Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment

Metallic nanoparticles, such as gold nanoparticles (AuNPs), have been extensively studied as drug delivery systems for various therapeutic applications. However, drug-loaded-AuNPs have been rarely explored in vivo for their effect on bacteria residing inside tissues. Ciprofloxacin (CIP) is a second-...

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Autores principales:	Afrah Nawaz, Syed Mohsin Ali, Nosheen Fatima Rana, Tahreem Tanweer, Amna Batool, Thomas J. Webster, Farid Menaa, Sundus Riaz, Zahra Rehman, Farhat Batool, Misha Fatima, Tuba Maryam, Iqra Shafique, Abida Saleem, Arfa Iqbal
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	drug delivery antibiotics antimicrobial resistance gold nanoparticles ciprofloxacin <i>Enterococcus faecalis</i> Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/7f99b42ed573444ca15045685459abb1
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spelling	oai:doaj.org-article:7f99b42ed573444ca15045685459abb12021-11-25T18:32:51ZCiprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment10.3390/nano111131522079-4991https://doaj.org/article/7f99b42ed573444ca15045685459abb12021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/3152https://doaj.org/toc/2079-4991Metallic nanoparticles, such as gold nanoparticles (AuNPs), have been extensively studied as drug delivery systems for various therapeutic applications. However, drug-loaded-AuNPs have been rarely explored in vivo for their effect on bacteria residing inside tissues. Ciprofloxacin (CIP) is a second-generation fluoroquinolone with a broad-spectrum of antibiotic properties devoid of developing bacteria resistance. This research is focused on the synthesis and physical characterization of Ciprofloxacin-loaded gold nanoparticles (CIP-AuNPs) and their effect on the colonization of <i>Enterococcus faecalis</i> in the liver and kidneys of mice. The successfully prepared CIP-AuNPs were stable and exerted enhanced in vitro antibacterial activity against <i>E. faecalis</i> compared with free CIP. The optimized CIP-AuNPs were administered (500 µg/Kg) once a day via tail vein to infected mice for eight days and were found to be effective in eradicating <i>E. faecalis</i> from the host tissues. Moreover, unlike CIP, CIP-AuNPs were non-hemolytic. In summary, this study demonstrated that CIP-AuNPs are promising and biocompatible alternative therapeutics for <i>E.-faecalis</i>-induced infections resistant to conventional drugs (e.g., beta-lactams and vancomycin) and should be further investigated.Afrah NawazSyed Mohsin AliNosheen Fatima RanaTahreem TanweerAmna BatoolThomas J. WebsterFarid MenaaSundus RiazZahra RehmanFarhat BatoolMisha FatimaTuba MaryamIqra ShafiqueAbida SaleemArfa IqbalMDPI AGarticledrug deliveryantibioticsantimicrobial resistancegold nanoparticlesciprofloxacin<i>Enterococcus faecalis</i>ChemistryQD1-999ENNanomaterials, Vol 11, Iss 3152, p 3152 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	drug delivery antibiotics antimicrobial resistance gold nanoparticles ciprofloxacin <i>Enterococcus faecalis</i> Chemistry QD1-999
spellingShingle	drug delivery antibiotics antimicrobial resistance gold nanoparticles ciprofloxacin <i>Enterococcus faecalis</i> Chemistry QD1-999 Afrah Nawaz Syed Mohsin Ali Nosheen Fatima Rana Tahreem Tanweer Amna Batool Thomas J. Webster Farid Menaa Sundus Riaz Zahra Rehman Farhat Batool Misha Fatima Tuba Maryam Iqra Shafique Abida Saleem Arfa Iqbal Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment
description	Metallic nanoparticles, such as gold nanoparticles (AuNPs), have been extensively studied as drug delivery systems for various therapeutic applications. However, drug-loaded-AuNPs have been rarely explored in vivo for their effect on bacteria residing inside tissues. Ciprofloxacin (CIP) is a second-generation fluoroquinolone with a broad-spectrum of antibiotic properties devoid of developing bacteria resistance. This research is focused on the synthesis and physical characterization of Ciprofloxacin-loaded gold nanoparticles (CIP-AuNPs) and their effect on the colonization of <i>Enterococcus faecalis</i> in the liver and kidneys of mice. The successfully prepared CIP-AuNPs were stable and exerted enhanced in vitro antibacterial activity against <i>E. faecalis</i> compared with free CIP. The optimized CIP-AuNPs were administered (500 µg/Kg) once a day via tail vein to infected mice for eight days and were found to be effective in eradicating <i>E. faecalis</i> from the host tissues. Moreover, unlike CIP, CIP-AuNPs were non-hemolytic. In summary, this study demonstrated that CIP-AuNPs are promising and biocompatible alternative therapeutics for <i>E.-faecalis</i>-induced infections resistant to conventional drugs (e.g., beta-lactams and vancomycin) and should be further investigated.
format	article
author	Afrah Nawaz Syed Mohsin Ali Nosheen Fatima Rana Tahreem Tanweer Amna Batool Thomas J. Webster Farid Menaa Sundus Riaz Zahra Rehman Farhat Batool Misha Fatima Tuba Maryam Iqra Shafique Abida Saleem Arfa Iqbal
author_facet	Afrah Nawaz Syed Mohsin Ali Nosheen Fatima Rana Tahreem Tanweer Amna Batool Thomas J. Webster Farid Menaa Sundus Riaz Zahra Rehman Farhat Batool Misha Fatima Tuba Maryam Iqra Shafique Abida Saleem Arfa Iqbal
author_sort	Afrah Nawaz
title	Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment
title_short	Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment
title_full	Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment
title_fullStr	Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment
title_full_unstemmed	Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment
title_sort	ciprofloxacin-loaded gold nanoparticles against antimicrobial resistance: an in vivo assessment
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7f99b42ed573444ca15045685459abb1
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Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment

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