Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos

Abstract Imaging of living cells based on traditional fluorescence and confocal laser scanning microscopy has delivered an enormous amount of information critical for understanding biological processes in single cells. However, the requirement for a high numerical aperture and fluorescent markers st...

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Autores principales:	Karol Karnowski, Anna Ajduk, Bartosz Wieloch, Szymon Tamborski, Krzysztof Krawiec, Maciej Wojtkowski, Maciej Szkulmowski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fb18e6618ac492b86446429f92a5039
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spelling	oai:doaj.org-article:7fb18e6618ac492b86446429f92a50392021-12-02T11:52:40ZOptical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos10.1038/s41598-017-04220-82045-2322https://doaj.org/article/7fb18e6618ac492b86446429f92a50392017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04220-8https://doaj.org/toc/2045-2322Abstract Imaging of living cells based on traditional fluorescence and confocal laser scanning microscopy has delivered an enormous amount of information critical for understanding biological processes in single cells. However, the requirement for a high numerical aperture and fluorescent markers still limits researchers’ ability to visualize the cellular architecture without causing short- and long-term photodamage. Optical coherence microscopy (OCM) is a promising alternative that circumvents the technical limitations of fluorescence imaging techniques and provides unique access to fundamental aspects of early embryonic development, without the requirement for sample pre-processing or labeling. In the present paper, we utilized the internal motion of cytoplasm, as well as custom scanning and signal processing protocols, to effectively reduce the speckle noise typical for standard OCM and enable high-resolution intracellular time-lapse imaging. To test our imaging system we used mouse and pig oocytes and embryos and visualized them through fertilization and the first embryonic division, as well as at selected stages of oogenesis and preimplantation development. Because all morphological and morphokinetic properties recorded by OCM are believed to be biomarkers of oocyte/embryo quality, OCM may represent a new chapter in imaging-based preimplantation embryo diagnostics.Karol KarnowskiAnna AjdukBartosz WielochSzymon TamborskiKrzysztof KrawiecMaciej WojtkowskiMaciej SzkulmowskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karol Karnowski Anna Ajduk Bartosz Wieloch Szymon Tamborski Krzysztof Krawiec Maciej Wojtkowski Maciej Szkulmowski Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos
description	Abstract Imaging of living cells based on traditional fluorescence and confocal laser scanning microscopy has delivered an enormous amount of information critical for understanding biological processes in single cells. However, the requirement for a high numerical aperture and fluorescent markers still limits researchers’ ability to visualize the cellular architecture without causing short- and long-term photodamage. Optical coherence microscopy (OCM) is a promising alternative that circumvents the technical limitations of fluorescence imaging techniques and provides unique access to fundamental aspects of early embryonic development, without the requirement for sample pre-processing or labeling. In the present paper, we utilized the internal motion of cytoplasm, as well as custom scanning and signal processing protocols, to effectively reduce the speckle noise typical for standard OCM and enable high-resolution intracellular time-lapse imaging. To test our imaging system we used mouse and pig oocytes and embryos and visualized them through fertilization and the first embryonic division, as well as at selected stages of oogenesis and preimplantation development. Because all morphological and morphokinetic properties recorded by OCM are believed to be biomarkers of oocyte/embryo quality, OCM may represent a new chapter in imaging-based preimplantation embryo diagnostics.
format	article
author	Karol Karnowski Anna Ajduk Bartosz Wieloch Szymon Tamborski Krzysztof Krawiec Maciej Wojtkowski Maciej Szkulmowski
author_facet	Karol Karnowski Anna Ajduk Bartosz Wieloch Szymon Tamborski Krzysztof Krawiec Maciej Wojtkowski Maciej Szkulmowski
author_sort	Karol Karnowski
title	Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos
title_short	Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos
title_full	Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos
title_fullStr	Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos
title_full_unstemmed	Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos
title_sort	optical coherence microscopy as a novel, non-invasive method for the 4d live imaging of early mammalian embryos
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7fb18e6618ac492b86446429f92a5039
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Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos

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