ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

CRISPR-Cas9 is a powerful genome engineering tool but gene knock-in is limited by fragment size and efficiency of recombination. Here the authors used a modified strategy employing single-strand oligonucleotides to efficiently knock-in large DNA fragments and humanise native rat loci.

Enregistré dans:

Détails bibliographiques
Auteurs principaux:	Kazuto Yoshimi, Yayoi Kunihiro, Takehito Kaneko, Hitoshi Nagahora, Birger Voigt, Tomoji Mashimo
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2016
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/7fad07dee71e42a18e77cf83d887e8da
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!

id	oai:doaj.org-article:7fad07dee71e42a18e77cf83d887e8da
record_format	dspace
spelling	oai:doaj.org-article:7fad07dee71e42a18e77cf83d887e8da2021-12-02T17:32:53ZssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes10.1038/ncomms104312041-1723https://doaj.org/article/7fad07dee71e42a18e77cf83d887e8da2016-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms10431https://doaj.org/toc/2041-1723CRISPR-Cas9 is a powerful genome engineering tool but gene knock-in is limited by fragment size and efficiency of recombination. Here the authors used a modified strategy employing single-strand oligonucleotides to efficiently knock-in large DNA fragments and humanise native rat loci.Kazuto YoshimiYayoi KunihiroTakehito KanekoHitoshi NagahoraBirger VoigtTomoji MashimoNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Kazuto Yoshimi Yayoi Kunihiro Takehito Kaneko Hitoshi Nagahora Birger Voigt Tomoji Mashimo ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
description	CRISPR-Cas9 is a powerful genome engineering tool but gene knock-in is limited by fragment size and efficiency of recombination. Here the authors used a modified strategy employing single-strand oligonucleotides to efficiently knock-in large DNA fragments and humanise native rat loci.
format	article
author	Kazuto Yoshimi Yayoi Kunihiro Takehito Kaneko Hitoshi Nagahora Birger Voigt Tomoji Mashimo
author_facet	Kazuto Yoshimi Yayoi Kunihiro Takehito Kaneko Hitoshi Nagahora Birger Voigt Tomoji Mashimo
author_sort	Kazuto Yoshimi
title	ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_short	ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_full	ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_fullStr	ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_full_unstemmed	ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_sort	ssodn-mediated knock-in with crispr-cas for large genomic regions in zygotes
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/7fad07dee71e42a18e77cf83d887e8da
work_keys_str_mv	AT kazutoyoshimi ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes AT yayoikunihiro ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes AT takehitokaneko ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes AT hitoshinagahora ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes AT birgervoigt ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes AT tomojimashimo ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes
_version_	1718380131259514880

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

Documents similaires