Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
Development of Cas12a for human therapeutics and diagnostics may significantly benefit from, or even require, chemical modification of its guide RNA. Here the authors show that the noncanonical 5′ pseudoknot structure of the AsCas12a crRNA guide can be heavily modified and still retain very high edi...
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Nature Portfolio
2021
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oai:doaj.org-article:7f23995b249f44e6bb14b6b449e1d6f82021-11-21T12:34:54ZGene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles10.1038/s41467-021-26989-z2041-1723https://doaj.org/article/7f23995b249f44e6bb14b6b449e1d6f82021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26989-zhttps://doaj.org/toc/2041-1723Development of Cas12a for human therapeutics and diagnostics may significantly benefit from, or even require, chemical modification of its guide RNA. Here the authors show that the noncanonical 5′ pseudoknot structure of the AsCas12a crRNA guide can be heavily modified and still retain very high editing activity in cells and trans cleavage activity in vitro.Eman A. AgeelyRamadevi ChilamkurthySunit JanaLeonora AbdullahuDaniel O’ReillyPhilip J. JensikMasad J. DamhaKeith T. GagnonNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021) |
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Science Q Eman A. Ageely Ramadevi Chilamkurthy Sunit Jana Leonora Abdullahu Daniel O’Reilly Philip J. Jensik Masad J. Damha Keith T. Gagnon Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles |
description |
Development of Cas12a for human therapeutics and diagnostics may significantly benefit from, or even require, chemical modification of its guide RNA. Here the authors show that the noncanonical 5′ pseudoknot structure of the AsCas12a crRNA guide can be heavily modified and still retain very high editing activity in cells and trans cleavage activity in vitro. |
format |
article |
author |
Eman A. Ageely Ramadevi Chilamkurthy Sunit Jana Leonora Abdullahu Daniel O’Reilly Philip J. Jensik Masad J. Damha Keith T. Gagnon |
author_facet |
Eman A. Ageely Ramadevi Chilamkurthy Sunit Jana Leonora Abdullahu Daniel O’Reilly Philip J. Jensik Masad J. Damha Keith T. Gagnon |
author_sort |
Eman A. Ageely |
title |
Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles |
title_short |
Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles |
title_full |
Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles |
title_fullStr |
Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles |
title_full_unstemmed |
Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles |
title_sort |
gene editing with crispr-cas12a guides possessing ribose-modified pseudoknot handles |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/7f23995b249f44e6bb14b6b449e1d6f8 |
work_keys_str_mv |
AT emanaageely geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT ramadevichilamkurthy geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT sunitjana geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT leonoraabdullahu geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT danieloreilly geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT philipjjensik geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT masadjdamha geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles AT keithtgagnon geneeditingwithcrisprcas12aguidespossessingribosemodifiedpseudoknothandles |
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