Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro

Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro

Giuseppe Maria de Peppo,1–3 Hossein Agheli,2,3 Camilla Karlsson,2,3 Karin Ekström,2,3 Helena Brisby,3,4 Maria Lennerås,2,3 Stefan Gustafsson,3,5 Peter Sjövall,3,5,6 Anna Johansson,2,3 Eva Olsson,3,5 Jukka Lausmaa,3,6 Peter Thomsen,2,3 Sarunas...

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Autores principales: de Peppo GM, Agheli H, Karlsson C, Ekström K, Brisby H, Lennerås M, Gustafsson S, Sjövall P, Johansson A, Olsson E, Lausmaa J, Thomsen P, Petronis S
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2014
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/7f2270fee41d4670ba03e0671ef9a450
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spelling oai:doaj.org-article:7f2270fee41d4670ba03e0671ef9a4502021-12-02T00:37:19ZOsteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro1178-2013https://doaj.org/article/7f2270fee41d4670ba03e0671ef9a4502014-05-01T00:00:00Zhttp://www.dovepress.com/osteogenic-response-of-human-mesenchymal-stem-cells-to-well-defined-na-a16945https://doaj.org/toc/1178-2013 Giuseppe Maria de Peppo,1–3 Hossein Agheli,2,3 Camilla Karlsson,2,3 Karin Ekström,2,3 Helena Brisby,3,4 Maria Lennerås,2,3 Stefan Gustafsson,3,5 Peter Sjövall,3,5,6 Anna Johansson,2,3 Eva Olsson,3,5 Jukka Lausmaa,3,6 Peter Thomsen,2,3 Sarunas Petronis3,6 1The New York Stem Cell Foundation Research Institute, New York, NY, USA; 2Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, 3BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, 4Department of Orthopaedics, Sahlgrenska Academy, University of Gothenburg, 5Applied Physics, Chalmers University of Technology, Göteborg, Sweden; 6Chemistry, Materials and Surfaces, SP Technical Research Institute of Sweden, Borås, Sweden Background: Patterning medical devices at the nanoscale level enables the manipulation of cell behavior and tissue regeneration, with topographic features recognized as playing a significant role in the osseointegration of implantable devices. Methods: In this study, we assessed the ability of titanium-coated hemisphere-like topographic nanostructures of different sizes (approximately 50, 100, and 200 nm) to influence the morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs). Results: We found that the proliferation and osteogenic differentiation of hMSCs was influenced by the size of the underlying structures, suggesting that size variations in topographic features at the nanoscale level, independently of chemistry, can be exploited to control hMSC behavior in a size-dependent fashion. Conclusion: Our studies demonstrate that colloidal lithography, in combination with coating technologies, can be exploited to investigate the cell response to well defined nanoscale topography and to develop next-generation surfaces that guide tissue regeneration and promote implant integration. Keywords: colloidal lithography, nanotopography, human mesenchymal stem cells, cell proliferation, osteogenic differentiation, mineralization, implantable materialsde Peppo GMAgheli HKarlsson CEkström KBrisby HLennerås MGustafsson SSjövall PJohansson AOlsson ELausmaa JThomsen PPetronis SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2499-2515 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
de Peppo GM
Agheli H
Karlsson C
Ekström K
Brisby H
Lennerås M
Gustafsson S
Sjövall P
Johansson A
Olsson E
Lausmaa J
Thomsen P
Petronis S
Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
description Giuseppe Maria de Peppo,1–3 Hossein Agheli,2,3 Camilla Karlsson,2,3 Karin Ekström,2,3 Helena Brisby,3,4 Maria Lennerås,2,3 Stefan Gustafsson,3,5 Peter Sjövall,3,5,6 Anna Johansson,2,3 Eva Olsson,3,5 Jukka Lausmaa,3,6 Peter Thomsen,2,3 Sarunas Petronis3,6 1The New York Stem Cell Foundation Research Institute, New York, NY, USA; 2Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, 3BIOMATCELL VINN Excellence Center of Biomaterials and Cell Therapy, 4Department of Orthopaedics, Sahlgrenska Academy, University of Gothenburg, 5Applied Physics, Chalmers University of Technology, Göteborg, Sweden; 6Chemistry, Materials and Surfaces, SP Technical Research Institute of Sweden, Borås, Sweden Background: Patterning medical devices at the nanoscale level enables the manipulation of cell behavior and tissue regeneration, with topographic features recognized as playing a significant role in the osseointegration of implantable devices. Methods: In this study, we assessed the ability of titanium-coated hemisphere-like topographic nanostructures of different sizes (approximately 50, 100, and 200 nm) to influence the morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs). Results: We found that the proliferation and osteogenic differentiation of hMSCs was influenced by the size of the underlying structures, suggesting that size variations in topographic features at the nanoscale level, independently of chemistry, can be exploited to control hMSC behavior in a size-dependent fashion. Conclusion: Our studies demonstrate that colloidal lithography, in combination with coating technologies, can be exploited to investigate the cell response to well defined nanoscale topography and to develop next-generation surfaces that guide tissue regeneration and promote implant integration. Keywords: colloidal lithography, nanotopography, human mesenchymal stem cells, cell proliferation, osteogenic differentiation, mineralization, implantable materials
format article
author de Peppo GM
Agheli H
Karlsson C
Ekström K
Brisby H
Lennerås M
Gustafsson S
Sjövall P
Johansson A
Olsson E
Lausmaa J
Thomsen P
Petronis S
author_facet de Peppo GM
Agheli H
Karlsson C
Ekström K
Brisby H
Lennerås M
Gustafsson S
Sjövall P
Johansson A
Olsson E
Lausmaa J
Thomsen P
Petronis S
author_sort de Peppo GM
title Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
title_short Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
title_full Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
title_fullStr Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
title_full_unstemmed Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
title_sort osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/7f2270fee41d4670ba03e0671ef9a450
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