The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)
Abstract The purpose of the present study was to evaluate de influence of protein–sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive p...
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Nature Portfolio
2021
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oai:doaj.org-article:7f8ac6ecf06b4234a51c71a072703a7d2021-12-02T16:07:03ZThe influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP)10.1038/s41598-021-92522-32045-2322https://doaj.org/article/7f8ac6ecf06b4234a51c71a072703a7d2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92522-3https://doaj.org/toc/2045-2322Abstract The purpose of the present study was to evaluate de influence of protein–sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive protein is a plasmatic protein used as a biomarker for the diagnosis of a series of health problems such as ulcerative colitis, cardiovascular diseases, metabolic syndrome, due to its essential role in the evolution of chronic inflammation. The sugar–protein interaction was investigated using steady state and time resolved fluorescence. The results revealed that there are more than two classes of tryptophan, with different degree of accessibility for the quencher molecule. Our study also revealed that sugar–protein complexes have superior thermostability, especially after gamma irradiation at 2 kGy, the protein being stable and functional even after 22 days exposure to 40 °C.Andreea Lorena MateescuNicolae-Bogdan MincuSilvana VasilcaRoxana ApetreiDiana StanBogdan ZorilăDana StanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q Andreea Lorena Mateescu Nicolae-Bogdan Mincu Silvana Vasilca Roxana Apetrei Diana Stan Bogdan Zorilă Dana Stan The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP) |
| description |
Abstract The purpose of the present study was to evaluate de influence of protein–sugar complexation on the stability and functionality of C-reactive protein, after exposure to constant high temperatures, in order to develop highly stable positive controls for in-vitro diagnostic tests. C-reactive protein is a plasmatic protein used as a biomarker for the diagnosis of a series of health problems such as ulcerative colitis, cardiovascular diseases, metabolic syndrome, due to its essential role in the evolution of chronic inflammation. The sugar–protein interaction was investigated using steady state and time resolved fluorescence. The results revealed that there are more than two classes of tryptophan, with different degree of accessibility for the quencher molecule. Our study also revealed that sugar–protein complexes have superior thermostability, especially after gamma irradiation at 2 kGy, the protein being stable and functional even after 22 days exposure to 40 °C. |
| format |
article |
| author |
Andreea Lorena Mateescu Nicolae-Bogdan Mincu Silvana Vasilca Roxana Apetrei Diana Stan Bogdan Zorilă Dana Stan |
| author_facet |
Andreea Lorena Mateescu Nicolae-Bogdan Mincu Silvana Vasilca Roxana Apetrei Diana Stan Bogdan Zorilă Dana Stan |
| author_sort |
Andreea Lorena Mateescu |
| title |
The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP) |
| title_short |
The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP) |
| title_full |
The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP) |
| title_fullStr |
The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP) |
| title_full_unstemmed |
The influence of sugar–protein complexes on the thermostability of C-reactive protein (CRP) |
| title_sort |
influence of sugar–protein complexes on the thermostability of c-reactive protein (crp) |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/7f8ac6ecf06b4234a51c71a072703a7d |
| work_keys_str_mv |
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