Clinical Significance and Next-Generation Sequencing of Chinese Pulmonary Sarcomatoid Carcinoma

Abstract Pulmonary Sarcomatoid Carcinoma (PSC) constitutes a heterogeneous group of non-small-cell lung carcinomas (NSCLCs) with a poor prognosis. In this study, a group of 7 patients with PSC was studied. Microscope analysis of all 7 cases revealed a pleomorphic carcinoma subtype. Moreover, 5 cases...

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Autores principales:	Xin Li, Dan Wang, Qingchun Zhao, Dian Ren, Fan Ren, Gang Chen, Hongyu Liu, Jun Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f255ce762154ad9ae0a37828e1def78
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spelling	oai:doaj.org-article:7f255ce762154ad9ae0a37828e1def782021-12-02T15:05:10ZClinical Significance and Next-Generation Sequencing of Chinese Pulmonary Sarcomatoid Carcinoma10.1038/s41598-017-04296-22045-2322https://doaj.org/article/7f255ce762154ad9ae0a37828e1def782017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04296-2https://doaj.org/toc/2045-2322Abstract Pulmonary Sarcomatoid Carcinoma (PSC) constitutes a heterogeneous group of non-small-cell lung carcinomas (NSCLCs) with a poor prognosis. In this study, a group of 7 patients with PSC was studied. Microscope analysis of all 7 cases revealed a pleomorphic carcinoma subtype. Moreover, 5 cases (71.4%) were composed entirely of malignant sarcomatoid-like elements, and 2 cases (28.6%) were composed of malignant sarcomatoid-like elements and at least 10% adenocarcinoma-like elements. Immunohistochemically, the PSC components of all 7 cases were positive for vimentin and cytokeratins, including cytokeratin (CK) and cytokeratin 7 (CK7). Next-Generation Sequencing (NGS) was performed, and a total of 136 putative somatic variants and one gene fusion were identified, of which 16 variants were considered hot spot mutations, including the genes EGFR, EML4-ALK, MET, BRAF, PIK3CA, and TP53. Of these hot spot mutations, one sample expressing an EML4-ALK fusion was further confirmed by Ventana IHC, and one sample containing an EGFR exon 19 deletion was also confirmed. The NGS results imply that TP53 mutations occur often in PSCs and that EML4-ALK fusion events and EGFR exon deletions also occur in these rare tumors. Molecular targeted therapy may be a useful treatment strategy for these rare lung tumors.Xin LiDan WangQingchun ZhaoDian RenFan RenGang ChenHongyu LiuJun ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xin Li Dan Wang Qingchun Zhao Dian Ren Fan Ren Gang Chen Hongyu Liu Jun Chen Clinical Significance and Next-Generation Sequencing of Chinese Pulmonary Sarcomatoid Carcinoma
description	Abstract Pulmonary Sarcomatoid Carcinoma (PSC) constitutes a heterogeneous group of non-small-cell lung carcinomas (NSCLCs) with a poor prognosis. In this study, a group of 7 patients with PSC was studied. Microscope analysis of all 7 cases revealed a pleomorphic carcinoma subtype. Moreover, 5 cases (71.4%) were composed entirely of malignant sarcomatoid-like elements, and 2 cases (28.6%) were composed of malignant sarcomatoid-like elements and at least 10% adenocarcinoma-like elements. Immunohistochemically, the PSC components of all 7 cases were positive for vimentin and cytokeratins, including cytokeratin (CK) and cytokeratin 7 (CK7). Next-Generation Sequencing (NGS) was performed, and a total of 136 putative somatic variants and one gene fusion were identified, of which 16 variants were considered hot spot mutations, including the genes EGFR, EML4-ALK, MET, BRAF, PIK3CA, and TP53. Of these hot spot mutations, one sample expressing an EML4-ALK fusion was further confirmed by Ventana IHC, and one sample containing an EGFR exon 19 deletion was also confirmed. The NGS results imply that TP53 mutations occur often in PSCs and that EML4-ALK fusion events and EGFR exon deletions also occur in these rare tumors. Molecular targeted therapy may be a useful treatment strategy for these rare lung tumors.
format	article
author	Xin Li Dan Wang Qingchun Zhao Dian Ren Fan Ren Gang Chen Hongyu Liu Jun Chen
author_facet	Xin Li Dan Wang Qingchun Zhao Dian Ren Fan Ren Gang Chen Hongyu Liu Jun Chen
author_sort	Xin Li
title	Clinical Significance and Next-Generation Sequencing of Chinese Pulmonary Sarcomatoid Carcinoma
title_short	Clinical Significance and Next-Generation Sequencing of Chinese Pulmonary Sarcomatoid Carcinoma
title_full	Clinical Significance and Next-Generation Sequencing of Chinese Pulmonary Sarcomatoid Carcinoma
title_fullStr	Clinical Significance and Next-Generation Sequencing of Chinese Pulmonary Sarcomatoid Carcinoma
title_full_unstemmed	Clinical Significance and Next-Generation Sequencing of Chinese Pulmonary Sarcomatoid Carcinoma
title_sort	clinical significance and next-generation sequencing of chinese pulmonary sarcomatoid carcinoma
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7f255ce762154ad9ae0a37828e1def78
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Clinical Significance and Next-Generation Sequencing of Chinese Pulmonary Sarcomatoid Carcinoma

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