Intensity of free radical processes in rats’ blood while deep hypothermia and self-warming

Hypothermic conditions of homoisothermic organisms are characterized by the activation of free-radical processes in tissues. The intensity of these processes occurring at hypothermia is less well understood. The essential increase in heart rate, breathing, blood flow velocity, and metabolic processe...

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Autores principales:	N. K. Klichkhanov, Z. G. Ismailova, M. D. Astaeva
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	rats blood hypothermia self-warming plasma proteins malondialdehyde protein carbonyl groups antioxidant enzymes Science Q
Acceso en línea:	https://doaj.org/article/7fb22c9e779642c29781a8d282b61bbc
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spelling	oai:doaj.org-article:7fb22c9e779642c29781a8d282b61bbc2021-11-23T06:14:36ZIntensity of free radical processes in rats’ blood while deep hypothermia and self-warming2541-94202587-959610.12737/23402https://doaj.org/article/7fb22c9e779642c29781a8d282b61bbc2016-09-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/288https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Hypothermic conditions of homoisothermic organisms are characterized by the activation of free-radical processes in tissues. The intensity of these processes occurring at hypothermia is less well understood. The essential increase in heart rate, breathing, blood flow velocity, and metabolic processes during warming must stimulate the generation of reactive oxygen species and oxidative modification of biomolecules. We study the levels of peroxidation markers of lipids (by malondialdehyde) and proteins (by carbonyl groups) in blood plasma and erythrocytes as well as the activity of erythrocyte antioxidant enzymes of rats after deep hypothermia (the temperature in the rectum was 20 °C) and self-warming dynamics. A maximum warming rate (0.0160C/min) was revealed over the body temperature range of 22-33 °С, below and above these temperatures a warming rate was essentially lower. The warming of rats resulted in a total protein content reduction which negatively correlates (r = -0.967; р < 0.05) with a middle molecular peptide level. The deep hypothermia decreased the intensity of oxidative modification of lipid and proteins in blood plasma and red blood cell membranes, and the activity of red blood cell superoxide dismutase (SOD). Maximum amount of products of oxidative modification of lipids and proteins in plasma and erythrocytes membranes caused by rats' self-warming was observed at body temperature of 30-35 °С. After a complete rats' warming the intensity of oxidative modification of lipids and proteins in plasma and erythrocyte membranes decreased. The activity of SOD and catalase of erythrocyte substantially increased when body temperature reached 35 °С. The obtained data indicate that during self-warming at the body temperature of 30-35 °С the oxidative stress appears in blood which requires the use of antioxidant defense.N. K. KlichkhanovZ. G. IsmailovaM. D. AstaevaScientific Сentre for Family Health and Human Reproduction Problemsarticleratsbloodhypothermiaself-warmingplasma proteinsmalondialdehydeprotein carbonyl groupsantioxidant enzymesScienceQRUActa Biomedica Scientifica, Vol 1, Iss 5, Pp 104-109 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	rats blood hypothermia self-warming plasma proteins malondialdehyde protein carbonyl groups antioxidant enzymes Science Q
spellingShingle	rats blood hypothermia self-warming plasma proteins malondialdehyde protein carbonyl groups antioxidant enzymes Science Q N. K. Klichkhanov Z. G. Ismailova M. D. Astaeva Intensity of free radical processes in rats’ blood while deep hypothermia and self-warming
description	Hypothermic conditions of homoisothermic organisms are characterized by the activation of free-radical processes in tissues. The intensity of these processes occurring at hypothermia is less well understood. The essential increase in heart rate, breathing, blood flow velocity, and metabolic processes during warming must stimulate the generation of reactive oxygen species and oxidative modification of biomolecules. We study the levels of peroxidation markers of lipids (by malondialdehyde) and proteins (by carbonyl groups) in blood plasma and erythrocytes as well as the activity of erythrocyte antioxidant enzymes of rats after deep hypothermia (the temperature in the rectum was 20 °C) and self-warming dynamics. A maximum warming rate (0.0160C/min) was revealed over the body temperature range of 22-33 °С, below and above these temperatures a warming rate was essentially lower. The warming of rats resulted in a total protein content reduction which negatively correlates (r = -0.967; р < 0.05) with a middle molecular peptide level. The deep hypothermia decreased the intensity of oxidative modification of lipid and proteins in blood plasma and red blood cell membranes, and the activity of red blood cell superoxide dismutase (SOD). Maximum amount of products of oxidative modification of lipids and proteins in plasma and erythrocytes membranes caused by rats' self-warming was observed at body temperature of 30-35 °С. After a complete rats' warming the intensity of oxidative modification of lipids and proteins in plasma and erythrocyte membranes decreased. The activity of SOD and catalase of erythrocyte substantially increased when body temperature reached 35 °С. The obtained data indicate that during self-warming at the body temperature of 30-35 °С the oxidative stress appears in blood which requires the use of antioxidant defense.
format	article
author	N. K. Klichkhanov Z. G. Ismailova M. D. Astaeva
author_facet	N. K. Klichkhanov Z. G. Ismailova M. D. Astaeva
author_sort	N. K. Klichkhanov
title	Intensity of free radical processes in rats’ blood while deep hypothermia and self-warming
title_short	Intensity of free radical processes in rats’ blood while deep hypothermia and self-warming
title_full	Intensity of free radical processes in rats’ blood while deep hypothermia and self-warming
title_fullStr	Intensity of free radical processes in rats’ blood while deep hypothermia and self-warming
title_full_unstemmed	Intensity of free radical processes in rats’ blood while deep hypothermia and self-warming
title_sort	intensity of free radical processes in rats’ blood while deep hypothermia and self-warming
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/7fb22c9e779642c29781a8d282b61bbc
work_keys_str_mv	AT nkklichkhanov intensityoffreeradicalprocessesinratsbloodwhiledeephypothermiaandselfwarming AT zgismailova intensityoffreeradicalprocessesinratsbloodwhiledeephypothermiaandselfwarming AT mdastaeva intensityoffreeradicalprocessesinratsbloodwhiledeephypothermiaandselfwarming
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Intensity of free radical processes in rats’ blood while deep hypothermia and self-warming

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