Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm

Abstract Evaluation of transcriptome data in combination with QTL information has been applied in many crops to study the expression of genes responsible for specific phenotypes. In oil palm, the mesocarp oil extracted from E. oleifera × E. guineensis interspecific hybrids is known to have lower pal...

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Autores principales:	Ngoot-Chin Ting, Katrina Sherbina, Jia-Shiun Khoo, Katialisa Kamaruddin, Pek-Lan Chan, Kuang-Lim Chan, Mohd Amin Ab Halim, Kandha Sritharan, Zulkifli Yaakub, Sean Mayes, Festo Massawe, Peter L. Chang, Sergey V. Nuzhdin, Ravigadevi Sambanthamurthi, Rajinder Singh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f82132b81d7401081c8d63c587ed9d5
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spelling	oai:doaj.org-article:7f82132b81d7401081c8d63c587ed9d52021-12-02T18:51:06ZExpression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm10.1038/s41598-020-73170-52045-2322https://doaj.org/article/7f82132b81d7401081c8d63c587ed9d52020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73170-5https://doaj.org/toc/2045-2322Abstract Evaluation of transcriptome data in combination with QTL information has been applied in many crops to study the expression of genes responsible for specific phenotypes. In oil palm, the mesocarp oil extracted from E. oleifera × E. guineensis interspecific hybrids is known to have lower palmitic acid (C16:0) content compared to pure African palms. The present study demonstrates the effectiveness of transcriptome data in revealing the expression profiles of genes in the fatty acid (FA) and triacylglycerol (TAG) biosynthesis processes in interspecific hybrids. The transcriptome assembly yielded 43,920 putative genes of which a large proportion were homologous to known genes in the public databases. Most of the genes encoding key enzymes involved in the FA and TAG synthesis pathways were identified. Of these, 27, including two candidate genes located within the QTL associated with C16:0 content, showed differential expression between developmental stages, populations and/or palms with contrasting C16:0 content. Further evaluation using quantitative real-time PCR revealed that differentially expressed patterns are generally consistent with those observed in the transcriptome data. Our results also suggest that different isoforms are likely to be responsible for some of the variation observed in FA composition of interspecific hybrids.Ngoot-Chin TingKatrina SherbinaJia-Shiun KhooKatialisa KamaruddinPek-Lan ChanKuang-Lim ChanMohd Amin Ab HalimKandha SritharanZulkifli YaakubSean MayesFesto MassawePeter L. ChangSergey V. NuzhdinRavigadevi SambanthamurthiRajinder SinghNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ngoot-Chin Ting Katrina Sherbina Jia-Shiun Khoo Katialisa Kamaruddin Pek-Lan Chan Kuang-Lim Chan Mohd Amin Ab Halim Kandha Sritharan Zulkifli Yaakub Sean Mayes Festo Massawe Peter L. Chang Sergey V. Nuzhdin Ravigadevi Sambanthamurthi Rajinder Singh Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm
description	Abstract Evaluation of transcriptome data in combination with QTL information has been applied in many crops to study the expression of genes responsible for specific phenotypes. In oil palm, the mesocarp oil extracted from E. oleifera × E. guineensis interspecific hybrids is known to have lower palmitic acid (C16:0) content compared to pure African palms. The present study demonstrates the effectiveness of transcriptome data in revealing the expression profiles of genes in the fatty acid (FA) and triacylglycerol (TAG) biosynthesis processes in interspecific hybrids. The transcriptome assembly yielded 43,920 putative genes of which a large proportion were homologous to known genes in the public databases. Most of the genes encoding key enzymes involved in the FA and TAG synthesis pathways were identified. Of these, 27, including two candidate genes located within the QTL associated with C16:0 content, showed differential expression between developmental stages, populations and/or palms with contrasting C16:0 content. Further evaluation using quantitative real-time PCR revealed that differentially expressed patterns are generally consistent with those observed in the transcriptome data. Our results also suggest that different isoforms are likely to be responsible for some of the variation observed in FA composition of interspecific hybrids.
format	article
author	Ngoot-Chin Ting Katrina Sherbina Jia-Shiun Khoo Katialisa Kamaruddin Pek-Lan Chan Kuang-Lim Chan Mohd Amin Ab Halim Kandha Sritharan Zulkifli Yaakub Sean Mayes Festo Massawe Peter L. Chang Sergey V. Nuzhdin Ravigadevi Sambanthamurthi Rajinder Singh
author_facet	Ngoot-Chin Ting Katrina Sherbina Jia-Shiun Khoo Katialisa Kamaruddin Pek-Lan Chan Kuang-Lim Chan Mohd Amin Ab Halim Kandha Sritharan Zulkifli Yaakub Sean Mayes Festo Massawe Peter L. Chang Sergey V. Nuzhdin Ravigadevi Sambanthamurthi Rajinder Singh
author_sort	Ngoot-Chin Ting
title	Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm
title_short	Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm
title_full	Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm
title_fullStr	Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm
title_full_unstemmed	Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm
title_sort	expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7f82132b81d7401081c8d63c587ed9d5
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Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm

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