DEspRhigh neutrophils are associated with critical illness in COVID-19

Abstract SARS-CoV-2 infection results in a spectrum of outcomes from no symptoms to widely varying degrees of illness to death. A better understanding of the immune response to SARS-CoV-2 infection and subsequent, often excessive, inflammation may inform treatment decisions and reveal opportunities...

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Autores principales:	Joanne T. deKay, Ivette F. Emery, Jonathan Rud, Ashley Eldridge, Christine Lord, David J. Gagnon, Teresa L. May, Victoria L. M. Herrera, Nelson Ruiz-Opazo, Richard R. Riker, Douglas B. Sawyer, Sergey Ryzhov, David B. Seder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f8cafdfa06e450c8c3f9913a5c5c788
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spelling	oai:doaj.org-article:7f8cafdfa06e450c8c3f9913a5c5c7882021-11-21T12:24:06ZDEspRhigh neutrophils are associated with critical illness in COVID-1910.1038/s41598-021-01943-72045-2322https://doaj.org/article/7f8cafdfa06e450c8c3f9913a5c5c7882021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01943-7https://doaj.org/toc/2045-2322Abstract SARS-CoV-2 infection results in a spectrum of outcomes from no symptoms to widely varying degrees of illness to death. A better understanding of the immune response to SARS-CoV-2 infection and subsequent, often excessive, inflammation may inform treatment decisions and reveal opportunities for therapy. We studied immune cell subpopulations and their associations with clinical parameters in a cohort of 26 patients with COVID-19. Following informed consent, we collected blood samples from hospitalized patients with COVID-19 within 72 h of admission. Flow cytometry was used to analyze white blood cell subpopulations. Plasma levels of cytokines and chemokines were measured using ELISA. Neutrophils undergoing neutrophil extracellular traps (NET) formation were evaluated in blood smears. We examined the immunophenotype of patients with COVID-19 in comparison to that of SARS-CoV-2 negative controls. A novel subset of pro-inflammatory neutrophils expressing a high level of dual endothelin-1 and VEGF signal peptide-activated receptor (DEspR) at the cell surface was found to be associated with elevated circulating CCL23, increased NETosis, and critical-severity COVID-19 illness. The potential to target this subpopulation of neutrophils to reduce secondary tissue damage caused by SARS-CoV-2 infection warrants further investigation.Joanne T. deKayIvette F. EmeryJonathan RudAshley EldridgeChristine LordDavid J. GagnonTeresa L. MayVictoria L. M. HerreraNelson Ruiz-OpazoRichard R. RikerDouglas B. SawyerSergey RyzhovDavid B. SederNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joanne T. deKay Ivette F. Emery Jonathan Rud Ashley Eldridge Christine Lord David J. Gagnon Teresa L. May Victoria L. M. Herrera Nelson Ruiz-Opazo Richard R. Riker Douglas B. Sawyer Sergey Ryzhov David B. Seder DEspRhigh neutrophils are associated with critical illness in COVID-19
description	Abstract SARS-CoV-2 infection results in a spectrum of outcomes from no symptoms to widely varying degrees of illness to death. A better understanding of the immune response to SARS-CoV-2 infection and subsequent, often excessive, inflammation may inform treatment decisions and reveal opportunities for therapy. We studied immune cell subpopulations and their associations with clinical parameters in a cohort of 26 patients with COVID-19. Following informed consent, we collected blood samples from hospitalized patients with COVID-19 within 72 h of admission. Flow cytometry was used to analyze white blood cell subpopulations. Plasma levels of cytokines and chemokines were measured using ELISA. Neutrophils undergoing neutrophil extracellular traps (NET) formation were evaluated in blood smears. We examined the immunophenotype of patients with COVID-19 in comparison to that of SARS-CoV-2 negative controls. A novel subset of pro-inflammatory neutrophils expressing a high level of dual endothelin-1 and VEGF signal peptide-activated receptor (DEspR) at the cell surface was found to be associated with elevated circulating CCL23, increased NETosis, and critical-severity COVID-19 illness. The potential to target this subpopulation of neutrophils to reduce secondary tissue damage caused by SARS-CoV-2 infection warrants further investigation.
format	article
author	Joanne T. deKay Ivette F. Emery Jonathan Rud Ashley Eldridge Christine Lord David J. Gagnon Teresa L. May Victoria L. M. Herrera Nelson Ruiz-Opazo Richard R. Riker Douglas B. Sawyer Sergey Ryzhov David B. Seder
author_facet	Joanne T. deKay Ivette F. Emery Jonathan Rud Ashley Eldridge Christine Lord David J. Gagnon Teresa L. May Victoria L. M. Herrera Nelson Ruiz-Opazo Richard R. Riker Douglas B. Sawyer Sergey Ryzhov David B. Seder
author_sort	Joanne T. deKay
title	DEspRhigh neutrophils are associated with critical illness in COVID-19
title_short	DEspRhigh neutrophils are associated with critical illness in COVID-19
title_full	DEspRhigh neutrophils are associated with critical illness in COVID-19
title_fullStr	DEspRhigh neutrophils are associated with critical illness in COVID-19
title_full_unstemmed	DEspRhigh neutrophils are associated with critical illness in COVID-19
title_sort	desprhigh neutrophils are associated with critical illness in covid-19
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7f8cafdfa06e450c8c3f9913a5c5c788
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DEspRhigh neutrophils are associated with critical illness in COVID-19

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