New thermodynamic activity-based approach allows predicting the feasibility of glycolysis

Abstract Thermodynamic feasibility analyses help evaluating the feasibility of metabolic pathways. This is an important information used to develop new biotechnological processes and to understand metabolic processes in cells. However, literature standard data are uncertain for most biochemical reac...

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Autores principales:	Thorsten Greinert, Kristina Vogel, Thomas Maskow, Christoph Held
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f6345fded7a45da9eaa1697e1f8ff71
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spelling	oai:doaj.org-article:7f6345fded7a45da9eaa1697e1f8ff712021-12-02T17:05:45ZNew thermodynamic activity-based approach allows predicting the feasibility of glycolysis10.1038/s41598-021-85594-82045-2322https://doaj.org/article/7f6345fded7a45da9eaa1697e1f8ff712021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85594-8https://doaj.org/toc/2045-2322Abstract Thermodynamic feasibility analyses help evaluating the feasibility of metabolic pathways. This is an important information used to develop new biotechnological processes and to understand metabolic processes in cells. However, literature standard data are uncertain for most biochemical reactions yielding wrong statements concerning their feasibility. In this article we present activity-based equilibrium constants for all the ten glycolytic reactions, accompanied by the standard reaction data (standard Gibbs energy of reaction and standard enthalpy of reaction). We further developed a thermodynamic activity-based approach that allows to correctly determine the feasibility of glycolysis under different chosen conditions. The results show for the first time that the feasibility of glycolysis can be explained by thermodynamics only if (1) correct standard data are used and if (2) the conditions in the cell at non-equilibrium states are accounted for in the analyses. The results here will help to determine the feasibility of other metabolisms and to understand metabolic processes in cells in the future.Thorsten GreinertKristina VogelThomas MaskowChristoph HeldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thorsten Greinert Kristina Vogel Thomas Maskow Christoph Held New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
description	Abstract Thermodynamic feasibility analyses help evaluating the feasibility of metabolic pathways. This is an important information used to develop new biotechnological processes and to understand metabolic processes in cells. However, literature standard data are uncertain for most biochemical reactions yielding wrong statements concerning their feasibility. In this article we present activity-based equilibrium constants for all the ten glycolytic reactions, accompanied by the standard reaction data (standard Gibbs energy of reaction and standard enthalpy of reaction). We further developed a thermodynamic activity-based approach that allows to correctly determine the feasibility of glycolysis under different chosen conditions. The results show for the first time that the feasibility of glycolysis can be explained by thermodynamics only if (1) correct standard data are used and if (2) the conditions in the cell at non-equilibrium states are accounted for in the analyses. The results here will help to determine the feasibility of other metabolisms and to understand metabolic processes in cells in the future.
format	article
author	Thorsten Greinert Kristina Vogel Thomas Maskow Christoph Held
author_facet	Thorsten Greinert Kristina Vogel Thomas Maskow Christoph Held
author_sort	Thorsten Greinert
title	New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
title_short	New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
title_full	New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
title_fullStr	New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
title_full_unstemmed	New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
title_sort	new thermodynamic activity-based approach allows predicting the feasibility of glycolysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7f6345fded7a45da9eaa1697e1f8ff71
work_keys_str_mv	AT thorstengreinert newthermodynamicactivitybasedapproachallowspredictingthefeasibilityofglycolysis AT kristinavogel newthermodynamicactivitybasedapproachallowspredictingthefeasibilityofglycolysis AT thomasmaskow newthermodynamicactivitybasedapproachallowspredictingthefeasibilityofglycolysis AT christophheld newthermodynamicactivitybasedapproachallowspredictingthefeasibilityofglycolysis
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New thermodynamic activity-based approach allows predicting the feasibility of glycolysis

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