Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.

Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promisin...

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Autores principales:	Ana Clara Mignaqui, Vanesa Ruiz, Sylvie Perret, Gilles St-Laurent, Parminder Singh Chahal, Julia Transfiguracion, Ayelén Sammarruco, Victoria Gnazzo, Yves Durocher, Andrés Wigdorovitz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fb6776d7961411fb751d60edea38880
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spelling	oai:doaj.org-article:7fb6776d7961411fb751d60edea388802021-11-18T08:58:43ZTransient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.1932-620310.1371/journal.pone.0072800https://doaj.org/article/7fb6776d7961411fb751d60edea388802013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23977353/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.Ana Clara MignaquiVanesa RuizSylvie PerretGilles St-LaurentParminder Singh ChahalJulia TransfiguracionAyelén SammarrucoVictoria GnazzoYves DurocherAndrés WigdorovitzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e72800 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Clara Mignaqui Vanesa Ruiz Sylvie Perret Gilles St-Laurent Parminder Singh Chahal Julia Transfiguracion Ayelén Sammarruco Victoria Gnazzo Yves Durocher Andrés Wigdorovitz Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.
description	Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
format	article
author	Ana Clara Mignaqui Vanesa Ruiz Sylvie Perret Gilles St-Laurent Parminder Singh Chahal Julia Transfiguracion Ayelén Sammarruco Victoria Gnazzo Yves Durocher Andrés Wigdorovitz
author_facet	Ana Clara Mignaqui Vanesa Ruiz Sylvie Perret Gilles St-Laurent Parminder Singh Chahal Julia Transfiguracion Ayelén Sammarruco Victoria Gnazzo Yves Durocher Andrés Wigdorovitz
author_sort	Ana Clara Mignaqui
title	Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.
title_short	Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.
title_full	Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.
title_fullStr	Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.
title_full_unstemmed	Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.
title_sort	transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7fb6776d7961411fb751d60edea38880
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Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids.

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