Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification.
Non-coding transcription can trigger histone post-translational modifications forming specialized chromatin. In fission yeast, heterochromatin formation requires RNAi and the histone H3K9 methyltransferase complex CLRC, composed of Clr4, Raf1, Raf2, Cul4, and Rik1. CLRC mediates H3K9 methylation and...
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2012
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oai:doaj.org-article:7fba01400f744702854789e4804d689e2021-11-18T06:18:59ZRaf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification.1553-73901553-740410.1371/journal.pgen.1002499https://doaj.org/article/7fba01400f744702854789e4804d689e2012-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22319459/pdf/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Non-coding transcription can trigger histone post-translational modifications forming specialized chromatin. In fission yeast, heterochromatin formation requires RNAi and the histone H3K9 methyltransferase complex CLRC, composed of Clr4, Raf1, Raf2, Cul4, and Rik1. CLRC mediates H3K9 methylation and siRNA production; it also displays E3-ubiquitin ligase activity in vitro. DCAFs act as substrate receptors for E3 ligases and may couple ubiquitination with histone methylation. Here, structural alignment and mutation of signature WDxR motifs in Raf1 indicate that it is a DCAF for CLRC. We demonstrate that Raf1 promotes H3K9 methylation and siRNA amplification via two distinct, separable functions. The association of the DCAF Raf1 with Cul4-Rik1 is critical for H3K9 methylation, but dispensable for processing of centromeric transcripts into siRNAs. Thus the association of a DCAF, Raf1, with its adaptor, Rik1, is required for histone methylation and to allow RNAi to signal to chromatin.Alessia BuscainoSharon A WhiteDouglas R HoustonErwan LejeuneFemke SimmerFlavia de Lima AlvesPiyush T DiyoraTakeshi UranoElizabeth H BayneJuri RappsilberRobin C AllshirePublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 8, Iss 2, p e1002499 (2012) |
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DOAJ |
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DOAJ |
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Genetics QH426-470 |
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Genetics QH426-470 Alessia Buscaino Sharon A White Douglas R Houston Erwan Lejeune Femke Simmer Flavia de Lima Alves Piyush T Diyora Takeshi Urano Elizabeth H Bayne Juri Rappsilber Robin C Allshire Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification. |
| description |
Non-coding transcription can trigger histone post-translational modifications forming specialized chromatin. In fission yeast, heterochromatin formation requires RNAi and the histone H3K9 methyltransferase complex CLRC, composed of Clr4, Raf1, Raf2, Cul4, and Rik1. CLRC mediates H3K9 methylation and siRNA production; it also displays E3-ubiquitin ligase activity in vitro. DCAFs act as substrate receptors for E3 ligases and may couple ubiquitination with histone methylation. Here, structural alignment and mutation of signature WDxR motifs in Raf1 indicate that it is a DCAF for CLRC. We demonstrate that Raf1 promotes H3K9 methylation and siRNA amplification via two distinct, separable functions. The association of the DCAF Raf1 with Cul4-Rik1 is critical for H3K9 methylation, but dispensable for processing of centromeric transcripts into siRNAs. Thus the association of a DCAF, Raf1, with its adaptor, Rik1, is required for histone methylation and to allow RNAi to signal to chromatin. |
| format |
article |
| author |
Alessia Buscaino Sharon A White Douglas R Houston Erwan Lejeune Femke Simmer Flavia de Lima Alves Piyush T Diyora Takeshi Urano Elizabeth H Bayne Juri Rappsilber Robin C Allshire |
| author_facet |
Alessia Buscaino Sharon A White Douglas R Houston Erwan Lejeune Femke Simmer Flavia de Lima Alves Piyush T Diyora Takeshi Urano Elizabeth H Bayne Juri Rappsilber Robin C Allshire |
| author_sort |
Alessia Buscaino |
| title |
Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification. |
| title_short |
Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification. |
| title_full |
Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification. |
| title_fullStr |
Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification. |
| title_full_unstemmed |
Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification. |
| title_sort |
raf1 is a dcaf for the rik1 ddb1-like protein and has separable roles in sirna generation and chromatin modification. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/7fba01400f744702854789e4804d689e |
| work_keys_str_mv |
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