MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.
Hyperglycemia is generally considered to be an important cause of diabetic retinopathy (DR). The aim of the present study was to investigate the role of miR-5195-3p in high glucose (HG)-induced human retinal pigment epithelial ARPE-19 cell injury. Here, we first found that the expression level of mi...
Guardado en:
Autores principales: | , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/7f667b416c494926a4f7ffdcb856bd21 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:7f667b416c494926a4f7ffdcb856bd21 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:7f667b416c494926a4f7ffdcb856bd212021-12-02T20:12:44ZMicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.1932-620310.1371/journal.pone.0260071https://doaj.org/article/7f667b416c494926a4f7ffdcb856bd212021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0260071https://doaj.org/toc/1932-6203Hyperglycemia is generally considered to be an important cause of diabetic retinopathy (DR). The aim of the present study was to investigate the role of miR-5195-3p in high glucose (HG)-induced human retinal pigment epithelial ARPE-19 cell injury. Here, we first found that the expression level of miR-5195-3p was significantly downregulated in HG-stimulated ARPE-19 cells using reverse transcription quantitative PCR. Overexpression of miR-5195-3p attenuated the impaired cell viability, increased apoptosis and pro-inflammatory cytokines secretion in ARPE-19 cells under HG condition using CCK-8 assay, flow cytometry and ELISA assay, respectively. Luciferase reporter assay showed that miR-5195-3p could specifically bind to the 3'UTR of glia maturation factor-β (GMFB). GMFB overexpression reversed, while knockdown enhanced the protective effects of miR-5195-3p overexpression against HG-induced ARPE-19 cell injury. In summary, miR-5195-3p targeting GMFB might be a potential therapeutic target for DR.Jingjing LiuYongsheng HouLili LinNannan YuYanyan ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0260071 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Jingjing Liu Yongsheng Hou Lili Lin Nannan Yu Yanyan Zhang MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB. |
description |
Hyperglycemia is generally considered to be an important cause of diabetic retinopathy (DR). The aim of the present study was to investigate the role of miR-5195-3p in high glucose (HG)-induced human retinal pigment epithelial ARPE-19 cell injury. Here, we first found that the expression level of miR-5195-3p was significantly downregulated in HG-stimulated ARPE-19 cells using reverse transcription quantitative PCR. Overexpression of miR-5195-3p attenuated the impaired cell viability, increased apoptosis and pro-inflammatory cytokines secretion in ARPE-19 cells under HG condition using CCK-8 assay, flow cytometry and ELISA assay, respectively. Luciferase reporter assay showed that miR-5195-3p could specifically bind to the 3'UTR of glia maturation factor-β (GMFB). GMFB overexpression reversed, while knockdown enhanced the protective effects of miR-5195-3p overexpression against HG-induced ARPE-19 cell injury. In summary, miR-5195-3p targeting GMFB might be a potential therapeutic target for DR. |
format |
article |
author |
Jingjing Liu Yongsheng Hou Lili Lin Nannan Yu Yanyan Zhang |
author_facet |
Jingjing Liu Yongsheng Hou Lili Lin Nannan Yu Yanyan Zhang |
author_sort |
Jingjing Liu |
title |
MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB. |
title_short |
MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB. |
title_full |
MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB. |
title_fullStr |
MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB. |
title_full_unstemmed |
MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB. |
title_sort |
microrna-5195-3p alleviates high glucose‑induced injury in human arpe-19 cells by targeting gmfb. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/7f667b416c494926a4f7ffdcb856bd21 |
work_keys_str_mv |
AT jingjingliu microrna51953palleviateshighglucoseinducedinjuryinhumanarpe19cellsbytargetinggmfb AT yongshenghou microrna51953palleviateshighglucoseinducedinjuryinhumanarpe19cellsbytargetinggmfb AT lililin microrna51953palleviateshighglucoseinducedinjuryinhumanarpe19cellsbytargetinggmfb AT nannanyu microrna51953palleviateshighglucoseinducedinjuryinhumanarpe19cellsbytargetinggmfb AT yanyanzhang microrna51953palleviateshighglucoseinducedinjuryinhumanarpe19cellsbytargetinggmfb |
_version_ |
1718374834522554368 |