Identification of hypoxia-induced genes in human SGBS adipocytes by microarray analysis.

Hypoxia in adipose tissue is suggested to be involved in the development of a chronic mild inflammation, which in obesity can further lead to insulin resistance. The effect of hypoxia on gene expression in adipocytes appears to play a central role in this inflammatory response observed in obesity. H...

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Autores principales:	Kathrin Geiger, Andreas Leiherer, Axel Muendlein, Nicole Stark, Simone Geller-Rhomberg, Christoph H Saely, Martin Wabitsch, Peter Fraunberger, Heinz Drexel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f3feae6afcc4157a1a8834b805b2be9
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spelling	oai:doaj.org-article:7f3feae6afcc4157a1a8834b805b2be92021-11-18T07:36:06ZIdentification of hypoxia-induced genes in human SGBS adipocytes by microarray analysis.1932-620310.1371/journal.pone.0026465https://doaj.org/article/7f3feae6afcc4157a1a8834b805b2be92011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039494/?tool=EBIhttps://doaj.org/toc/1932-6203Hypoxia in adipose tissue is suggested to be involved in the development of a chronic mild inflammation, which in obesity can further lead to insulin resistance. The effect of hypoxia on gene expression in adipocytes appears to play a central role in this inflammatory response observed in obesity. However, the global impact of hypoxia on transcriptional changes in human adipocytes is unclear. Therefore, we compared gene expression profiles of human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes under normoxic or hypoxic conditions to detect hypoxia-responsive genes in adipocytes by using whole human genome microarrays. Microarray analysis showed more than 500 significantly differentially regulated mRNAs after incubation of the cells under low oxygen levels. To gain further insight into the biological processes, hypoxia-regulated genes after 16 hours of hypoxia were classified according to their function. We identified an enrichment of genes involved in important biological processes such as glycolysis, response to hypoxia, regulation of cellular component movement, response to nutrient levels, regulation of cell migration, and transcription regulator activity. Real-time PCR confirmed eight genes to be consistently upregulated in response to 3, 6 and 16 hours of hypoxia. For adipocytes the hypoxia-induced regulation of these genes is shown here for the first time. Moreover in six of these eight genes we identified HIF response elements in the proximal promoters, specific for the HIF transcription factor family members HIF1A and HIF2A. In the present study, we demonstrated that hypoxia has an extensive effect on gene expression of SGBS adipocytes. In addition, the identified hypoxia-regulated genes are likely involved in the regulation of obesity, the incidence of type 2 diabetes, and the metabolic syndrome.Kathrin GeigerAndreas LeihererAxel MuendleinNicole StarkSimone Geller-RhombergChristoph H SaelyMartin WabitschPeter FraunbergerHeinz DrexelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26465 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kathrin Geiger Andreas Leiherer Axel Muendlein Nicole Stark Simone Geller-Rhomberg Christoph H Saely Martin Wabitsch Peter Fraunberger Heinz Drexel Identification of hypoxia-induced genes in human SGBS adipocytes by microarray analysis.
description	Hypoxia in adipose tissue is suggested to be involved in the development of a chronic mild inflammation, which in obesity can further lead to insulin resistance. The effect of hypoxia on gene expression in adipocytes appears to play a central role in this inflammatory response observed in obesity. However, the global impact of hypoxia on transcriptional changes in human adipocytes is unclear. Therefore, we compared gene expression profiles of human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes under normoxic or hypoxic conditions to detect hypoxia-responsive genes in adipocytes by using whole human genome microarrays. Microarray analysis showed more than 500 significantly differentially regulated mRNAs after incubation of the cells under low oxygen levels. To gain further insight into the biological processes, hypoxia-regulated genes after 16 hours of hypoxia were classified according to their function. We identified an enrichment of genes involved in important biological processes such as glycolysis, response to hypoxia, regulation of cellular component movement, response to nutrient levels, regulation of cell migration, and transcription regulator activity. Real-time PCR confirmed eight genes to be consistently upregulated in response to 3, 6 and 16 hours of hypoxia. For adipocytes the hypoxia-induced regulation of these genes is shown here for the first time. Moreover in six of these eight genes we identified HIF response elements in the proximal promoters, specific for the HIF transcription factor family members HIF1A and HIF2A. In the present study, we demonstrated that hypoxia has an extensive effect on gene expression of SGBS adipocytes. In addition, the identified hypoxia-regulated genes are likely involved in the regulation of obesity, the incidence of type 2 diabetes, and the metabolic syndrome.
format	article
author	Kathrin Geiger Andreas Leiherer Axel Muendlein Nicole Stark Simone Geller-Rhomberg Christoph H Saely Martin Wabitsch Peter Fraunberger Heinz Drexel
author_facet	Kathrin Geiger Andreas Leiherer Axel Muendlein Nicole Stark Simone Geller-Rhomberg Christoph H Saely Martin Wabitsch Peter Fraunberger Heinz Drexel
author_sort	Kathrin Geiger
title	Identification of hypoxia-induced genes in human SGBS adipocytes by microarray analysis.
title_short	Identification of hypoxia-induced genes in human SGBS adipocytes by microarray analysis.
title_full	Identification of hypoxia-induced genes in human SGBS adipocytes by microarray analysis.
title_fullStr	Identification of hypoxia-induced genes in human SGBS adipocytes by microarray analysis.
title_full_unstemmed	Identification of hypoxia-induced genes in human SGBS adipocytes by microarray analysis.
title_sort	identification of hypoxia-induced genes in human sgbs adipocytes by microarray analysis.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/7f3feae6afcc4157a1a8834b805b2be9
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Identification of hypoxia-induced genes in human SGBS adipocytes by microarray analysis.

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