Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles

Development of Cas12a for human therapeutics and diagnostics may significantly benefit from, or even require, chemical modification of its guide RNA. Here the authors show that the noncanonical 5′ pseudoknot structure of the AsCas12a crRNA guide can be heavily modified and still retain very high edi...

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Autores principales: Eman A. Ageely, Ramadevi Chilamkurthy, Sunit Jana, Leonora Abdullahu, Daniel O’Reilly, Philip J. Jensik, Masad J. Damha, Keith T. Gagnon
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/7f23995b249f44e6bb14b6b449e1d6f8
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spelling oai:doaj.org-article:7f23995b249f44e6bb14b6b449e1d6f82021-11-21T12:34:54ZGene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles10.1038/s41467-021-26989-z2041-1723https://doaj.org/article/7f23995b249f44e6bb14b6b449e1d6f82021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26989-zhttps://doaj.org/toc/2041-1723Development of Cas12a for human therapeutics and diagnostics may significantly benefit from, or even require, chemical modification of its guide RNA. Here the authors show that the noncanonical 5′ pseudoknot structure of the AsCas12a crRNA guide can be heavily modified and still retain very high editing activity in cells and trans cleavage activity in vitro.Eman A. AgeelyRamadevi ChilamkurthySunit JanaLeonora AbdullahuDaniel O’ReillyPhilip J. JensikMasad J. DamhaKeith T. GagnonNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Eman A. Ageely
Ramadevi Chilamkurthy
Sunit Jana
Leonora Abdullahu
Daniel O’Reilly
Philip J. Jensik
Masad J. Damha
Keith T. Gagnon
Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
description Development of Cas12a for human therapeutics and diagnostics may significantly benefit from, or even require, chemical modification of its guide RNA. Here the authors show that the noncanonical 5′ pseudoknot structure of the AsCas12a crRNA guide can be heavily modified and still retain very high editing activity in cells and trans cleavage activity in vitro.
format article
author Eman A. Ageely
Ramadevi Chilamkurthy
Sunit Jana
Leonora Abdullahu
Daniel O’Reilly
Philip J. Jensik
Masad J. Damha
Keith T. Gagnon
author_facet Eman A. Ageely
Ramadevi Chilamkurthy
Sunit Jana
Leonora Abdullahu
Daniel O’Reilly
Philip J. Jensik
Masad J. Damha
Keith T. Gagnon
author_sort Eman A. Ageely
title Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_short Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_full Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_fullStr Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_full_unstemmed Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
title_sort gene editing with crispr-cas12a guides possessing ribose-modified pseudoknot handles
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/7f23995b249f44e6bb14b6b449e1d6f8
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