Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles

Development of Cas12a for human therapeutics and diagnostics may significantly benefit from, or even require, chemical modification of its guide RNA. Here the authors show that the noncanonical 5′ pseudoknot structure of the AsCas12a crRNA guide can be heavily modified and still retain very high edi...

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Auteurs principaux:	Eman A. Ageely, Ramadevi Chilamkurthy, Sunit Jana, Leonora Abdullahu, Daniel O’Reilly, Philip J. Jensik, Masad J. Damha, Keith T. Gagnon
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/7f23995b249f44e6bb14b6b449e1d6f8
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id	oai:doaj.org-article:7f23995b249f44e6bb14b6b449e1d6f8
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spelling	oai:doaj.org-article:7f23995b249f44e6bb14b6b449e1d6f82021-11-21T12:34:54ZGene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles10.1038/s41467-021-26989-z2041-1723https://doaj.org/article/7f23995b249f44e6bb14b6b449e1d6f82021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26989-zhttps://doaj.org/toc/2041-1723Development of Cas12a for human therapeutics and diagnostics may significantly benefit from, or even require, chemical modification of its guide RNA. Here the authors show that the noncanonical 5′ pseudoknot structure of the AsCas12a crRNA guide can be heavily modified and still retain very high editing activity in cells and trans cleavage activity in vitro.Eman A. AgeelyRamadevi ChilamkurthySunit JanaLeonora AbdullahuDaniel O’ReillyPhilip J. JensikMasad J. DamhaKeith T. GagnonNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Eman A. Ageely Ramadevi Chilamkurthy Sunit Jana Leonora Abdullahu Daniel O’Reilly Philip J. Jensik Masad J. Damha Keith T. Gagnon Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
description	Development of Cas12a for human therapeutics and diagnostics may significantly benefit from, or even require, chemical modification of its guide RNA. Here the authors show that the noncanonical 5′ pseudoknot structure of the AsCas12a crRNA guide can be heavily modified and still retain very high editing activity in cells and trans cleavage activity in vitro.
format	article
author	Eman A. Ageely Ramadevi Chilamkurthy Sunit Jana Leonora Abdullahu Daniel O’Reilly Philip J. Jensik Masad J. Damha Keith T. Gagnon
author_facet	Eman A. Ageely Ramadevi Chilamkurthy Sunit Jana Leonora Abdullahu Daniel O’Reilly Philip J. Jensik Masad J. Damha Keith T. Gagnon
author_sort	Eman A. Ageely
title	Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_short	Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_full	Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_fullStr	Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_full_unstemmed	Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_sort	gene editing with crispr-cas12a guides possessing ribose-modified pseudoknot handles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7f23995b249f44e6bb14b6b449e1d6f8
work_keys_str_mv	AT emanaageely geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT ramadevichilamkurthy geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT sunitjana geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT leonoraabdullahu geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT danieloreilly geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT philipjjensik geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT masadjdamha geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT keithtgagnon geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles
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Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles

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