Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor

Abstract The cervix is responsible for maintaining pregnancy, and its timely remodeling is essential for the proper delivery of a baby. Cervical insufficiency, or “weakness”, may lead to preterm birth, which causes infant morbidities and mortalities worldwide. We used a mouse model of pregnancy and...

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Autores principales:	Antara Chatterjee, Rojan Saghian, Anna Dorogin, Lindsay S. Cahill, John G. Sled, Stephen Lye, Oksana Shynlova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f7f92da7b084b499d328e58cdfe9ed9
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spelling	oai:doaj.org-article:7f7f92da7b084b499d328e58cdfe9ed92021-12-02T15:53:02ZCombination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor10.1038/s41598-021-84036-92045-2322https://doaj.org/article/7f7f92da7b084b499d328e58cdfe9ed92021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84036-9https://doaj.org/toc/2045-2322Abstract The cervix is responsible for maintaining pregnancy, and its timely remodeling is essential for the proper delivery of a baby. Cervical insufficiency, or “weakness”, may lead to preterm birth, which causes infant morbidities and mortalities worldwide. We used a mouse model of pregnancy and term labor, to examine the cervical structure by histology (Masson Trichome and Picrosirius Red staining), immunohistochemistry (Hyaluronic Acid Binding Protein/HABP), and ex-vivo MRI (T2-weighted and diffusion tensor imaging), focusing on two regions of the cervix (i.e., endocervix and ectocervix). Our results show that mouse endocervix has a higher proportion of smooth muscle cells and collagen fibers per area, with more compact tissue structure, than the ectocervix. With advanced gestation, endocervical changes, indicative of impending delivery, are manifested in fewer smooth muscle cells, expansion of the extracellular space, and lower presence of collagen fibers. MRI detected three distinctive zones in pregnant mouse endocervix: (1) inner collagenous layer, (2) middle circular muscular layer, and (3) outer longitudinal muscular layer. Diffusion MRI images detected changes in tissue organization as gestation progressed suggesting the potential application of this technique to non-invasively monitor cervical changes that precede the onset of labor in women at risk for preterm delivery.Antara ChatterjeeRojan SaghianAnna DoroginLindsay S. CahillJohn G. SledStephen LyeOksana ShynlovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antara Chatterjee Rojan Saghian Anna Dorogin Lindsay S. Cahill John G. Sled Stephen Lye Oksana Shynlova Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor
description	Abstract The cervix is responsible for maintaining pregnancy, and its timely remodeling is essential for the proper delivery of a baby. Cervical insufficiency, or “weakness”, may lead to preterm birth, which causes infant morbidities and mortalities worldwide. We used a mouse model of pregnancy and term labor, to examine the cervical structure by histology (Masson Trichome and Picrosirius Red staining), immunohistochemistry (Hyaluronic Acid Binding Protein/HABP), and ex-vivo MRI (T2-weighted and diffusion tensor imaging), focusing on two regions of the cervix (i.e., endocervix and ectocervix). Our results show that mouse endocervix has a higher proportion of smooth muscle cells and collagen fibers per area, with more compact tissue structure, than the ectocervix. With advanced gestation, endocervical changes, indicative of impending delivery, are manifested in fewer smooth muscle cells, expansion of the extracellular space, and lower presence of collagen fibers. MRI detected three distinctive zones in pregnant mouse endocervix: (1) inner collagenous layer, (2) middle circular muscular layer, and (3) outer longitudinal muscular layer. Diffusion MRI images detected changes in tissue organization as gestation progressed suggesting the potential application of this technique to non-invasively monitor cervical changes that precede the onset of labor in women at risk for preterm delivery.
format	article
author	Antara Chatterjee Rojan Saghian Anna Dorogin Lindsay S. Cahill John G. Sled Stephen Lye Oksana Shynlova
author_facet	Antara Chatterjee Rojan Saghian Anna Dorogin Lindsay S. Cahill John G. Sled Stephen Lye Oksana Shynlova
author_sort	Antara Chatterjee
title	Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor
title_short	Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor
title_full	Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor
title_fullStr	Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor
title_full_unstemmed	Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor
title_sort	combination of histochemical analyses and micro-mri reveals regional changes of the murine cervix in preparation for labor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7f7f92da7b084b499d328e58cdfe9ed9
work_keys_str_mv	AT antarachatterjee combinationofhistochemicalanalysesandmicromrirevealsregionalchangesofthemurinecervixinpreparationforlabor AT rojansaghian combinationofhistochemicalanalysesandmicromrirevealsregionalchangesofthemurinecervixinpreparationforlabor AT annadorogin combinationofhistochemicalanalysesandmicromrirevealsregionalchangesofthemurinecervixinpreparationforlabor AT lindsayscahill combinationofhistochemicalanalysesandmicromrirevealsregionalchangesofthemurinecervixinpreparationforlabor AT johngsled combinationofhistochemicalanalysesandmicromrirevealsregionalchangesofthemurinecervixinpreparationforlabor AT stephenlye combinationofhistochemicalanalysesandmicromrirevealsregionalchangesofthemurinecervixinpreparationforlabor AT oksanashynlova combinationofhistochemicalanalysesandmicromrirevealsregionalchangesofthemurinecervixinpreparationforlabor
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Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor

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