Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice

Jun Zheng Goh,1 Sook Nai Tang,1 Hoe Siong Chiong,1,2 Yoke Keong Yong,3 Ahmad Zuraini,1 Muhammad Nazrul Hakim1,4 1Department of Biomedical Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2InQpharm Group, Kuala Lumpur, Malaysia; 3Department of Human Anatomy, 4Halal Product Research...

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Autores principales:	Goh JZ, Tang SN, Chiong HS, Yong YK, Zuraini A, Hakim MN
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
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spelling	oai:doaj.org-article:7f810d8065164acc89bed0fe785a63eb2021-12-02T05:39:45ZEvaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice1178-2013https://doaj.org/article/7f810d8065164acc89bed0fe785a63eb2014-12-01T00:00:00Zhttp://www.dovepress.com/evaluation-of-antinociceptive-activity-of-nanoliposome-encapsulated-an-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Jun Zheng Goh,1 Sook Nai Tang,1 Hoe Siong Chiong,1,2 Yoke Keong Yong,3 Ahmad Zuraini,1 Muhammad Nazrul Hakim1,4 1Department of Biomedical Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2InQpharm Group, Kuala Lumpur, Malaysia; 3Department of Human Anatomy, 4Halal Product Research Institute, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Abstract: Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) that exhibits anti-inflammatory, antinociceptive, and antipyretic activities. Liposomes have been shown to improve the therapeutic efficacy of encapsulated drugs. The present study was conducted to compare the antinociceptive properties between liposome-encapsulated and free-form diclofenac in vivo via different nociceptive assay models. Liposome-encapsulated diclofenac was prepared using the commercialized proliposome method. Antinociceptive effects of liposome-encapsulated and free-form diclofenac were evaluated using formalin test, acetic acid-induced abdominal writhing test, Randall–Selitto paw pressure test, and plantar test. The results of the writhing test showed a significant reduction of abdominal constriction in all treatment groups in a dose-dependent manner. The 20 mg/kg liposome-encapsulated diclofenac demonstrated the highest antinociceptive effect at 78.97% compared with 55.89% in the free-form group at equivalent dosage. Both liposome-encapsulated and free-form diclofenac produced significant results in the late phase of formalin assay at a dose of 20 mg/kg, with antinociception percentages of 78.84% and 60.71%, respectively. Significant results of antinociception were also observed in both hyperalgesia assays. For Randall–Sellito assay, the highest antinociception effect of 71.38% was achieved with 20 mg/kg liposome-encapsulated diclofenac, while the lowest antinociceptive effect of 17.32% was recorded with 0 mg/kg liposome formulation, whereas in the plantar test, the highest antinociceptive effect was achieved at 56.7% with 20 mg/kg liposome-encapsulated diclofenac, and the lowest effect was shown with 0 mg/kg liposome formulation of 8.89%. The present study suggests that liposome-encapsulated diclofenac exhibits higher antinociceptive efficacy in a dose-dependent manner in comparison with free-form diclofenac. Keywords: diclofenac, liposome, nanoencapsulation, efficacy, antinociceptiveGoh JZTang SNChiong HSYong YKZuraini AHakim MNDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 297-303 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Goh JZ Tang SN Chiong HS Yong YK Zuraini A Hakim MN Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice
description	Jun Zheng Goh,1 Sook Nai Tang,1 Hoe Siong Chiong,1,2 Yoke Keong Yong,3 Ahmad Zuraini,1 Muhammad Nazrul Hakim1,4 1Department of Biomedical Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; 2InQpharm Group, Kuala Lumpur, Malaysia; 3Department of Human Anatomy, 4Halal Product Research Institute, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Abstract: Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) that exhibits anti-inflammatory, antinociceptive, and antipyretic activities. Liposomes have been shown to improve the therapeutic efficacy of encapsulated drugs. The present study was conducted to compare the antinociceptive properties between liposome-encapsulated and free-form diclofenac in vivo via different nociceptive assay models. Liposome-encapsulated diclofenac was prepared using the commercialized proliposome method. Antinociceptive effects of liposome-encapsulated and free-form diclofenac were evaluated using formalin test, acetic acid-induced abdominal writhing test, Randall–Selitto paw pressure test, and plantar test. The results of the writhing test showed a significant reduction of abdominal constriction in all treatment groups in a dose-dependent manner. The 20 mg/kg liposome-encapsulated diclofenac demonstrated the highest antinociceptive effect at 78.97% compared with 55.89% in the free-form group at equivalent dosage. Both liposome-encapsulated and free-form diclofenac produced significant results in the late phase of formalin assay at a dose of 20 mg/kg, with antinociception percentages of 78.84% and 60.71%, respectively. Significant results of antinociception were also observed in both hyperalgesia assays. For Randall–Sellito assay, the highest antinociception effect of 71.38% was achieved with 20 mg/kg liposome-encapsulated diclofenac, while the lowest antinociceptive effect of 17.32% was recorded with 0 mg/kg liposome formulation, whereas in the plantar test, the highest antinociceptive effect was achieved at 56.7% with 20 mg/kg liposome-encapsulated diclofenac, and the lowest effect was shown with 0 mg/kg liposome formulation of 8.89%. The present study suggests that liposome-encapsulated diclofenac exhibits higher antinociceptive efficacy in a dose-dependent manner in comparison with free-form diclofenac. Keywords: diclofenac, liposome, nanoencapsulation, efficacy, antinociceptive
format	article
author	Goh JZ Tang SN Chiong HS Yong YK Zuraini A Hakim MN
author_facet	Goh JZ Tang SN Chiong HS Yong YK Zuraini A Hakim MN
author_sort	Goh JZ
title	Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice
title_short	Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice
title_full	Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice
title_fullStr	Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice
title_full_unstemmed	Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice
title_sort	evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/7f810d8065164acc89bed0fe785a63eb
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Evaluation of antinociceptive activity of nanoliposome-encapsulated and free-form diclofenac in rats and mice

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