IN VITRO METHOD FOR EXPRESSION OF TOXIN PRODUCTION IN NON-TOXIGENIC CORYNEBACTERIUM SPP. WITH “SILENT” TOX-GENE

The «in vitro method» for expression of toxin-production by phenotypically non-toxigenic strains of C. diphtheriae containing the “silent” toxin gene has been developed. The method can be characterised as rapid, economical, not demanding use of experimental animals, available to practical and scient...

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Autor principal:	S. A. Gabrielyan
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2015
Materias:	diphtheritic toxin «silent» toxin gene restoration of toxin-producing Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/7f64e8c2aff54a1ca082d46e16880dd0
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spelling	oai:doaj.org-article:7f64e8c2aff54a1ca082d46e16880dd02021-11-22T07:09:47ZIN VITRO METHOD FOR EXPRESSION OF TOXIN PRODUCTION IN NON-TOXIGENIC CORYNEBACTERIUM SPP. WITH “SILENT” TOX-GENE2220-76192313-739810.15789/2220-7619-2014-4-375-380https://doaj.org/article/7f64e8c2aff54a1ca082d46e16880dd02015-02-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/249https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The «in vitro method» for expression of toxin-production by phenotypically non-toxigenic strains of C. diphtheriae containing the “silent” toxin gene has been developed. The method can be characterised as rapid, economical, not demanding use of experimental animals, available to practical and scientific laboratories. Optimal conditions using this method were defined: nutrient mediums, frequency rate of passages, which provided restoration of toxin production. This method allowed to restore toxin-production in 10 out of 18 tested strains of C. diphtheriae with the “silent” toxin gene. Moreover, there was an increase of toxin roduction of by C. ulcerans and C. diphtheriae var. intermedius to the level allowing to detect toxin in the standard tests. The phenotype of a toxin-producing was defined by the Elek-test and ICS (immunechromatography set).S. A. GabrielyanSankt-Peterburg : NIIÈM imeni Pasteraarticlediphtheritic toxin«silent» toxin generestoration of toxin-producingInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 4, Iss 4, Pp 375-380 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	diphtheritic toxin «silent» toxin gene restoration of toxin-producing Infectious and parasitic diseases RC109-216
spellingShingle	diphtheritic toxin «silent» toxin gene restoration of toxin-producing Infectious and parasitic diseases RC109-216 S. A. Gabrielyan IN VITRO METHOD FOR EXPRESSION OF TOXIN PRODUCTION IN NON-TOXIGENIC CORYNEBACTERIUM SPP. WITH “SILENT” TOX-GENE
description	The «in vitro method» for expression of toxin-production by phenotypically non-toxigenic strains of C. diphtheriae containing the “silent” toxin gene has been developed. The method can be characterised as rapid, economical, not demanding use of experimental animals, available to practical and scientific laboratories. Optimal conditions using this method were defined: nutrient mediums, frequency rate of passages, which provided restoration of toxin production. This method allowed to restore toxin-production in 10 out of 18 tested strains of C. diphtheriae with the “silent” toxin gene. Moreover, there was an increase of toxin roduction of by C. ulcerans and C. diphtheriae var. intermedius to the level allowing to detect toxin in the standard tests. The phenotype of a toxin-producing was defined by the Elek-test and ICS (immunechromatography set).
format	article
author	S. A. Gabrielyan
author_facet	S. A. Gabrielyan
author_sort	S. A. Gabrielyan
title	IN VITRO METHOD FOR EXPRESSION OF TOXIN PRODUCTION IN NON-TOXIGENIC CORYNEBACTERIUM SPP. WITH “SILENT” TOX-GENE
title_short	IN VITRO METHOD FOR EXPRESSION OF TOXIN PRODUCTION IN NON-TOXIGENIC CORYNEBACTERIUM SPP. WITH “SILENT” TOX-GENE
title_full	IN VITRO METHOD FOR EXPRESSION OF TOXIN PRODUCTION IN NON-TOXIGENIC CORYNEBACTERIUM SPP. WITH “SILENT” TOX-GENE
title_fullStr	IN VITRO METHOD FOR EXPRESSION OF TOXIN PRODUCTION IN NON-TOXIGENIC CORYNEBACTERIUM SPP. WITH “SILENT” TOX-GENE
title_full_unstemmed	IN VITRO METHOD FOR EXPRESSION OF TOXIN PRODUCTION IN NON-TOXIGENIC CORYNEBACTERIUM SPP. WITH “SILENT” TOX-GENE
title_sort	in vitro method for expression of toxin production in non-toxigenic corynebacterium spp. with “silent” tox-gene
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2015
url	https://doaj.org/article/7f64e8c2aff54a1ca082d46e16880dd0
work_keys_str_mv	AT sagabrielyan invitromethodforexpressionoftoxinproductioninnontoxigeniccorynebacteriumsppwithsilenttoxgene
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IN VITRO METHOD FOR EXPRESSION OF TOXIN PRODUCTION IN NON-TOXIGENIC CORYNEBACTERIUM SPP. WITH “SILENT” TOX-GENE

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