MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.

Hyperglycemia is generally considered to be an important cause of diabetic retinopathy (DR). The aim of the present study was to investigate the role of miR-5195-3p in high glucose (HG)-induced human retinal pigment epithelial ARPE-19 cell injury. Here, we first found that the expression level of mi...

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Autores principales: Jingjing Liu, Yongsheng Hou, Lili Lin, Nannan Yu, Yanyan Zhang
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Publicado: Public Library of Science (PLoS) 2021
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Acceso en línea:https://doaj.org/article/7f667b416c494926a4f7ffdcb856bd21
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spelling oai:doaj.org-article:7f667b416c494926a4f7ffdcb856bd212021-12-02T20:12:44ZMicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.1932-620310.1371/journal.pone.0260071https://doaj.org/article/7f667b416c494926a4f7ffdcb856bd212021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0260071https://doaj.org/toc/1932-6203Hyperglycemia is generally considered to be an important cause of diabetic retinopathy (DR). The aim of the present study was to investigate the role of miR-5195-3p in high glucose (HG)-induced human retinal pigment epithelial ARPE-19 cell injury. Here, we first found that the expression level of miR-5195-3p was significantly downregulated in HG-stimulated ARPE-19 cells using reverse transcription quantitative PCR. Overexpression of miR-5195-3p attenuated the impaired cell viability, increased apoptosis and pro-inflammatory cytokines secretion in ARPE-19 cells under HG condition using CCK-8 assay, flow cytometry and ELISA assay, respectively. Luciferase reporter assay showed that miR-5195-3p could specifically bind to the 3'UTR of glia maturation factor-β (GMFB). GMFB overexpression reversed, while knockdown enhanced the protective effects of miR-5195-3p overexpression against HG-induced ARPE-19 cell injury. In summary, miR-5195-3p targeting GMFB might be a potential therapeutic target for DR.Jingjing LiuYongsheng HouLili LinNannan YuYanyan ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0260071 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jingjing Liu
Yongsheng Hou
Lili Lin
Nannan Yu
Yanyan Zhang
MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.
description Hyperglycemia is generally considered to be an important cause of diabetic retinopathy (DR). The aim of the present study was to investigate the role of miR-5195-3p in high glucose (HG)-induced human retinal pigment epithelial ARPE-19 cell injury. Here, we first found that the expression level of miR-5195-3p was significantly downregulated in HG-stimulated ARPE-19 cells using reverse transcription quantitative PCR. Overexpression of miR-5195-3p attenuated the impaired cell viability, increased apoptosis and pro-inflammatory cytokines secretion in ARPE-19 cells under HG condition using CCK-8 assay, flow cytometry and ELISA assay, respectively. Luciferase reporter assay showed that miR-5195-3p could specifically bind to the 3'UTR of glia maturation factor-β (GMFB). GMFB overexpression reversed, while knockdown enhanced the protective effects of miR-5195-3p overexpression against HG-induced ARPE-19 cell injury. In summary, miR-5195-3p targeting GMFB might be a potential therapeutic target for DR.
format article
author Jingjing Liu
Yongsheng Hou
Lili Lin
Nannan Yu
Yanyan Zhang
author_facet Jingjing Liu
Yongsheng Hou
Lili Lin
Nannan Yu
Yanyan Zhang
author_sort Jingjing Liu
title MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.
title_short MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.
title_full MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.
title_fullStr MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.
title_full_unstemmed MicroRNA-5195-3p alleviates high glucose‑induced injury in human ARPE-19 cells by targeting GMFB.
title_sort microrna-5195-3p alleviates high glucose‑induced injury in human arpe-19 cells by targeting gmfb.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/7f667b416c494926a4f7ffdcb856bd21
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AT yongshenghou microrna51953palleviateshighglucoseinducedinjuryinhumanarpe19cellsbytargetinggmfb
AT lililin microrna51953palleviateshighglucoseinducedinjuryinhumanarpe19cellsbytargetinggmfb
AT nannanyu microrna51953palleviateshighglucoseinducedinjuryinhumanarpe19cellsbytargetinggmfb
AT yanyanzhang microrna51953palleviateshighglucoseinducedinjuryinhumanarpe19cellsbytargetinggmfb
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