Dynamics of the Type III Secretion System Activity of Enteropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>

ABSTRACT Type III secretion systems (TTSSs) are employed by pathogens to translocate host cells with effector proteins, which are crucial for virulence. The dynamics of effector translocation, behavior of the translocating bacteria, translocation temporal order, and relative amounts of each of the t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Erez Mills, Kobi Baruch, Gili Aviv, Mor Nitzan, Ilan Rosenshine
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7f3262f58908442da66a384641ec9e41
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7f3262f58908442da66a384641ec9e41
record_format	dspace
spelling	oai:doaj.org-article:7f3262f58908442da66a384641ec9e412021-11-15T15:43:09ZDynamics of the Type III Secretion System Activity of Enteropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>10.1128/mBio.00303-132150-7511https://doaj.org/article/7f3262f58908442da66a384641ec9e412013-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00303-13https://doaj.org/toc/2150-7511ABSTRACT Type III secretion systems (TTSSs) are employed by pathogens to translocate host cells with effector proteins, which are crucial for virulence. The dynamics of effector translocation, behavior of the translocating bacteria, translocation temporal order, and relative amounts of each of the translocated effectors are all poorly characterized. To address these issues, we developed a microscopy-based assay that tracks effector translocation. We used this assay alongside a previously described real-time population-based translocation assay, focusing mainly on enteropathogenic Escherichia coli (EPEC) and partly comparing it to Salmonella. We found that the two pathogens exhibit different translocation behaviors: in EPEC, a subpopulation that formed microcolonies carried out most of the translocation activity, while Salmonella executed protein translocation as planktonic bacteria. We also noted variability in host cell susceptibility, with some cells highly resistant to translocation. We next extended the study to determine the translocation dynamics of twenty EPEC effectors and found that all exhibited distinct levels of translocation efficiency. Further, we mapped the global effects of key TTSS-related components on TTSS activity. Our results provide a comprehensive description of the dynamics of the TTSS activity of EPEC and new insights into the mechanisms that control the dynamics. IMPORTANCE EPEC and the closely related enterohemorrhagic Escherichia coli (EHEC) represent a global public health problem. New strategies to combat EPEC and EHEC infections are needed, and development of such strategies requires better understanding of their virulence machinery. The TTSS is a critical virulence mechanism employed by these pathogens, and by others, including Salmonella. In this study, we aimed at elucidating new aspects of TTSS function. The results obtained provide a comprehensive description of the dynamics of TTSS activity of EPEC and new insights into the mechanisms that control these changes. This knowledge sets the stage for further analysis of the system and may accelerate the development of new ways to treat EPEC and EHEC infections. Further, the newly described microscopy-based assay can be readily adapted to study the dynamics of TTSS activity in other pathogens.Erez MillsKobi BaruchGili AvivMor NitzanIlan RosenshineAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 4 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Erez Mills Kobi Baruch Gili Aviv Mor Nitzan Ilan Rosenshine Dynamics of the Type III Secretion System Activity of Enteropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
description	ABSTRACT Type III secretion systems (TTSSs) are employed by pathogens to translocate host cells with effector proteins, which are crucial for virulence. The dynamics of effector translocation, behavior of the translocating bacteria, translocation temporal order, and relative amounts of each of the translocated effectors are all poorly characterized. To address these issues, we developed a microscopy-based assay that tracks effector translocation. We used this assay alongside a previously described real-time population-based translocation assay, focusing mainly on enteropathogenic Escherichia coli (EPEC) and partly comparing it to Salmonella. We found that the two pathogens exhibit different translocation behaviors: in EPEC, a subpopulation that formed microcolonies carried out most of the translocation activity, while Salmonella executed protein translocation as planktonic bacteria. We also noted variability in host cell susceptibility, with some cells highly resistant to translocation. We next extended the study to determine the translocation dynamics of twenty EPEC effectors and found that all exhibited distinct levels of translocation efficiency. Further, we mapped the global effects of key TTSS-related components on TTSS activity. Our results provide a comprehensive description of the dynamics of the TTSS activity of EPEC and new insights into the mechanisms that control the dynamics. IMPORTANCE EPEC and the closely related enterohemorrhagic Escherichia coli (EHEC) represent a global public health problem. New strategies to combat EPEC and EHEC infections are needed, and development of such strategies requires better understanding of their virulence machinery. The TTSS is a critical virulence mechanism employed by these pathogens, and by others, including Salmonella. In this study, we aimed at elucidating new aspects of TTSS function. The results obtained provide a comprehensive description of the dynamics of TTSS activity of EPEC and new insights into the mechanisms that control these changes. This knowledge sets the stage for further analysis of the system and may accelerate the development of new ways to treat EPEC and EHEC infections. Further, the newly described microscopy-based assay can be readily adapted to study the dynamics of TTSS activity in other pathogens.
format	article
author	Erez Mills Kobi Baruch Gili Aviv Mor Nitzan Ilan Rosenshine
author_facet	Erez Mills Kobi Baruch Gili Aviv Mor Nitzan Ilan Rosenshine
author_sort	Erez Mills
title	Dynamics of the Type III Secretion System Activity of Enteropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_short	Dynamics of the Type III Secretion System Activity of Enteropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_full	Dynamics of the Type III Secretion System Activity of Enteropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_fullStr	Dynamics of the Type III Secretion System Activity of Enteropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_full_unstemmed	Dynamics of the Type III Secretion System Activity of Enteropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>
title_sort	dynamics of the type iii secretion system activity of enteropathogenic <named-content content-type="genus-species">escherichia coli</named-content>
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/7f3262f58908442da66a384641ec9e41
work_keys_str_mv	AT erezmills dynamicsofthetypeiiisecretionsystemactivityofenteropathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontent AT kobibaruch dynamicsofthetypeiiisecretionsystemactivityofenteropathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontent AT giliaviv dynamicsofthetypeiiisecretionsystemactivityofenteropathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontent AT mornitzan dynamicsofthetypeiiisecretionsystemactivityofenteropathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontent AT ilanrosenshine dynamicsofthetypeiiisecretionsystemactivityofenteropathogenicnamedcontentcontenttypegenusspeciesescherichiacolinamedcontent
_version_	1718427594704027648

Dynamics of the Type III Secretion System Activity of Enteropathogenic <named-content content-type="genus-species">Escherichia coli</named-content>

Ejemplares similares