Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria

This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of <i>Rhodococcus erythropolis</i> and one <i>Pseudomonas</i> sp.), isolated from the NW Portuguese coast, we...

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Autores principales:	Rafaela Perdigão, C. Marisa R. Almeida, Catarina Magalhães, Sandra Ramos, Ana L. Carolas, Bruno S. Ferreira, Maria F. Carvalho, Ana P. Mucha
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	autochthonous bioremediation oil spills hydrocarbons bioaugmentation bioremediation agent lyophilized bacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7f5d1210f99d4e339e295e50e458285e
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spelling	oai:doaj.org-article:7f5d1210f99d4e339e295e50e458285e2021-11-25T18:24:51ZBioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria10.3390/microorganisms91122852076-2607https://doaj.org/article/7f5d1210f99d4e339e295e50e458285e2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2285https://doaj.org/toc/2076-2607This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of <i>Rhodococcus erythropolis</i> and one <i>Pseudomonas</i> sp.), isolated from the NW Portuguese coast, were selected for lyophilization after biomass growth optimization (tested with alternative carbon sources). Results indicated that the bacterial strains remained viable after the lyophilization process, without losing their biodegradation potential. The biomass/petroleum ratio was optimized, and the bioremediation efficiency of the lyophilized bacterial consortium was tested in microcosms with natural seawater and petroleum. An acceleration of the natural oil degradation process was observed, with an increased abundance of oil-degraders after 24 h, an emulsion of the oil/water layer after 7 days, and an increased removal of total petroleum hydrocarbons (47%) after 15 days. This study provides an insight into the formulation and optimization of lyophilized bacterial agents for application in autochthonous oil bioremediation.Rafaela PerdigãoC. Marisa R. AlmeidaCatarina MagalhãesSandra RamosAna L. CarolasBruno S. FerreiraMaria F. CarvalhoAna P. MuchaMDPI AGarticleautochthonous bioremediationoil spillshydrocarbonsbioaugmentationbioremediation agentlyophilized bacteriaBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2285, p 2285 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	autochthonous bioremediation oil spills hydrocarbons bioaugmentation bioremediation agent lyophilized bacteria Biology (General) QH301-705.5
spellingShingle	autochthonous bioremediation oil spills hydrocarbons bioaugmentation bioremediation agent lyophilized bacteria Biology (General) QH301-705.5 Rafaela Perdigão C. Marisa R. Almeida Catarina Magalhães Sandra Ramos Ana L. Carolas Bruno S. Ferreira Maria F. Carvalho Ana P. Mucha Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria
description	This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of <i>Rhodococcus erythropolis</i> and one <i>Pseudomonas</i> sp.), isolated from the NW Portuguese coast, were selected for lyophilization after biomass growth optimization (tested with alternative carbon sources). Results indicated that the bacterial strains remained viable after the lyophilization process, without losing their biodegradation potential. The biomass/petroleum ratio was optimized, and the bioremediation efficiency of the lyophilized bacterial consortium was tested in microcosms with natural seawater and petroleum. An acceleration of the natural oil degradation process was observed, with an increased abundance of oil-degraders after 24 h, an emulsion of the oil/water layer after 7 days, and an increased removal of total petroleum hydrocarbons (47%) after 15 days. This study provides an insight into the formulation and optimization of lyophilized bacterial agents for application in autochthonous oil bioremediation.
format	article
author	Rafaela Perdigão C. Marisa R. Almeida Catarina Magalhães Sandra Ramos Ana L. Carolas Bruno S. Ferreira Maria F. Carvalho Ana P. Mucha
author_facet	Rafaela Perdigão C. Marisa R. Almeida Catarina Magalhães Sandra Ramos Ana L. Carolas Bruno S. Ferreira Maria F. Carvalho Ana P. Mucha
author_sort	Rafaela Perdigão
title	Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria
title_short	Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria
title_full	Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria
title_fullStr	Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria
title_full_unstemmed	Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria
title_sort	bioremediation of petroleum hydrocarbons in seawater: prospects of using lyophilized native hydrocarbon-degrading bacteria
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7f5d1210f99d4e339e295e50e458285e
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Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria

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