An Infection-Based Murine Model for Papillomavirus-Associated Head and Neck Cancer

ABSTRACT Human papillomavirus (HPV) is the most common sexually transmitted pathogen, and high-risk HPVs contribute to 5% of human cancers, including 25% of head and neck squamous cell carcinomas (HNSCCs). Despite the significant role played by HPVs in HNSCC, there is currently no available in vivo...

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Autores principales:	Tao Wei, Darya Buehler, Ella Ward-Shaw, Paul F. Lambert
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	MmuPV1 head and neck cancer papillomavirus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7f934801cf5f4637a8bd8f8a860c6a37
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spelling	oai:doaj.org-article:7f934801cf5f4637a8bd8f8a860c6a372021-11-15T15:56:46ZAn Infection-Based Murine Model for Papillomavirus-Associated Head and Neck Cancer10.1128/mBio.00908-202150-7511https://doaj.org/article/7f934801cf5f4637a8bd8f8a860c6a372020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00908-20https://doaj.org/toc/2150-7511ABSTRACT Human papillomavirus (HPV) is the most common sexually transmitted pathogen, and high-risk HPVs contribute to 5% of human cancers, including 25% of head and neck squamous cell carcinomas (HNSCCs). Despite the significant role played by HPVs in HNSCC, there is currently no available in vivo system to model the process from papillomavirus infection to virus-induced HNSCC. In this paper, we describe an infection-based HNSCC model, utilizing a mouse papillomavirus (MmuPV1), which naturally infects laboratory mice. Infections of the tongue epithelium of two immunodeficient strains with MmuPV1 caused high-grade squamous dysplasia with early signs of invasive carcinoma over the course of 4 months. When combined with the oral carcinogen 4-nitroquinoline-1-oxide (4NQO), MmuPV1 caused invasive squamous cell carcinoma (SCC) on the tongue of both immunodeficient and immunocompetent mice. These tumors expressed markers of papillomavirus infection and HPV-associated carcinogenesis. This novel preclinical model provides a valuable new means to study how natural papillomavirus infections contribute to HNSCC. IMPORTANCE The species specificity of papillomavirus has limited the development of an infection-based animal model to study HPV-associated head and neck carcinogenesis. Our study presents a novel in vivo model using the mouse papillomavirus MmuPV1 to study papillomavirus-associated head and neck cancer. In our model, MmuPV1 infects and causes lesions in both immunodeficient and genetically immunocompetent strains of mice. These virally induced lesions carry features associated with both HPV infections and HPV-associated carcinogenesis. Combined with previously identified cancer cofactors, MmuPV1 causes invasive squamous cell carcinomas in mice. This model provides opportunities for basic and translational studies of papillomavirus infection-based head and neck disease.Tao WeiDarya BuehlerElla Ward-ShawPaul F. LambertAmerican Society for MicrobiologyarticleMmuPV1head and neck cancerpapillomavirusMicrobiologyQR1-502ENmBio, Vol 11, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	MmuPV1 head and neck cancer papillomavirus Microbiology QR1-502
spellingShingle	MmuPV1 head and neck cancer papillomavirus Microbiology QR1-502 Tao Wei Darya Buehler Ella Ward-Shaw Paul F. Lambert An Infection-Based Murine Model for Papillomavirus-Associated Head and Neck Cancer
description	ABSTRACT Human papillomavirus (HPV) is the most common sexually transmitted pathogen, and high-risk HPVs contribute to 5% of human cancers, including 25% of head and neck squamous cell carcinomas (HNSCCs). Despite the significant role played by HPVs in HNSCC, there is currently no available in vivo system to model the process from papillomavirus infection to virus-induced HNSCC. In this paper, we describe an infection-based HNSCC model, utilizing a mouse papillomavirus (MmuPV1), which naturally infects laboratory mice. Infections of the tongue epithelium of two immunodeficient strains with MmuPV1 caused high-grade squamous dysplasia with early signs of invasive carcinoma over the course of 4 months. When combined with the oral carcinogen 4-nitroquinoline-1-oxide (4NQO), MmuPV1 caused invasive squamous cell carcinoma (SCC) on the tongue of both immunodeficient and immunocompetent mice. These tumors expressed markers of papillomavirus infection and HPV-associated carcinogenesis. This novel preclinical model provides a valuable new means to study how natural papillomavirus infections contribute to HNSCC. IMPORTANCE The species specificity of papillomavirus has limited the development of an infection-based animal model to study HPV-associated head and neck carcinogenesis. Our study presents a novel in vivo model using the mouse papillomavirus MmuPV1 to study papillomavirus-associated head and neck cancer. In our model, MmuPV1 infects and causes lesions in both immunodeficient and genetically immunocompetent strains of mice. These virally induced lesions carry features associated with both HPV infections and HPV-associated carcinogenesis. Combined with previously identified cancer cofactors, MmuPV1 causes invasive squamous cell carcinomas in mice. This model provides opportunities for basic and translational studies of papillomavirus infection-based head and neck disease.
format	article
author	Tao Wei Darya Buehler Ella Ward-Shaw Paul F. Lambert
author_facet	Tao Wei Darya Buehler Ella Ward-Shaw Paul F. Lambert
author_sort	Tao Wei
title	An Infection-Based Murine Model for Papillomavirus-Associated Head and Neck Cancer
title_short	An Infection-Based Murine Model for Papillomavirus-Associated Head and Neck Cancer
title_full	An Infection-Based Murine Model for Papillomavirus-Associated Head and Neck Cancer
title_fullStr	An Infection-Based Murine Model for Papillomavirus-Associated Head and Neck Cancer
title_full_unstemmed	An Infection-Based Murine Model for Papillomavirus-Associated Head and Neck Cancer
title_sort	infection-based murine model for papillomavirus-associated head and neck cancer
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/7f934801cf5f4637a8bd8f8a860c6a37
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An Infection-Based Murine Model for Papillomavirus-Associated Head and Neck Cancer

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