Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends

The interaction between proteins and drugs or other bioactive compounds has been widely explored over the past years. Several methods for analysis of this phenomenon have been developed and improved. Nowadays, increasing attention is paid to innovative methods, such as high performance affinity liqu...

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Autores principales:	Tony Cardoso, Ana Sofia Almeida, Fernando Remião, Carla Fernandes
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	affinity capillary electrophoresis binding affinity docking enantiomeric separation enantioselectivity high performance affinity liquid chromatography Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/7f40a4462b5d464cb315583655ca4a9b
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spelling	oai:doaj.org-article:7f40a4462b5d464cb315583655ca4a9b2021-11-25T17:13:32ZEnantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends10.3390/chemosensors91103042227-9040https://doaj.org/article/7f40a4462b5d464cb315583655ca4a9b2021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9040/9/11/304https://doaj.org/toc/2227-9040The interaction between proteins and drugs or other bioactive compounds has been widely explored over the past years. Several methods for analysis of this phenomenon have been developed and improved. Nowadays, increasing attention is paid to innovative methods, such as high performance affinity liquid chromatography (HPALC) and affinity capillary electrophoresis (ACE), taking into account various advantages. Moreover, the development of separation methods for the analysis and resolution of chiral drugs has been an area of ongoing interest in analytical and medicinal chemistry research. In addition to bioaffinity binding studies, both HPALC and ACE al-low one to perform other type of analyses, namely, displacement studies and enantioseparation of racemic or enantiomeric mixtures. Actually, proteins used as chiral selectors in chromatographic and electrophoretic methods have unique enantioselective properties demonstrating suitability for the enantioseparation of a large variety of chiral drugs or other bioactive compounds. This review is mainly focused in chromatographic and electrophoretic methods using human serum albumin (HSA), the most abundant plasma protein, as chiral selector for binding affinity analysis and enantioresolution of drugs. For both analytical purposes, updated examples are presented to highlight recent applications and current trends.Tony CardosoAna Sofia AlmeidaFernando RemiãoCarla FernandesMDPI AGarticleaffinity capillary electrophoresisbinding affinitydockingenantiomeric separationenantioselectivityhigh performance affinity liquid chromatographyBiochemistryQD415-436ENChemosensors, Vol 9, Iss 304, p 304 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	affinity capillary electrophoresis binding affinity docking enantiomeric separation enantioselectivity high performance affinity liquid chromatography Biochemistry QD415-436
spellingShingle	affinity capillary electrophoresis binding affinity docking enantiomeric separation enantioselectivity high performance affinity liquid chromatography Biochemistry QD415-436 Tony Cardoso Ana Sofia Almeida Fernando Remião Carla Fernandes Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends
description	The interaction between proteins and drugs or other bioactive compounds has been widely explored over the past years. Several methods for analysis of this phenomenon have been developed and improved. Nowadays, increasing attention is paid to innovative methods, such as high performance affinity liquid chromatography (HPALC) and affinity capillary electrophoresis (ACE), taking into account various advantages. Moreover, the development of separation methods for the analysis and resolution of chiral drugs has been an area of ongoing interest in analytical and medicinal chemistry research. In addition to bioaffinity binding studies, both HPALC and ACE al-low one to perform other type of analyses, namely, displacement studies and enantioseparation of racemic or enantiomeric mixtures. Actually, proteins used as chiral selectors in chromatographic and electrophoretic methods have unique enantioselective properties demonstrating suitability for the enantioseparation of a large variety of chiral drugs or other bioactive compounds. This review is mainly focused in chromatographic and electrophoretic methods using human serum albumin (HSA), the most abundant plasma protein, as chiral selector for binding affinity analysis and enantioresolution of drugs. For both analytical purposes, updated examples are presented to highlight recent applications and current trends.
format	article
author	Tony Cardoso Ana Sofia Almeida Fernando Remião Carla Fernandes
author_facet	Tony Cardoso Ana Sofia Almeida Fernando Remião Carla Fernandes
author_sort	Tony Cardoso
title	Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends
title_short	Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends
title_full	Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends
title_fullStr	Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends
title_full_unstemmed	Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends
title_sort	enantioresolution and binding affinity studies on human serum albumin: recent applications and trends
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7f40a4462b5d464cb315583655ca4a9b
work_keys_str_mv	AT tonycardoso enantioresolutionandbindingaffinitystudiesonhumanserumalbuminrecentapplicationsandtrends AT anasofiaalmeida enantioresolutionandbindingaffinitystudiesonhumanserumalbuminrecentapplicationsandtrends AT fernandoremiao enantioresolutionandbindingaffinitystudiesonhumanserumalbuminrecentapplicationsandtrends AT carlafernandes enantioresolutionandbindingaffinitystudiesonhumanserumalbuminrecentapplicationsandtrends
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Enantioresolution and Binding Affinity Studies on Human Serum Albumin: Recent Applications and Trends

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