A Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia

Kanokwan Demeekul,1 Pratch Sukumolanan,2 Ratikorn Bootcha,3 Chattida Panprom,3 Soontaree Petchdee4 1Graduate School, Program of Bio-Veterinary Science, Kasetsart University, Kamphaeng Saen, Nakorn Pathom, Thailand; 2Veterinary Clinical Study Program, Faculty of Veterinary Medicine, Kasetsart Univers...

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Autores principales:	Demeekul K, Sukumolanan P, Bootcha R, Panprom C, Petchdee S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	cardioprotection cardiopulmonary bypass germinated brown rice ischemic injury primary porcine cardiomyocytes Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/7f37ba81100f4cfe9c666a832ec18a42
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spelling	oai:doaj.org-article:7f37ba81100f4cfe9c666a832ec18a422021-12-02T18:31:14ZA Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia1178-7031https://doaj.org/article/7f37ba81100f4cfe9c666a832ec18a422021-07-01T00:00:00Zhttps://www.dovepress.com/a-cardiac-protection-of-germinated-brown-rice-during-cardiopulmonary-b-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Kanokwan Demeekul,1 Pratch Sukumolanan,2 Ratikorn Bootcha,3 Chattida Panprom,3 Soontaree Petchdee4 1Graduate School, Program of Bio-Veterinary Science, Kasetsart University, Kamphaeng Saen, Nakorn Pathom, Thailand; 2Veterinary Clinical Study Program, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakorn Pathom, Thailand; 3Kasetsart University Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Kamphaeng Saen, Nakorn Pathom, Thailand; 4Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, KamphaengSaen Campus, Kamphaeng Saen, ThailandCorrespondence: Soontaree PetchdeeDepartment of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, KamphaengSaen Campus, Kamphaeng Saen, Nakorn Pathom, 73140, ThailandTel +66 34 351901-3Fax +66 34 351405Email fvetstr@ku.ac.thPurpose: The potential cardio-protective property of germinated brown rice (GBR) has been revealed by ameliorating risk factors related to cardiovascular diseases. This study hypothesized that the combination of GBR and cardioplegic solution could protect the cardiomyocytes exposed to simulated ischemic reperfusion injury in vitro study and preserve cardiac function during cardiopulmonary bypass surgery in animal models.Methods: Primary porcine cardiomyocytes were isolated and experimented cell viability against simulated ischemic reperfusion injury. In a cardiac surgical animal model, six pigs were randomly assigned to receive the two types of cardioplegic solution: i) St. Thomas cardioplegic solution (20 cc/kg); and ii) St. Thomas cardioplegic solution plus GBR (1 mg/kg). During open-heart surgery, the aorta was cross-clamped for 20 minutes, followed by reperfusion for 1 hour. Cardiopulmonary bypass parameters were recorded until the end of the procedure. Furthermore, hemodynamic parameters and arterial blood gas characteristics of animals among groups were monitored at different time points, including baseline before cardiopulmonary bypass (T1), during cardiopulmonary bypass (T2), during aortic clamp on (T3), and aortic clamp off (T4).Results: Primarily, GBR cotreatment with cardioplegic solution essentially resulted in the improvement of cell viability in primary porcine cardiomyocytes against simulated ischemic reperfusion induction. The findings from cardiac surgery demonstrated that mean arterial pressure and heart rate are constantly stable in cardioplegic solution combined with the GBR group, while the trend of potassium and lactase concentration was decreased in the animals receiving GBR group. Consistently, all parameters from arterial blood gas showed better outcomes in animals receiving GBR; however, there were no statistically significant differences between groups, except hepatic enzymes.Conclusion: Therefore, GBR might exert cardio-protective effects against ischemic reperfusion injury in the porcine cardiac surgery model due to anti-inflammatory response. These protective actions of GBR may explain the benefits gained from applying GBR products as a possible therapeutic supplement on cardiac diseases.Keywords: cardioprotection, cardiopulmonary bypass, germinated brown rice, ischemic injury, primary porcine cardiomyocytesDemeekul KSukumolanan PBootcha RPanprom CPetchdee SDove Medical Pressarticlecardioprotectioncardiopulmonary bypassgerminated brown riceischemic injuryprimary porcine cardiomyocytesPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 3307-3319 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cardioprotection cardiopulmonary bypass germinated brown rice ischemic injury primary porcine cardiomyocytes Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	cardioprotection cardiopulmonary bypass germinated brown rice ischemic injury primary porcine cardiomyocytes Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Demeekul K Sukumolanan P Bootcha R Panprom C Petchdee S A Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia
description	Kanokwan Demeekul,1 Pratch Sukumolanan,2 Ratikorn Bootcha,3 Chattida Panprom,3 Soontaree Petchdee4 1Graduate School, Program of Bio-Veterinary Science, Kasetsart University, Kamphaeng Saen, Nakorn Pathom, Thailand; 2Veterinary Clinical Study Program, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakorn Pathom, Thailand; 3Kasetsart University Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Kamphaeng Saen, Nakorn Pathom, Thailand; 4Department of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, KamphaengSaen Campus, Kamphaeng Saen, ThailandCorrespondence: Soontaree PetchdeeDepartment of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, KamphaengSaen Campus, Kamphaeng Saen, Nakorn Pathom, 73140, ThailandTel +66 34 351901-3Fax +66 34 351405Email fvetstr@ku.ac.thPurpose: The potential cardio-protective property of germinated brown rice (GBR) has been revealed by ameliorating risk factors related to cardiovascular diseases. This study hypothesized that the combination of GBR and cardioplegic solution could protect the cardiomyocytes exposed to simulated ischemic reperfusion injury in vitro study and preserve cardiac function during cardiopulmonary bypass surgery in animal models.Methods: Primary porcine cardiomyocytes were isolated and experimented cell viability against simulated ischemic reperfusion injury. In a cardiac surgical animal model, six pigs were randomly assigned to receive the two types of cardioplegic solution: i) St. Thomas cardioplegic solution (20 cc/kg); and ii) St. Thomas cardioplegic solution plus GBR (1 mg/kg). During open-heart surgery, the aorta was cross-clamped for 20 minutes, followed by reperfusion for 1 hour. Cardiopulmonary bypass parameters were recorded until the end of the procedure. Furthermore, hemodynamic parameters and arterial blood gas characteristics of animals among groups were monitored at different time points, including baseline before cardiopulmonary bypass (T1), during cardiopulmonary bypass (T2), during aortic clamp on (T3), and aortic clamp off (T4).Results: Primarily, GBR cotreatment with cardioplegic solution essentially resulted in the improvement of cell viability in primary porcine cardiomyocytes against simulated ischemic reperfusion induction. The findings from cardiac surgery demonstrated that mean arterial pressure and heart rate are constantly stable in cardioplegic solution combined with the GBR group, while the trend of potassium and lactase concentration was decreased in the animals receiving GBR group. Consistently, all parameters from arterial blood gas showed better outcomes in animals receiving GBR; however, there were no statistically significant differences between groups, except hepatic enzymes.Conclusion: Therefore, GBR might exert cardio-protective effects against ischemic reperfusion injury in the porcine cardiac surgery model due to anti-inflammatory response. These protective actions of GBR may explain the benefits gained from applying GBR products as a possible therapeutic supplement on cardiac diseases.Keywords: cardioprotection, cardiopulmonary bypass, germinated brown rice, ischemic injury, primary porcine cardiomyocytes
format	article
author	Demeekul K Sukumolanan P Bootcha R Panprom C Petchdee S
author_facet	Demeekul K Sukumolanan P Bootcha R Panprom C Petchdee S
author_sort	Demeekul K
title	A Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia
title_short	A Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia
title_full	A Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia
title_fullStr	A Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia
title_full_unstemmed	A Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia
title_sort	cardiac protection of germinated brown rice during cardiopulmonary bypass surgery and simulated myocardial ischemia
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/7f37ba81100f4cfe9c666a832ec18a42
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A Cardiac Protection of Germinated Brown Rice During Cardiopulmonary Bypass Surgery and Simulated Myocardial Ischemia

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