ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
CRISPR-Cas9 is a powerful genome engineering tool but gene knock-in is limited by fragment size and efficiency of recombination. Here the authors used a modified strategy employing single-strand oligonucleotides to efficiently knock-in large DNA fragments and humanise native rat loci.
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| Auteurs principaux: | , , , , , |
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| Format: | article |
| Langue: | EN |
| Publié: |
Nature Portfolio
2016
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| Sujets: | |
| Accès en ligne: | https://doaj.org/article/7fad07dee71e42a18e77cf83d887e8da |
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| Résumé: | CRISPR-Cas9 is a powerful genome engineering tool but gene knock-in is limited by fragment size and efficiency of recombination. Here the authors used a modified strategy employing single-strand oligonucleotides to efficiently knock-in large DNA fragments and humanise native rat loci. |
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