Lymphocytes as liver damage mirror of HCV related adipogenesis deregulation.

Hepatitis C virus infection leads to a wide spectrum of liver diseases ranging from mild chronic hepatitis to end-stage cirrhosis and hepatocellular carcinoma. An intriguing aspect of the HCV infection is its close connection with lipid metabolism playing an important role in the HCV life cycle and...

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Autores principales:	Antonella Minutolo, Beatrice Conti, Sandro Grelli, Carmela Viscomi, Giancarlo Labbadia, Clara Balsano
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7f9aba33d03a417a85dfa4cd25bf7c24
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spelling	oai:doaj.org-article:7f9aba33d03a417a85dfa4cd25bf7c242021-11-18T08:26:48ZLymphocytes as liver damage mirror of HCV related adipogenesis deregulation.1932-620310.1371/journal.pone.0092343https://doaj.org/article/7f9aba33d03a417a85dfa4cd25bf7c242014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24658135/?tool=EBIhttps://doaj.org/toc/1932-6203Hepatitis C virus infection leads to a wide spectrum of liver diseases ranging from mild chronic hepatitis to end-stage cirrhosis and hepatocellular carcinoma. An intriguing aspect of the HCV infection is its close connection with lipid metabolism playing an important role in the HCV life cycle and in its pathogenesis. HCV is known to be a hepatotropic virus; however, it can also infect peripheral blood mononuclear cells (PBMCs). The goal of the current investigation is to compare the adipogenesis profile of liver tissues to lymphocytes of HCV infected patients, in order to understand if PBMCs may reflect the alterations of intracellular pathways occurring during HCV-related liver steatosis. Using the Human Adipogenesis PCR Array, gene expression was analyzed in liver samples and PBMCs of chronic HCV+, HBV+ and Healthy Donors (HDs) patients. We observed a similar modulation of lipid metabolism in HCV+ and HBV+liver tissues and lymphoid, cells suggesting that PBMCs reflect the liver adipogenesis deregulation related to infection, even if the two viruses have a different impact in the regulation of the adipogenesis mechanisms. In particular, some genes involved in lipid metabolism and inflammation, as well as in cell transformation, were up-regulated, in a similar way, in both HCV models analyzed. Interestingly, these genes were positively correlated to virological and hepatic functional parameters of HCV+ patients. On the contrary, HBV+ patients displayed a completely different profile. PBMCs of HCV+ patients seem to be useful model to study how HCV-related lipid metabolism deregulation occurs in liver. The obtained data suggest some molecules as new possible biomarkers of HCV-related liver damage progression.Antonella MinutoloBeatrice ContiSandro GrelliCarmela ViscomiGiancarlo LabbadiaClara BalsanoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92343 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antonella Minutolo Beatrice Conti Sandro Grelli Carmela Viscomi Giancarlo Labbadia Clara Balsano Lymphocytes as liver damage mirror of HCV related adipogenesis deregulation.
description	Hepatitis C virus infection leads to a wide spectrum of liver diseases ranging from mild chronic hepatitis to end-stage cirrhosis and hepatocellular carcinoma. An intriguing aspect of the HCV infection is its close connection with lipid metabolism playing an important role in the HCV life cycle and in its pathogenesis. HCV is known to be a hepatotropic virus; however, it can also infect peripheral blood mononuclear cells (PBMCs). The goal of the current investigation is to compare the adipogenesis profile of liver tissues to lymphocytes of HCV infected patients, in order to understand if PBMCs may reflect the alterations of intracellular pathways occurring during HCV-related liver steatosis. Using the Human Adipogenesis PCR Array, gene expression was analyzed in liver samples and PBMCs of chronic HCV+, HBV+ and Healthy Donors (HDs) patients. We observed a similar modulation of lipid metabolism in HCV+ and HBV+liver tissues and lymphoid, cells suggesting that PBMCs reflect the liver adipogenesis deregulation related to infection, even if the two viruses have a different impact in the regulation of the adipogenesis mechanisms. In particular, some genes involved in lipid metabolism and inflammation, as well as in cell transformation, were up-regulated, in a similar way, in both HCV models analyzed. Interestingly, these genes were positively correlated to virological and hepatic functional parameters of HCV+ patients. On the contrary, HBV+ patients displayed a completely different profile. PBMCs of HCV+ patients seem to be useful model to study how HCV-related lipid metabolism deregulation occurs in liver. The obtained data suggest some molecules as new possible biomarkers of HCV-related liver damage progression.
format	article
author	Antonella Minutolo Beatrice Conti Sandro Grelli Carmela Viscomi Giancarlo Labbadia Clara Balsano
author_facet	Antonella Minutolo Beatrice Conti Sandro Grelli Carmela Viscomi Giancarlo Labbadia Clara Balsano
author_sort	Antonella Minutolo
title	Lymphocytes as liver damage mirror of HCV related adipogenesis deregulation.
title_short	Lymphocytes as liver damage mirror of HCV related adipogenesis deregulation.
title_full	Lymphocytes as liver damage mirror of HCV related adipogenesis deregulation.
title_fullStr	Lymphocytes as liver damage mirror of HCV related adipogenesis deregulation.
title_full_unstemmed	Lymphocytes as liver damage mirror of HCV related adipogenesis deregulation.
title_sort	lymphocytes as liver damage mirror of hcv related adipogenesis deregulation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7f9aba33d03a417a85dfa4cd25bf7c24
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Lymphocytes as liver damage mirror of HCV related adipogenesis deregulation.

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