Diversification of specificity after maturation of the antibody response to the HIV gp41 epitope ELDKWA.

During maturing antibody responses the increase in affinity for target antigens is achieved by genetic diversification of antibody genes followed by selection for improved binding. The effect this process has on the specificity of antibody for variants of the antigen is not well-defined, despite the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Henry N White, Qing-Hai Meng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f865b1024e844dfb7e6b00f9a0eac79
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7f865b1024e844dfb7e6b00f9a0eac79
record_format	dspace
spelling	oai:doaj.org-article:7f865b1024e844dfb7e6b00f9a0eac792021-11-18T07:28:20ZDiversification of specificity after maturation of the antibody response to the HIV gp41 epitope ELDKWA.1932-620310.1371/journal.pone.0031555https://doaj.org/article/7f865b1024e844dfb7e6b00f9a0eac792012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22348106/?tool=EBIhttps://doaj.org/toc/1932-6203During maturing antibody responses the increase in affinity for target antigens is achieved by genetic diversification of antibody genes followed by selection for improved binding. The effect this process has on the specificity of antibody for variants of the antigen is not well-defined, despite the potential role of antibody diversification in generating enhanced protection against pathogen escape mutants, or novel specificities after vaccination. To investigate this, a library of single amino-acid substitution epitope variants has been screened with serum obtained at different time-points after immunization of mice with the HIV gp41 peptide epitope ELDKWA. The serum IgG response is shown to mature and increase affinity for ELDKWA, and the titre and affinity of IgG against most epitope variants tested increases. Furthermore there is a bias towards high affinity serum IgG binding to variant epitopes with conservative substitutions, although underlying this trend there is also significant binding to many epitopes with non-conservative substitutions. Thus, maturation of the antibody response to a single epitope results in a broadening of the high-affinity response toward variant epitopes. This implies that many pathogen epitope escape variants that could manifest as single amino-acid substitutions would not emerge by escaping immune surveillance.Henry N WhiteQing-Hai MengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31555 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Henry N White Qing-Hai Meng Diversification of specificity after maturation of the antibody response to the HIV gp41 epitope ELDKWA.
description	During maturing antibody responses the increase in affinity for target antigens is achieved by genetic diversification of antibody genes followed by selection for improved binding. The effect this process has on the specificity of antibody for variants of the antigen is not well-defined, despite the potential role of antibody diversification in generating enhanced protection against pathogen escape mutants, or novel specificities after vaccination. To investigate this, a library of single amino-acid substitution epitope variants has been screened with serum obtained at different time-points after immunization of mice with the HIV gp41 peptide epitope ELDKWA. The serum IgG response is shown to mature and increase affinity for ELDKWA, and the titre and affinity of IgG against most epitope variants tested increases. Furthermore there is a bias towards high affinity serum IgG binding to variant epitopes with conservative substitutions, although underlying this trend there is also significant binding to many epitopes with non-conservative substitutions. Thus, maturation of the antibody response to a single epitope results in a broadening of the high-affinity response toward variant epitopes. This implies that many pathogen epitope escape variants that could manifest as single amino-acid substitutions would not emerge by escaping immune surveillance.
format	article
author	Henry N White Qing-Hai Meng
author_facet	Henry N White Qing-Hai Meng
author_sort	Henry N White
title	Diversification of specificity after maturation of the antibody response to the HIV gp41 epitope ELDKWA.
title_short	Diversification of specificity after maturation of the antibody response to the HIV gp41 epitope ELDKWA.
title_full	Diversification of specificity after maturation of the antibody response to the HIV gp41 epitope ELDKWA.
title_fullStr	Diversification of specificity after maturation of the antibody response to the HIV gp41 epitope ELDKWA.
title_full_unstemmed	Diversification of specificity after maturation of the antibody response to the HIV gp41 epitope ELDKWA.
title_sort	diversification of specificity after maturation of the antibody response to the hiv gp41 epitope eldkwa.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7f865b1024e844dfb7e6b00f9a0eac79
work_keys_str_mv	AT henrynwhite diversificationofspecificityaftermaturationoftheantibodyresponsetothehivgp41epitopeeldkwa AT qinghaimeng diversificationofspecificityaftermaturationoftheantibodyresponsetothehivgp41epitopeeldkwa
_version_	1718423420113256448

Diversification of specificity after maturation of the antibody response to the HIV gp41 epitope ELDKWA.

Ejemplares similares