Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex

Abstract While persistence in a dormant state is crucial for the life cycle of Mycobacterium tuberculosis, no investigation regarding dormancy survival of different strains across different lineages was performed so far. We analyzed responses to oxygen starvation and recovery in terms of growth, met...

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Autores principales:	Barbara Tizzano, Tobias K. Dallenga, Christian Utpatel, Jochen Behrends, Susanne Homolka, Thomas A. Kohl, Stefan Niemann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f2f6c503bcb445e86133e23a4540b04
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spelling	oai:doaj.org-article:7f2f6c503bcb445e86133e23a4540b042021-12-02T13:57:37ZSurvival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex10.1038/s41598-021-81223-62045-2322https://doaj.org/article/7f2f6c503bcb445e86133e23a4540b042021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81223-6https://doaj.org/toc/2045-2322Abstract While persistence in a dormant state is crucial for the life cycle of Mycobacterium tuberculosis, no investigation regarding dormancy survival of different strains across different lineages was performed so far. We analyzed responses to oxygen starvation and recovery in terms of growth, metabolism, and transcription. All different strains belonging to the Euro-American lineage (L4) showed similar survival and resuscitation characteristics. Different clinical isolates from the Beijing (L2), East African-Indian (L3), and Delhi/Central Asian (L1) lineage did not survive oxygen starvation. We show that dormancy survival is lineage-dependent. Recovery from O2 starvation was only observed in strains belonging to the Euro-American (L4) lineage but not in strains belonging to different lineages (L1, L2, L3). Thus, resuscitation from dormancy after oxygen starvation is not a general feature of all M. tuberculosis strains as thought before. Our findings are of key importance to understand infection dynamics of non-Euro-American vs Euro-American strains and to develop drugs targeting the dormant state.Barbara TizzanoTobias K. DallengaChristian UtpatelJochen BehrendsSusanne HomolkaThomas A. KohlStefan NiemannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Barbara Tizzano Tobias K. Dallenga Christian Utpatel Jochen Behrends Susanne Homolka Thomas A. Kohl Stefan Niemann Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex
description	Abstract While persistence in a dormant state is crucial for the life cycle of Mycobacterium tuberculosis, no investigation regarding dormancy survival of different strains across different lineages was performed so far. We analyzed responses to oxygen starvation and recovery in terms of growth, metabolism, and transcription. All different strains belonging to the Euro-American lineage (L4) showed similar survival and resuscitation characteristics. Different clinical isolates from the Beijing (L2), East African-Indian (L3), and Delhi/Central Asian (L1) lineage did not survive oxygen starvation. We show that dormancy survival is lineage-dependent. Recovery from O2 starvation was only observed in strains belonging to the Euro-American (L4) lineage but not in strains belonging to different lineages (L1, L2, L3). Thus, resuscitation from dormancy after oxygen starvation is not a general feature of all M. tuberculosis strains as thought before. Our findings are of key importance to understand infection dynamics of non-Euro-American vs Euro-American strains and to develop drugs targeting the dormant state.
format	article
author	Barbara Tizzano Tobias K. Dallenga Christian Utpatel Jochen Behrends Susanne Homolka Thomas A. Kohl Stefan Niemann
author_facet	Barbara Tizzano Tobias K. Dallenga Christian Utpatel Jochen Behrends Susanne Homolka Thomas A. Kohl Stefan Niemann
author_sort	Barbara Tizzano
title	Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex
title_short	Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex
title_full	Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex
title_fullStr	Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex
title_full_unstemmed	Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex
title_sort	survival of hypoxia-induced dormancy is not a common feature of all strains of the mycobacterium tuberculosis complex
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7f2f6c503bcb445e86133e23a4540b04
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Survival of hypoxia-induced dormancy is not a common feature of all strains of the Mycobacterium tuberculosis complex

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