Vitamin E Supplementation Enhances Lipid Oxidative Stability via Increasing Vitamin E Retention, Rather Than Gene Expression of MAPK-Nrf2 Signaling Pathway in Muscles of Broilers

Dietary vitamin E (VE) supplementation is a method to produce VE-enriched meat and improve meat lipid oxidative stability. We aimed to study the effect of the VE supplementation duration on meat lipid oxidative stability, VE retention, and antioxidant enzymes’ activity, and explore its relationship...

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Autores principales:	Lei Xu, Jing Wang, Haijun Zhang, Shugeng Wu, Hongyuan Yue, Xiaoli Wan, Haiming Yang, Zhiyue Wang, Guanghai Qi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	VE MAPK JNK p38 Nrf2 GST Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/7f3044084b8d4344a3eb2ab2c04a2f9e
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spelling	oai:doaj.org-article:7f3044084b8d4344a3eb2ab2c04a2f9e2021-11-25T17:32:34ZVitamin E Supplementation Enhances Lipid Oxidative Stability via Increasing Vitamin E Retention, Rather Than Gene Expression of MAPK-Nrf2 Signaling Pathway in Muscles of Broilers10.3390/foods101125552304-8158https://doaj.org/article/7f3044084b8d4344a3eb2ab2c04a2f9e2021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2555https://doaj.org/toc/2304-8158Dietary vitamin E (VE) supplementation is a method to produce VE-enriched meat and improve meat lipid oxidative stability. We aimed to study the effect of the VE supplementation duration on meat lipid oxidative stability, VE retention, and antioxidant enzymes’ activity, and explore its relationship with the mitogen-activated protein kinases (MAPK)-nuclear factor-erythroid 2-related factor 2 (Nrf2) signaling pathway in broilers slaughtered after electrical stunning. A total of 240 male 18-day-old Arbor Acres Plus broilers were distributed to four treatments, with six replicates in each treatment, and ten broilers per replicate. Broilers were fed with a basal diet (no supplementation of VE) or VE diet (200 IU/kg VE, DL-α- tocopherol) for one (W1), two (W2), or three (W3) weeks before electrical stunning (130 mA, 60 Hz, for 1s) and slaughter. The VE retention was positively and linearly affected (<i>p</i> < 0.01) by the VE feeding duration at one to three weeks before slaughter, and negatively (all <i>p</i> < 0.01) related to the thiobarbituric acid reactive substance (TBARS) content in both breast and thigh muscles at d 0, d 2, and d 6 postmortem. The VE retention was negatively (<i>p</i> < 0.05) related to the gene expression of c-Jun N-terminal kinases 1 (JNK1) and 2 (JNK2), Nrf2 in breast muscles, and JNK1 and p38 MAPK in thigh muscles. In conclusion, dietary vitamin E supplementation at 200 IU/kg for three weeks before electrical stunning and slaughter improved lipid oxidative stability via increasing VE retention, rather than the regulation by gene expression of the MAPK-Nrf2 signaling pathway in skeletal muscles of broilers.Lei XuJing WangHaijun ZhangShugeng WuHongyuan YueXiaoli WanHaiming YangZhiyue WangGuanghai QiMDPI AGarticleVEMAPKJNKp38Nrf2GSTChemical technologyTP1-1185ENFoods, Vol 10, Iss 2555, p 2555 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	VE MAPK JNK p38 Nrf2 GST Chemical technology TP1-1185
spellingShingle	VE MAPK JNK p38 Nrf2 GST Chemical technology TP1-1185 Lei Xu Jing Wang Haijun Zhang Shugeng Wu Hongyuan Yue Xiaoli Wan Haiming Yang Zhiyue Wang Guanghai Qi Vitamin E Supplementation Enhances Lipid Oxidative Stability via Increasing Vitamin E Retention, Rather Than Gene Expression of MAPK-Nrf2 Signaling Pathway in Muscles of Broilers
description	Dietary vitamin E (VE) supplementation is a method to produce VE-enriched meat and improve meat lipid oxidative stability. We aimed to study the effect of the VE supplementation duration on meat lipid oxidative stability, VE retention, and antioxidant enzymes’ activity, and explore its relationship with the mitogen-activated protein kinases (MAPK)-nuclear factor-erythroid 2-related factor 2 (Nrf2) signaling pathway in broilers slaughtered after electrical stunning. A total of 240 male 18-day-old Arbor Acres Plus broilers were distributed to four treatments, with six replicates in each treatment, and ten broilers per replicate. Broilers were fed with a basal diet (no supplementation of VE) or VE diet (200 IU/kg VE, DL-α- tocopherol) for one (W1), two (W2), or three (W3) weeks before electrical stunning (130 mA, 60 Hz, for 1s) and slaughter. The VE retention was positively and linearly affected (<i>p</i> < 0.01) by the VE feeding duration at one to three weeks before slaughter, and negatively (all <i>p</i> < 0.01) related to the thiobarbituric acid reactive substance (TBARS) content in both breast and thigh muscles at d 0, d 2, and d 6 postmortem. The VE retention was negatively (<i>p</i> < 0.05) related to the gene expression of c-Jun N-terminal kinases 1 (JNK1) and 2 (JNK2), Nrf2 in breast muscles, and JNK1 and p38 MAPK in thigh muscles. In conclusion, dietary vitamin E supplementation at 200 IU/kg for three weeks before electrical stunning and slaughter improved lipid oxidative stability via increasing VE retention, rather than the regulation by gene expression of the MAPK-Nrf2 signaling pathway in skeletal muscles of broilers.
format	article
author	Lei Xu Jing Wang Haijun Zhang Shugeng Wu Hongyuan Yue Xiaoli Wan Haiming Yang Zhiyue Wang Guanghai Qi
author_facet	Lei Xu Jing Wang Haijun Zhang Shugeng Wu Hongyuan Yue Xiaoli Wan Haiming Yang Zhiyue Wang Guanghai Qi
author_sort	Lei Xu
title	Vitamin E Supplementation Enhances Lipid Oxidative Stability via Increasing Vitamin E Retention, Rather Than Gene Expression of MAPK-Nrf2 Signaling Pathway in Muscles of Broilers
title_short	Vitamin E Supplementation Enhances Lipid Oxidative Stability via Increasing Vitamin E Retention, Rather Than Gene Expression of MAPK-Nrf2 Signaling Pathway in Muscles of Broilers
title_full	Vitamin E Supplementation Enhances Lipid Oxidative Stability via Increasing Vitamin E Retention, Rather Than Gene Expression of MAPK-Nrf2 Signaling Pathway in Muscles of Broilers
title_fullStr	Vitamin E Supplementation Enhances Lipid Oxidative Stability via Increasing Vitamin E Retention, Rather Than Gene Expression of MAPK-Nrf2 Signaling Pathway in Muscles of Broilers
title_full_unstemmed	Vitamin E Supplementation Enhances Lipid Oxidative Stability via Increasing Vitamin E Retention, Rather Than Gene Expression of MAPK-Nrf2 Signaling Pathway in Muscles of Broilers
title_sort	vitamin e supplementation enhances lipid oxidative stability via increasing vitamin e retention, rather than gene expression of mapk-nrf2 signaling pathway in muscles of broilers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7f3044084b8d4344a3eb2ab2c04a2f9e
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Vitamin E Supplementation Enhances Lipid Oxidative Stability via Increasing Vitamin E Retention, Rather Than Gene Expression of MAPK-Nrf2 Signaling Pathway in Muscles of Broilers

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