‘Cold shock’ increases the frequency of homology directed repair gene editing in induced pluripotent stem cells

Abstract Using CRISPR/Cas9 delivered as a RNA modality in conjunction with a lipid specifically formulated for large RNA molecules, we demonstrate that homology directed repair (HDR) rates between 20–40% can be achieved in induced pluripotent stem cells (iPSC). Furthermore, low HDR rates (between 1–...

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Autores principales: Q. Guo, G. Mintier, M. Ma-Edmonds, D. Storton, X. Wang, X. Xiao, B Kienzle, D. Zhao, John N. Feder
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/7f2a87b2a216424889cfd9d640ef048d
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