elucidate the hepatic response to the most abundant trans fatty acid in the human diet, elaidic acid, to help explain clinical findings on the relationship between trans fatty acids and cardiovascular disease. The human HepG2 cell line was used as a model to investigate the hepatic response to elaidic acid in a combined proteomic, transcriptomic and lipidomic approach. We found many of the proteins responsible for cholesterol synthesis up-regulated together with several proteins involved in the esterification and hepatic import/export of cholesterol. Furthermore, a profound remodeling of the cellular membrane occurred at the phospholipid level. Our findings contribute to the explanation on how trans fatty acids from the diet can cause modifications in plasma cholesterol levels by inducing abundance changes in several hepatic proteins and the hepatic membrane composition.Lone Vendel NielsenToke P KrogagerClifford YoungCarla FerreriChryssostomos ChatgilialogluOle Nørregaard JensenJan J EnghildPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74283 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lone Vendel Nielsen Toke P Krogager Clifford Young Carla Ferreri Chryssostomos Chatgilialoglu Ole Nørregaard Jensen Jan J Enghild Effects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.
description	Trans fatty acid consumption in the human diet can cause adverse health effects, such as cardiovascular disease, which is associated with higher total cholesterol, a higher low density lipoprotein-cholesterol level and a decreased high density lipoprotein-cholesterol level. The aim of the study was to elucidate the hepatic response to the most abundant trans fatty acid in the human diet, elaidic acid, to help explain clinical findings on the relationship between trans fatty acids and cardiovascular disease. The human HepG2 cell line was used as a model to investigate the hepatic response to elaidic acid in a combined proteomic, transcriptomic and lipidomic approach. We found many of the proteins responsible for cholesterol synthesis up-regulated together with several proteins involved in the esterification and hepatic import/export of cholesterol. Furthermore, a profound remodeling of the cellular membrane occurred at the phospholipid level. Our findings contribute to the explanation on how trans fatty acids from the diet can cause modifications in plasma cholesterol levels by inducing abundance changes in several hepatic proteins and the hepatic membrane composition.
format	article
author	Lone Vendel Nielsen Toke P Krogager Clifford Young Carla Ferreri Chryssostomos Chatgilialoglu Ole Nørregaard Jensen Jan J Enghild
author_facet	Lone Vendel Nielsen Toke P Krogager Clifford Young Carla Ferreri Chryssostomos Chatgilialoglu Ole Nørregaard Jensen Jan J Enghild
author_sort	Lone Vendel Nielsen
title	Effects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.
title_short	Effects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.
title_full	Effects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.
title_fullStr	Effects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.
title_full_unstemmed	Effects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.
title_sort	effects of elaidic acid on lipid metabolism in hepg2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7f21b5d355024acaa3d1715ace2c06f1
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Effects of elaidic acid on lipid metabolism in HepG2 cells, investigated by an integrated approach of lipidomics, transcriptomics and proteomics.

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