Differential proteomics of tobacco seedling roots at high and low potassium concentrations

Abstract The effects of high potassium and normal potassium treatments on protein expression in roots of flue-cured tobacco plant HKDN-5 at the seedling stage were analyzed by an unlabeled protein quantification technique. The results showed that 555 proteins were differentially expressed (245 prote...

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Autores principales:	Lin-jian Dai, Yu-kun Liu, Chong-wen Zhu, Jun Zhong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f92c0af3c544eeab98a6079b703814d
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spelling	oai:doaj.org-article:7f92c0af3c544eeab98a6079b703814d2021-12-02T14:53:42ZDifferential proteomics of tobacco seedling roots at high and low potassium concentrations10.1038/s41598-021-88689-42045-2322https://doaj.org/article/7f92c0af3c544eeab98a6079b703814d2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88689-4https://doaj.org/toc/2045-2322Abstract The effects of high potassium and normal potassium treatments on protein expression in roots of flue-cured tobacco plant HKDN-5 at the seedling stage were analyzed by an unlabeled protein quantification technique. The results showed that 555 proteins were differentially expressed (245 proteins were down-regulated and 310 proteins were up-regulated) in high potassium treatment compared with normal potassium treatment. Differentially expressed proteins were involved in 96 metabolic pathways (42 metabolic pathways, 21 synthetic pathways as well as catabolic pathways, including fatty acid metabolism, phenylpropane biosynthesis, ketone body synthesis and degradation, and butyric acid metabolism. Root processing of high potassium concentrations leads to increases in the synthesis of peroxidase, superoxide dismutase and acyl-coenzyme-A synthetase. Additional proteomic differences observed in tobacco roots grown in high potassium include proteins involved with genetic information processing as well as environmental sensing. Examples include RNA helicase, ABC transporters and large subunit GTPases. These up-regulated differentially expressed proteins function mainly in protein translation, ribosome structure and protein synthesis. This indicates that under high potassium treatment, root protein synthetic processes are accelerated and substance metabolism pathways are enhanced; thus, providing the material and energetic basis for root growth.Lin-jian DaiYu-kun LiuChong-wen ZhuJun ZhongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lin-jian Dai Yu-kun Liu Chong-wen Zhu Jun Zhong Differential proteomics of tobacco seedling roots at high and low potassium concentrations
description	Abstract The effects of high potassium and normal potassium treatments on protein expression in roots of flue-cured tobacco plant HKDN-5 at the seedling stage were analyzed by an unlabeled protein quantification technique. The results showed that 555 proteins were differentially expressed (245 proteins were down-regulated and 310 proteins were up-regulated) in high potassium treatment compared with normal potassium treatment. Differentially expressed proteins were involved in 96 metabolic pathways (42 metabolic pathways, 21 synthetic pathways as well as catabolic pathways, including fatty acid metabolism, phenylpropane biosynthesis, ketone body synthesis and degradation, and butyric acid metabolism. Root processing of high potassium concentrations leads to increases in the synthesis of peroxidase, superoxide dismutase and acyl-coenzyme-A synthetase. Additional proteomic differences observed in tobacco roots grown in high potassium include proteins involved with genetic information processing as well as environmental sensing. Examples include RNA helicase, ABC transporters and large subunit GTPases. These up-regulated differentially expressed proteins function mainly in protein translation, ribosome structure and protein synthesis. This indicates that under high potassium treatment, root protein synthetic processes are accelerated and substance metabolism pathways are enhanced; thus, providing the material and energetic basis for root growth.
format	article
author	Lin-jian Dai Yu-kun Liu Chong-wen Zhu Jun Zhong
author_facet	Lin-jian Dai Yu-kun Liu Chong-wen Zhu Jun Zhong
author_sort	Lin-jian Dai
title	Differential proteomics of tobacco seedling roots at high and low potassium concentrations
title_short	Differential proteomics of tobacco seedling roots at high and low potassium concentrations
title_full	Differential proteomics of tobacco seedling roots at high and low potassium concentrations
title_fullStr	Differential proteomics of tobacco seedling roots at high and low potassium concentrations
title_full_unstemmed	Differential proteomics of tobacco seedling roots at high and low potassium concentrations
title_sort	differential proteomics of tobacco seedling roots at high and low potassium concentrations
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7f92c0af3c544eeab98a6079b703814d
work_keys_str_mv	AT linjiandai differentialproteomicsoftobaccoseedlingrootsathighandlowpotassiumconcentrations AT yukunliu differentialproteomicsoftobaccoseedlingrootsathighandlowpotassiumconcentrations AT chongwenzhu differentialproteomicsoftobaccoseedlingrootsathighandlowpotassiumconcentrations AT junzhong differentialproteomicsoftobaccoseedlingrootsathighandlowpotassiumconcentrations
_version_	1718389393538940928

Differential proteomics of tobacco seedling roots at high and low potassium concentrations

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