Feasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva

Containment of SARS-CoV-2 has become an urgent global issue. To overcome the problems of conventional quantitative polymerase chain reaction (qPCR) tests, we verified the usefulness of a mobile qPCR device that utilizes mouthwash to obtain a saliva sample with the aim of developing a rapid diagnosti...

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Autores principales:	Reiko Tokuyama-Toda, Masaaki Muraoka, Chika Terada-Ito, Shinji Ide, Toshikatsu Horiuchi, Tsuyoshi Amemiya, Airi Fukuoka, Yoshiki Hamada, Shunsuke Sejima, Kazuhito Satomura
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	COVID-19 SARS-CoV-2 mobile qPCR device mouthwash saliva Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7f339b6d00b749bbb45923d23235e97b
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spelling	oai:doaj.org-article:7f339b6d00b749bbb45923d23235e97b2021-11-25T17:20:52ZFeasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva10.3390/diagnostics111120242075-4418https://doaj.org/article/7f339b6d00b749bbb45923d23235e97b2021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/2024https://doaj.org/toc/2075-4418Containment of SARS-CoV-2 has become an urgent global issue. To overcome the problems of conventional quantitative polymerase chain reaction (qPCR) tests, we verified the usefulness of a mobile qPCR device that utilizes mouthwash to obtain a saliva sample with the aim of developing a rapid diagnostic method for SARS-CoV-2. First, we examined whether anyone could easily operate this device. Then, we examined whether RNA in the mouthwash could be detected in a short time. In addition, we investigated whether it was possible to diagnose SARS-CoV-2 infection using mouthwash obtained from COVID-19 patients undergoing hospitalization. The results revealed that all subjects were able to complete the operation properly without error. In addition, RNase P was detected in the mouthwash without pretreatment. The average detection time was 18 min, which is significantly shorter than conventional qPCR devices. Furthermore, this device detected SARS-CoV-2 in the mouthwash of a COVID-19 patient undergoing hospitalization. The above findings verified the efficacy of this diagnostic method, which had a low risk of infection, was technically simple, and provided stable results. Therefore, this method is useful for the rapid detection of SARS-CoV-2.Reiko Tokuyama-TodaMasaaki MuraokaChika Terada-ItoShinji IdeToshikatsu HoriuchiTsuyoshi AmemiyaAiri FukuokaYoshiki HamadaShunsuke SejimaKazuhito SatomuraMDPI AGarticleCOVID-19SARS-CoV-2mobile qPCR devicemouthwashsalivaMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 2024, p 2024 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 SARS-CoV-2 mobile qPCR device mouthwash saliva Medicine (General) R5-920
spellingShingle	COVID-19 SARS-CoV-2 mobile qPCR device mouthwash saliva Medicine (General) R5-920 Reiko Tokuyama-Toda Masaaki Muraoka Chika Terada-Ito Shinji Ide Toshikatsu Horiuchi Tsuyoshi Amemiya Airi Fukuoka Yoshiki Hamada Shunsuke Sejima Kazuhito Satomura Feasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva
description	Containment of SARS-CoV-2 has become an urgent global issue. To overcome the problems of conventional quantitative polymerase chain reaction (qPCR) tests, we verified the usefulness of a mobile qPCR device that utilizes mouthwash to obtain a saliva sample with the aim of developing a rapid diagnostic method for SARS-CoV-2. First, we examined whether anyone could easily operate this device. Then, we examined whether RNA in the mouthwash could be detected in a short time. In addition, we investigated whether it was possible to diagnose SARS-CoV-2 infection using mouthwash obtained from COVID-19 patients undergoing hospitalization. The results revealed that all subjects were able to complete the operation properly without error. In addition, RNase P was detected in the mouthwash without pretreatment. The average detection time was 18 min, which is significantly shorter than conventional qPCR devices. Furthermore, this device detected SARS-CoV-2 in the mouthwash of a COVID-19 patient undergoing hospitalization. The above findings verified the efficacy of this diagnostic method, which had a low risk of infection, was technically simple, and provided stable results. Therefore, this method is useful for the rapid detection of SARS-CoV-2.
format	article
author	Reiko Tokuyama-Toda Masaaki Muraoka Chika Terada-Ito Shinji Ide Toshikatsu Horiuchi Tsuyoshi Amemiya Airi Fukuoka Yoshiki Hamada Shunsuke Sejima Kazuhito Satomura
author_facet	Reiko Tokuyama-Toda Masaaki Muraoka Chika Terada-Ito Shinji Ide Toshikatsu Horiuchi Tsuyoshi Amemiya Airi Fukuoka Yoshiki Hamada Shunsuke Sejima Kazuhito Satomura
author_sort	Reiko Tokuyama-Toda
title	Feasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva
title_short	Feasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva
title_full	Feasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva
title_fullStr	Feasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva
title_full_unstemmed	Feasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva
title_sort	feasibility of rapid diagnostic technology for sars-cov-2 virus using a trace amount of saliva
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7f339b6d00b749bbb45923d23235e97b
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Feasibility of Rapid Diagnostic Technology for SARS-CoV-2 Virus Using a Trace Amount of Saliva

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