ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

CRISPR-Cas9 is a powerful genome engineering tool but gene knock-in is limited by fragment size and efficiency of recombination. Here the authors used a modified strategy employing single-strand oligonucleotides to efficiently knock-in large DNA fragments and humanise native rat loci.

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Autores principales: Kazuto Yoshimi, Yayoi Kunihiro, Takehito Kaneko, Hitoshi Nagahora, Birger Voigt, Tomoji Mashimo
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/7fad07dee71e42a18e77cf83d887e8da
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spelling oai:doaj.org-article:7fad07dee71e42a18e77cf83d887e8da2021-12-02T17:32:53ZssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes10.1038/ncomms104312041-1723https://doaj.org/article/7fad07dee71e42a18e77cf83d887e8da2016-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms10431https://doaj.org/toc/2041-1723CRISPR-Cas9 is a powerful genome engineering tool but gene knock-in is limited by fragment size and efficiency of recombination. Here the authors used a modified strategy employing single-strand oligonucleotides to efficiently knock-in large DNA fragments and humanise native rat loci.Kazuto YoshimiYayoi KunihiroTakehito KanekoHitoshi NagahoraBirger VoigtTomoji MashimoNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Kazuto Yoshimi
Yayoi Kunihiro
Takehito Kaneko
Hitoshi Nagahora
Birger Voigt
Tomoji Mashimo
ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
description CRISPR-Cas9 is a powerful genome engineering tool but gene knock-in is limited by fragment size and efficiency of recombination. Here the authors used a modified strategy employing single-strand oligonucleotides to efficiently knock-in large DNA fragments and humanise native rat loci.
format article
author Kazuto Yoshimi
Yayoi Kunihiro
Takehito Kaneko
Hitoshi Nagahora
Birger Voigt
Tomoji Mashimo
author_facet Kazuto Yoshimi
Yayoi Kunihiro
Takehito Kaneko
Hitoshi Nagahora
Birger Voigt
Tomoji Mashimo
author_sort Kazuto Yoshimi
title ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_short ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_full ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_fullStr ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_full_unstemmed ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
title_sort ssodn-mediated knock-in with crispr-cas for large genomic regions in zygotes
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/7fad07dee71e42a18e77cf83d887e8da
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AT yayoikunihiro ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes
AT takehitokaneko ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes
AT hitoshinagahora ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes
AT birgervoigt ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes
AT tomojimashimo ssodnmediatedknockinwithcrisprcasforlargegenomicregionsinzygotes
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