Potential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance

Background. Cancer stem cells (CSCs) are responsible for tumorigenesis, chemoresistance, and metastasis. Chemoresistance is a major challenge in the management of lung cancer. Glutathione-sulphur-transferase-π (GST-π) plays an important role in the origin and development of various types of cancer b...

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Autores principales:	Wenjun Wang, Jianping Wei, Xiaoyun Tu, Xiaoqun Ye
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/7fb7ec320cf741d8a74fb02b742c3bb2
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spelling	oai:doaj.org-article:7fb7ec320cf741d8a74fb02b742c3bb22021-11-29T00:57:04ZPotential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance2314-614110.1155/2021/9142364https://doaj.org/article/7fb7ec320cf741d8a74fb02b742c3bb22021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9142364https://doaj.org/toc/2314-6141Background. Cancer stem cells (CSCs) are responsible for tumorigenesis, chemoresistance, and metastasis. Chemoresistance is a major challenge in the management of lung cancer. Glutathione-sulphur-transferase-π (GST-π) plays an important role in the origin and development of various types of cancer by regulating the cellular redox balance. Recent investigations have demonstrated that GST-π is associated with the chemoresistance of lung CSCs (LCSCs). However, the mechanism of GST-π in lung cancer, particularly in LCSCs, remains unclear. The present study is aimed at exploring the potential role of GST-π in stemness and cisplatin (DDP) resistance of LCSCs. Materials and methods. In the present study, lung cancer cell spheres were established using the A549 cell line, which according to our previous research, was confirmed to exhibit characteristics of stem cells. Next, GST-π protein expression, apoptosis percentage, and intracellular reactive oxygen species (ROS) concentration in A549 adherent cells and A549 cell spheres were analyzed by western blotting and flow cytometry, respectively. Finally, DDP resistance, ROS concentration, and GST-π expression in LCSCs were analyzed following the interference with GST-π using DL-buthionine-(S,R)-sulphoximine and N-acetylcysteine. Results. The results revealed that GST-π was highly expressed in A549 cell spheres compared with A549 adherent cells and was associated with a decreased intracellular ROS concentration (both P<0.05). Regulating GST-π protein expression could alter DDP resistance of LCSCs by influencing ROS. Conclusion. These results suggested that GST-π may be important for LCSC drug resistance by downregulating ROS levels. These findings may contribute to the development of new adjuvant therapeutic strategies for lung cancer.Wenjun WangJianping WeiXiaoyun TuXiaoqun YeHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Wenjun Wang Jianping Wei Xiaoyun Tu Xiaoqun Ye Potential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance
description	Background. Cancer stem cells (CSCs) are responsible for tumorigenesis, chemoresistance, and metastasis. Chemoresistance is a major challenge in the management of lung cancer. Glutathione-sulphur-transferase-π (GST-π) plays an important role in the origin and development of various types of cancer by regulating the cellular redox balance. Recent investigations have demonstrated that GST-π is associated with the chemoresistance of lung CSCs (LCSCs). However, the mechanism of GST-π in lung cancer, particularly in LCSCs, remains unclear. The present study is aimed at exploring the potential role of GST-π in stemness and cisplatin (DDP) resistance of LCSCs. Materials and methods. In the present study, lung cancer cell spheres were established using the A549 cell line, which according to our previous research, was confirmed to exhibit characteristics of stem cells. Next, GST-π protein expression, apoptosis percentage, and intracellular reactive oxygen species (ROS) concentration in A549 adherent cells and A549 cell spheres were analyzed by western blotting and flow cytometry, respectively. Finally, DDP resistance, ROS concentration, and GST-π expression in LCSCs were analyzed following the interference with GST-π using DL-buthionine-(S,R)-sulphoximine and N-acetylcysteine. Results. The results revealed that GST-π was highly expressed in A549 cell spheres compared with A549 adherent cells and was associated with a decreased intracellular ROS concentration (both P<0.05). Regulating GST-π protein expression could alter DDP resistance of LCSCs by influencing ROS. Conclusion. These results suggested that GST-π may be important for LCSC drug resistance by downregulating ROS levels. These findings may contribute to the development of new adjuvant therapeutic strategies for lung cancer.
format	article
author	Wenjun Wang Jianping Wei Xiaoyun Tu Xiaoqun Ye
author_facet	Wenjun Wang Jianping Wei Xiaoyun Tu Xiaoqun Ye
author_sort	Wenjun Wang
title	Potential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance
title_short	Potential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance
title_full	Potential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance
title_fullStr	Potential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance
title_full_unstemmed	Potential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance
title_sort	potential role of gst-π in lung cancer stem cell cisplatin resistance
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/7fb7ec320cf741d8a74fb02b742c3bb2
work_keys_str_mv	AT wenjunwang potentialroleofgstpinlungcancerstemcellcisplatinresistance AT jianpingwei potentialroleofgstpinlungcancerstemcellcisplatinresistance AT xiaoyuntu potentialroleofgstpinlungcancerstemcellcisplatinresistance AT xiaoqunye potentialroleofgstpinlungcancerstemcellcisplatinresistance
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Potential Role of GST-π in Lung Cancer Stem Cell Cisplatin Resistance

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