Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas

Abstract Lymphoma is the most common type of canine hematological malignancy where the multicentric (cMCL) form accounts for 75% of all cases. The standard treatment is the CHOP chemotherapy protocols that include cyclophosphamide, doxorubicin, vincristine and prednisone, where the majority of dogs...

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Autores principales:	Taismara K. Garnica, Jéssika C. C. Lesbon, Ana C. F. C. M. Ávila, Arina L. Rochetti, Oscar R. S. Matiz, Roana C. S. Ribeiro, Aline Zoppa, Adriana T. Nishiya, Mirela T. Costa, Andrigo B. de Nardi, David J. Argyle, Ricardo F. Strefezzi, Juliano C. Silveira, Heidge Fukumasu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f611e4eb68c40a7851795778365a7f3
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spelling	oai:doaj.org-article:7f611e4eb68c40a7851795778365a7f32021-12-02T12:33:52ZLiquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas10.1038/s41598-020-77366-72045-2322https://doaj.org/article/7f611e4eb68c40a7851795778365a7f32020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77366-7https://doaj.org/toc/2045-2322Abstract Lymphoma is the most common type of canine hematological malignancy where the multicentric (cMCL) form accounts for 75% of all cases. The standard treatment is the CHOP chemotherapy protocols that include cyclophosphamide, doxorubicin, vincristine and prednisone, where the majority of dogs achieve complete/partial response; however, it is very important to predict non-responsive cases to improve treatment and to develop new targeted therapies. Here we evaluate a liquid biopsy approach based on serum Small Extracellular Vesicles enriched for exosomes (SEVs) to predict cMCL chemotherapy response. Nineteen dogs at the end of the 19-week chemotherapy protocol (8 Complete Response and 11 Progressive Disease) were evaluated for serum SEVs size, concentration and screened for 95 oncomirs. PD patients had higher SEVs concentration at the diagnosis than CR patients (P = 0.034). The ROC curve was significant for SEVs concentration to predict the response to CHOP (AUC = 0.8011, P = 0.0287). A potential molecular signature based on oncomirs from SEVs (caf-miR-205, caf-miR-222, caf-mir-20a and caf-miR-93) is proposed. To the best of our knowledge, this is the first study demonstrating the potential of a liquid biopsy based on SEVs and their miRNAs content to predict the outcome of chemotherapy for canine multicentric lymphomas.Taismara K. GarnicaJéssika C. C. LesbonAna C. F. C. M. ÁvilaArina L. RochettiOscar R. S. MatizRoana C. S. RibeiroAline ZoppaAdriana T. NishiyaMirela T. CostaAndrigo B. de NardiDavid J. ArgyleRicardo F. StrefezziJuliano C. SilveiraHeidge FukumasuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taismara K. Garnica Jéssika C. C. Lesbon Ana C. F. C. M. Ávila Arina L. Rochetti Oscar R. S. Matiz Roana C. S. Ribeiro Aline Zoppa Adriana T. Nishiya Mirela T. Costa Andrigo B. de Nardi David J. Argyle Ricardo F. Strefezzi Juliano C. Silveira Heidge Fukumasu Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas
description	Abstract Lymphoma is the most common type of canine hematological malignancy where the multicentric (cMCL) form accounts for 75% of all cases. The standard treatment is the CHOP chemotherapy protocols that include cyclophosphamide, doxorubicin, vincristine and prednisone, where the majority of dogs achieve complete/partial response; however, it is very important to predict non-responsive cases to improve treatment and to develop new targeted therapies. Here we evaluate a liquid biopsy approach based on serum Small Extracellular Vesicles enriched for exosomes (SEVs) to predict cMCL chemotherapy response. Nineteen dogs at the end of the 19-week chemotherapy protocol (8 Complete Response and 11 Progressive Disease) were evaluated for serum SEVs size, concentration and screened for 95 oncomirs. PD patients had higher SEVs concentration at the diagnosis than CR patients (P = 0.034). The ROC curve was significant for SEVs concentration to predict the response to CHOP (AUC = 0.8011, P = 0.0287). A potential molecular signature based on oncomirs from SEVs (caf-miR-205, caf-miR-222, caf-mir-20a and caf-miR-93) is proposed. To the best of our knowledge, this is the first study demonstrating the potential of a liquid biopsy based on SEVs and their miRNAs content to predict the outcome of chemotherapy for canine multicentric lymphomas.
format	article
author	Taismara K. Garnica Jéssika C. C. Lesbon Ana C. F. C. M. Ávila Arina L. Rochetti Oscar R. S. Matiz Roana C. S. Ribeiro Aline Zoppa Adriana T. Nishiya Mirela T. Costa Andrigo B. de Nardi David J. Argyle Ricardo F. Strefezzi Juliano C. Silveira Heidge Fukumasu
author_facet	Taismara K. Garnica Jéssika C. C. Lesbon Ana C. F. C. M. Ávila Arina L. Rochetti Oscar R. S. Matiz Roana C. S. Ribeiro Aline Zoppa Adriana T. Nishiya Mirela T. Costa Andrigo B. de Nardi David J. Argyle Ricardo F. Strefezzi Juliano C. Silveira Heidge Fukumasu
author_sort	Taismara K. Garnica
title	Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas
title_short	Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas
title_full	Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas
title_fullStr	Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas
title_full_unstemmed	Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas
title_sort	liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7f611e4eb68c40a7851795778365a7f3
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Liquid biopsy based on small extracellular vesicles predicts chemotherapy response of canine multicentric lymphomas

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