Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel

Abstract Multispecies microbial adherent communities are widespread in nature and organisms, although the principles of their assembly and development remain unclear. Here, we test the possibility of establishing a simplified but relevant model of multispecies biofilm in a non-invasive laboratory se...

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Autores principales:	A. Monmeyran, W. Benyoussef, P. Thomen, N. Dahmane, A. Baliarda, M. Jules, S. Aymerich, N. Henry
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/7f6100d82d10470fbdbb56af6583c0a7
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spelling	oai:doaj.org-article:7f6100d82d10470fbdbb56af6583c0a72021-12-02T14:53:50ZFour species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel10.1038/s41522-021-00233-42055-5008https://doaj.org/article/7f6100d82d10470fbdbb56af6583c0a72021-08-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00233-4https://doaj.org/toc/2055-5008Abstract Multispecies microbial adherent communities are widespread in nature and organisms, although the principles of their assembly and development remain unclear. Here, we test the possibility of establishing a simplified but relevant model of multispecies biofilm in a non-invasive laboratory setup for the real-time monitoring of community development. We demonstrate that the four chosen species (Bacillus thuringiensis, Pseudomonas fluorescens, Kocuria varians, and Rhodocyclus sp.) form a dynamic community that deterministically reaches its equilibrium after ~30 h of growth. We reveal the emergence of complexity in this simplified community as reported by an increase in spatial heterogeneity and non-monotonic developmental kinetics. Importantly, we find interspecies interactions consisting of competition for resources—particularly oxygen—and both direct and indirect physical interactions. The simplified experimental model opens new avenues to the study of adherent bacterial communities and their behavior in the context of rapid global change.A. MonmeyranW. BenyoussefP. ThomenN. DahmaneA. BaliardaM. JulesS. AymerichN. HenryNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 A. Monmeyran W. Benyoussef P. Thomen N. Dahmane A. Baliarda M. Jules S. Aymerich N. Henry Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel
description	Abstract Multispecies microbial adherent communities are widespread in nature and organisms, although the principles of their assembly and development remain unclear. Here, we test the possibility of establishing a simplified but relevant model of multispecies biofilm in a non-invasive laboratory setup for the real-time monitoring of community development. We demonstrate that the four chosen species (Bacillus thuringiensis, Pseudomonas fluorescens, Kocuria varians, and Rhodocyclus sp.) form a dynamic community that deterministically reaches its equilibrium after ~30 h of growth. We reveal the emergence of complexity in this simplified community as reported by an increase in spatial heterogeneity and non-monotonic developmental kinetics. Importantly, we find interspecies interactions consisting of competition for resources—particularly oxygen—and both direct and indirect physical interactions. The simplified experimental model opens new avenues to the study of adherent bacterial communities and their behavior in the context of rapid global change.
format	article
author	A. Monmeyran W. Benyoussef P. Thomen N. Dahmane A. Baliarda M. Jules S. Aymerich N. Henry
author_facet	A. Monmeyran W. Benyoussef P. Thomen N. Dahmane A. Baliarda M. Jules S. Aymerich N. Henry
author_sort	A. Monmeyran
title	Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel
title_short	Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel
title_full	Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel
title_fullStr	Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel
title_full_unstemmed	Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel
title_sort	four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7f6100d82d10470fbdbb56af6583c0a7
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Four species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel

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