Global Thrombosis Test: Occlusion is attributable to shear-induced platelet thrombus formation.

Herein, we set out a rebuttal to the publication by Claveria and co-workers published in TH Open this month entitled “Global Thrombosis Test: Occlusion by Coagulation or SIPA?” We strongly believe that the conclusions of their paper, suggesting that occlusion (OT) in the Global Thrombosis Test (GTT)...

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Autores principales:	Diana Adrienne Gorog, J. Yamamoto
Formato:	article
Lenguaje:	EN
Publicado:	Georg Thieme Verlag KG 2021
Materias:	Diseases of the circulatory (Cardiovascular) system RC666-701
Acceso en línea:	https://doaj.org/article/7fa0a67f685049e9845f413a6e4bc949
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spelling	oai:doaj.org-article:7fa0a67f685049e9845f413a6e4bc9492021-11-24T00:01:54ZGlobal Thrombosis Test: Occlusion is attributable to shear-induced platelet thrombus formation.2512-946510.1055/a-1704-1022https://doaj.org/article/7fa0a67f685049e9845f413a6e4bc9492021-11-01T00:00:00Zhttp://www.thieme-connect.de/DOI/DOI?10.1055/a-1704-1022https://doaj.org/toc/2512-9465Herein, we set out a rebuttal to the publication by Claveria and co-workers published in TH Open this month entitled “Global Thrombosis Test: Occlusion by Coagulation or SIPA?” We strongly believe that the conclusions of their paper, suggesting that occlusion (OT) in the Global Thrombosis Test (GTT) is due to coagulation, rather than shear-induced platelet thrombus formation, is incorrect and the evidence and arguments they present are fundamentally flawed, with major errors both in the experimental approach and in the interpretations of the results. The evidence which they demonstrate, shows that occlusion in the GTT is, in fact, caused by high shear induced platelet thrombus formations. We set out herein the evidence for that, based on histology of the thrombus from the GTT in earlier work using electron microscopy showing large platelet aggregates, the very brief timescale of OT in the GTT compared to coagulation time and the sensitivity of the OT in the GTT to the effects of heparin, t-PA and P2Y12 inhibitors. In addition, we revisit the known pathomechanism of high shear-mediated platelet aggregation to underpin our rationale and show that the modifications to the instrument proposed by Claveria and co-authors would render the technique unphysiological. We highlight several methodological concerns and apparent misinterpreted of the data obtained. We present evidence predominantly from the authors’ own data, together with our earlier published data and evidence from the literature, showing that occlusion in the GTT occurs do to shear-induced platelet aggregation.Diana Adrienne GorogJ. YamamotoGeorg Thieme Verlag KGarticleDiseases of the circulatory (Cardiovascular) systemRC666-701ENTH Open (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diseases of the circulatory (Cardiovascular) system RC666-701
spellingShingle	Diseases of the circulatory (Cardiovascular) system RC666-701 Diana Adrienne Gorog J. Yamamoto Global Thrombosis Test: Occlusion is attributable to shear-induced platelet thrombus formation.
description	Herein, we set out a rebuttal to the publication by Claveria and co-workers published in TH Open this month entitled “Global Thrombosis Test: Occlusion by Coagulation or SIPA?” We strongly believe that the conclusions of their paper, suggesting that occlusion (OT) in the Global Thrombosis Test (GTT) is due to coagulation, rather than shear-induced platelet thrombus formation, is incorrect and the evidence and arguments they present are fundamentally flawed, with major errors both in the experimental approach and in the interpretations of the results. The evidence which they demonstrate, shows that occlusion in the GTT is, in fact, caused by high shear induced platelet thrombus formations. We set out herein the evidence for that, based on histology of the thrombus from the GTT in earlier work using electron microscopy showing large platelet aggregates, the very brief timescale of OT in the GTT compared to coagulation time and the sensitivity of the OT in the GTT to the effects of heparin, t-PA and P2Y12 inhibitors. In addition, we revisit the known pathomechanism of high shear-mediated platelet aggregation to underpin our rationale and show that the modifications to the instrument proposed by Claveria and co-authors would render the technique unphysiological. We highlight several methodological concerns and apparent misinterpreted of the data obtained. We present evidence predominantly from the authors’ own data, together with our earlier published data and evidence from the literature, showing that occlusion in the GTT occurs do to shear-induced platelet aggregation.
format	article
author	Diana Adrienne Gorog J. Yamamoto
author_facet	Diana Adrienne Gorog J. Yamamoto
author_sort	Diana Adrienne Gorog
title	Global Thrombosis Test: Occlusion is attributable to shear-induced platelet thrombus formation.
title_short	Global Thrombosis Test: Occlusion is attributable to shear-induced platelet thrombus formation.
title_full	Global Thrombosis Test: Occlusion is attributable to shear-induced platelet thrombus formation.
title_fullStr	Global Thrombosis Test: Occlusion is attributable to shear-induced platelet thrombus formation.
title_full_unstemmed	Global Thrombosis Test: Occlusion is attributable to shear-induced platelet thrombus formation.
title_sort	global thrombosis test: occlusion is attributable to shear-induced platelet thrombus formation.
publisher	Georg Thieme Verlag KG
publishDate	2021
url	https://doaj.org/article/7fa0a67f685049e9845f413a6e4bc949
work_keys_str_mv	AT dianaadriennegorog globalthrombosistestocclusionisattributabletoshearinducedplateletthrombusformation AT jyamamoto globalthrombosistestocclusionisattributabletoshearinducedplateletthrombusformation
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Global Thrombosis Test: Occlusion is attributable to shear-induced platelet thrombus formation.

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