Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity

Antioxidant biomaterials have attracted much attention in various biomedical fields because of their effective inhibition and elimination of reactive oxygen species (ROS) in pathological tissues. However, the difficulty in ensuring biocompatibility, biodegradability and bioavailability of antioxidan...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lulu Wang, Xiaokang Zhang, Pingping Xu, Jicheng Yan, Yuzhong Zhang, Hainan Su, Chengjun Sun, Qiang Lu, Weizhi Liu
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Sessile marine organisms Antioxidant biomaterials Water-resistant coatings Redox-responsive hydrogels Photoaging Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7f965126b586488c86a9a1a8cc705775
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7f965126b586488c86a9a1a8cc705775
record_format	dspace
spelling	oai:doaj.org-article:7f965126b586488c86a9a1a8cc7057752021-11-30T04:16:46ZExploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity2452-199X10.1016/j.bioactmat.2021.08.021https://doaj.org/article/7f965126b586488c86a9a1a8cc7057752022-04-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21003984https://doaj.org/toc/2452-199XAntioxidant biomaterials have attracted much attention in various biomedical fields because of their effective inhibition and elimination of reactive oxygen species (ROS) in pathological tissues. However, the difficulty in ensuring biocompatibility, biodegradability and bioavailability of antioxidant materials has limited their further development. Novel bioavailable antioxidant materials that are derived from natural resources are urgently needed. Here, an integrated multi-omics method was applied to fabricate antioxidant biomaterials. A key cysteine-rich thrombospondin-1 type I repeat-like (TSRL) protein was efficiently discovered from among 1262 adhesive components and then used to create a recombinant protein with a yield of 500 mg L−1. The biocompatible TSRL protein was able to self-assemble into either a water-resistant coating through Ca2+-mediated coordination or redox-responsive hydrogels with tunable physical properties. The TSRL-based hydrogels showed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rates than glutathione (GSH) and ascorbic acid (Aa) and protected cells against external oxidative stress significantly more effectively. When topically applied to mice skin, TSRL alleviated epidermal hyperplasia and suppressed the degradation of collagen and elastic fibers caused by ultraviolet radiation B (UVB) irradiation, confirming that it enhanced antioxidant activity in vivo. This is the first study to successfully characterize natural antioxidant biomaterials created from marine invertebrate adhesives, and the findings indicate the excellent prospects of these biomaterials for great applications in tissue regeneration and cosmeceuticals.Lulu WangXiaokang ZhangPingping XuJicheng YanYuzhong ZhangHainan SuChengjun SunQiang LuWeizhi LiuKeAi Communications Co., Ltd.articleSessile marine organismsAntioxidant biomaterialsWater-resistant coatingsRedox-responsive hydrogelsPhotoagingMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 10, Iss , Pp 504-514 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sessile marine organisms Antioxidant biomaterials Water-resistant coatings Redox-responsive hydrogels Photoaging Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Sessile marine organisms Antioxidant biomaterials Water-resistant coatings Redox-responsive hydrogels Photoaging Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Lulu Wang Xiaokang Zhang Pingping Xu Jicheng Yan Yuzhong Zhang Hainan Su Chengjun Sun Qiang Lu Weizhi Liu Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity
description	Antioxidant biomaterials have attracted much attention in various biomedical fields because of their effective inhibition and elimination of reactive oxygen species (ROS) in pathological tissues. However, the difficulty in ensuring biocompatibility, biodegradability and bioavailability of antioxidant materials has limited their further development. Novel bioavailable antioxidant materials that are derived from natural resources are urgently needed. Here, an integrated multi-omics method was applied to fabricate antioxidant biomaterials. A key cysteine-rich thrombospondin-1 type I repeat-like (TSRL) protein was efficiently discovered from among 1262 adhesive components and then used to create a recombinant protein with a yield of 500 mg L−1. The biocompatible TSRL protein was able to self-assemble into either a water-resistant coating through Ca2+-mediated coordination or redox-responsive hydrogels with tunable physical properties. The TSRL-based hydrogels showed stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rates than glutathione (GSH) and ascorbic acid (Aa) and protected cells against external oxidative stress significantly more effectively. When topically applied to mice skin, TSRL alleviated epidermal hyperplasia and suppressed the degradation of collagen and elastic fibers caused by ultraviolet radiation B (UVB) irradiation, confirming that it enhanced antioxidant activity in vivo. This is the first study to successfully characterize natural antioxidant biomaterials created from marine invertebrate adhesives, and the findings indicate the excellent prospects of these biomaterials for great applications in tissue regeneration and cosmeceuticals.
format	article
author	Lulu Wang Xiaokang Zhang Pingping Xu Jicheng Yan Yuzhong Zhang Hainan Su Chengjun Sun Qiang Lu Weizhi Liu
author_facet	Lulu Wang Xiaokang Zhang Pingping Xu Jicheng Yan Yuzhong Zhang Hainan Su Chengjun Sun Qiang Lu Weizhi Liu
author_sort	Lulu Wang
title	Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity
title_short	Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity
title_full	Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity
title_fullStr	Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity
title_full_unstemmed	Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity
title_sort	exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/7f965126b586488c86a9a1a8cc705775
work_keys_str_mv	AT luluwang explorationofseaanemoneinspiredhighperformancebiomaterialswithenhancedantioxidantactivity AT xiaokangzhang explorationofseaanemoneinspiredhighperformancebiomaterialswithenhancedantioxidantactivity AT pingpingxu explorationofseaanemoneinspiredhighperformancebiomaterialswithenhancedantioxidantactivity AT jichengyan explorationofseaanemoneinspiredhighperformancebiomaterialswithenhancedantioxidantactivity AT yuzhongzhang explorationofseaanemoneinspiredhighperformancebiomaterialswithenhancedantioxidantactivity AT hainansu explorationofseaanemoneinspiredhighperformancebiomaterialswithenhancedantioxidantactivity AT chengjunsun explorationofseaanemoneinspiredhighperformancebiomaterialswithenhancedantioxidantactivity AT qianglu explorationofseaanemoneinspiredhighperformancebiomaterialswithenhancedantioxidantactivity AT weizhiliu explorationofseaanemoneinspiredhighperformancebiomaterialswithenhancedantioxidantactivity
_version_	1718406813887496192

Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity

Ejemplares similares