Structural analysis of Wss1 protein from saccharomyces cerevisiae

Abstract Wss1 is a DNA-protein crosslinks (DPCs) repair protein, which is responsible for degradation of the protein components in DPCs. In this investigation, crystal structure of the protease domain from saccharomyces cerevisiae Wss1 (ScWss1) was solved and was compared with the known crystal stru...

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Autores principales:	Xiaoyun Yang, Yanhua Li, Zengqiang Gao, Zongqiang Li, Jianhua Xu, Wenjia Wang, Yuhui Dong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fb2675efc6748e98d4e1ef53b356b3a
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spelling	oai:doaj.org-article:7fb2675efc6748e98d4e1ef53b356b3a2021-12-02T15:05:28ZStructural analysis of Wss1 protein from saccharomyces cerevisiae10.1038/s41598-017-08834-w2045-2322https://doaj.org/article/7fb2675efc6748e98d4e1ef53b356b3a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08834-whttps://doaj.org/toc/2045-2322Abstract Wss1 is a DNA-protein crosslinks (DPCs) repair protein, which is responsible for degradation of the protein components in DPCs. In this investigation, crystal structure of the protease domain from saccharomyces cerevisiae Wss1 (ScWss1) was solved and was compared with the known crystal structure of Schizosaccharomyces prombe Wss1 (SpWss1). It is found that the cleft near zinc ion to be the most conserved core region of Wss1 and that the electronic surface distributions vary greatly between the two homologs. Solution architecture of the full-length ScWss1 was further investigated by small-angle X-ray scattering (SAXS), which indicated the protein contains a flexible region inside. Finally, based on the structural information, a mechanism was proposed about how the enzyme is activated by DNA substrates.Xiaoyun YangYanhua LiZengqiang GaoZongqiang LiJianhua XuWenjia WangYuhui DongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoyun Yang Yanhua Li Zengqiang Gao Zongqiang Li Jianhua Xu Wenjia Wang Yuhui Dong Structural analysis of Wss1 protein from saccharomyces cerevisiae
description	Abstract Wss1 is a DNA-protein crosslinks (DPCs) repair protein, which is responsible for degradation of the protein components in DPCs. In this investigation, crystal structure of the protease domain from saccharomyces cerevisiae Wss1 (ScWss1) was solved and was compared with the known crystal structure of Schizosaccharomyces prombe Wss1 (SpWss1). It is found that the cleft near zinc ion to be the most conserved core region of Wss1 and that the electronic surface distributions vary greatly between the two homologs. Solution architecture of the full-length ScWss1 was further investigated by small-angle X-ray scattering (SAXS), which indicated the protein contains a flexible region inside. Finally, based on the structural information, a mechanism was proposed about how the enzyme is activated by DNA substrates.
format	article
author	Xiaoyun Yang Yanhua Li Zengqiang Gao Zongqiang Li Jianhua Xu Wenjia Wang Yuhui Dong
author_facet	Xiaoyun Yang Yanhua Li Zengqiang Gao Zongqiang Li Jianhua Xu Wenjia Wang Yuhui Dong
author_sort	Xiaoyun Yang
title	Structural analysis of Wss1 protein from saccharomyces cerevisiae
title_short	Structural analysis of Wss1 protein from saccharomyces cerevisiae
title_full	Structural analysis of Wss1 protein from saccharomyces cerevisiae
title_fullStr	Structural analysis of Wss1 protein from saccharomyces cerevisiae
title_full_unstemmed	Structural analysis of Wss1 protein from saccharomyces cerevisiae
title_sort	structural analysis of wss1 protein from saccharomyces cerevisiae
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7fb2675efc6748e98d4e1ef53b356b3a
work_keys_str_mv	AT xiaoyunyang structuralanalysisofwss1proteinfromsaccharomycescerevisiae AT yanhuali structuralanalysisofwss1proteinfromsaccharomycescerevisiae AT zengqianggao structuralanalysisofwss1proteinfromsaccharomycescerevisiae AT zongqiangli structuralanalysisofwss1proteinfromsaccharomycescerevisiae AT jianhuaxu structuralanalysisofwss1proteinfromsaccharomycescerevisiae AT wenjiawang structuralanalysisofwss1proteinfromsaccharomycescerevisiae AT yuhuidong structuralanalysisofwss1proteinfromsaccharomycescerevisiae
_version_	1718388841884155904

Structural analysis of Wss1 protein from saccharomyces cerevisiae

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