Polycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.

Regulation of viral transcription by chromatin structure has emerged as a fundamental determinant in the establishment of lytic and latent herpesvirus infections. The Polycomb group (PcG) of epigenetic repressors promotes heterochromatin formation by trimethylating histone H3 on lysine-27 (H3K27me3)...

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Autores principales:	Christopher G Abraham, Caroline A Kulesza
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fadff1e23ec496ba785149bef9312b8
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spelling	oai:doaj.org-article:7fadff1e23ec496ba785149bef9312b82021-11-18T07:29:59ZPolycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.1932-620310.1371/journal.pone.0029410https://doaj.org/article/7fadff1e23ec496ba785149bef9312b82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22279536/?tool=EBIhttps://doaj.org/toc/1932-6203Regulation of viral transcription by chromatin structure has emerged as a fundamental determinant in the establishment of lytic and latent herpesvirus infections. The Polycomb group (PcG) of epigenetic repressors promotes heterochromatin formation by trimethylating histone H3 on lysine-27 (H3K27me3) and regulates development, stem cell renewal and differentiation and the cell cycle. These cellular processes are tightly coupled to the molecular switch between lytic and latent herpesvirus infections. Using chromatin immunoprecipitation analysis, we observed enrichment of H3K27me3 at the major immediate-early (MIE) locus of murine cytomegalovirus (MCMV) very early following infection of permissive fibroblasts. As lytic replication progressed, we observed a loss of H3K27me3 enrichment concomitant with the appearance of H3K4me3. However, late during infection, as viral replication centers are established, we observed a significant increase in PcG protein association with chromatin. Additionally, in co-immunofluorescence assays using confocal microscopy, we detected strong enrichments for PcG protein within the viral replication compartment, suggesting an association between viral DNA synthesis machinery and PcG proteins. Together, our results suggest a novel, dynamic interaction between PcG epigenetic repressors and MCMV genomes.Christopher G AbrahamCaroline A KuleszaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29410 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christopher G Abraham Caroline A Kulesza Polycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.
description	Regulation of viral transcription by chromatin structure has emerged as a fundamental determinant in the establishment of lytic and latent herpesvirus infections. The Polycomb group (PcG) of epigenetic repressors promotes heterochromatin formation by trimethylating histone H3 on lysine-27 (H3K27me3) and regulates development, stem cell renewal and differentiation and the cell cycle. These cellular processes are tightly coupled to the molecular switch between lytic and latent herpesvirus infections. Using chromatin immunoprecipitation analysis, we observed enrichment of H3K27me3 at the major immediate-early (MIE) locus of murine cytomegalovirus (MCMV) very early following infection of permissive fibroblasts. As lytic replication progressed, we observed a loss of H3K27me3 enrichment concomitant with the appearance of H3K4me3. However, late during infection, as viral replication centers are established, we observed a significant increase in PcG protein association with chromatin. Additionally, in co-immunofluorescence assays using confocal microscopy, we detected strong enrichments for PcG protein within the viral replication compartment, suggesting an association between viral DNA synthesis machinery and PcG proteins. Together, our results suggest a novel, dynamic interaction between PcG epigenetic repressors and MCMV genomes.
format	article
author	Christopher G Abraham Caroline A Kulesza
author_facet	Christopher G Abraham Caroline A Kulesza
author_sort	Christopher G Abraham
title	Polycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.
title_short	Polycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.
title_full	Polycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.
title_fullStr	Polycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.
title_full_unstemmed	Polycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.
title_sort	polycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7fadff1e23ec496ba785149bef9312b8
work_keys_str_mv	AT christophergabraham polycombrepressivecomplex2targetsmurinecytomegaloviruschromatinformodificationandassociateswithviralreplicationcenters AT carolineakulesza polycombrepressivecomplex2targetsmurinecytomegaloviruschromatinformodificationandassociateswithviralreplicationcenters
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Polycomb repressive complex 2 targets murine cytomegalovirus chromatin for modification and associates with viral replication centers.

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