AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia

Coordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF...

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Autores principales:	Shimin Wang, Longfeng Yao, Wenjuan Zhang, Zihang Cheng, Can Hu, Hang Liu, Yanling Yan, Anbing Shi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	C. elegans polarized sorting SMAP-1/SMAPs AP-1 clathrin intestinal epithelia Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7fbb4c8fe023457d8f9bccb21bca0160
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spelling	oai:doaj.org-article:7fbb4c8fe023457d8f9bccb21bca01602021-12-01T00:41:24ZAP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia2296-634X10.3389/fcell.2021.774401https://doaj.org/article/7fbb4c8fe023457d8f9bccb21bca01602021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.774401/fullhttps://doaj.org/toc/2296-634XCoordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF-1 appeared abnormally on the apical membrane. SMAP-1 colocalized with AP-1 on the TGN. The integrity of AP-1 is required for the subcellular presence of SMAP-1. Moreover, we found that the loss of SMAP-1 reduced clathrin-positive structures in the cytosol, suggesting that SMAP-1 has a regulatory role in clathrin assembly on the TGN. Functional experiments showed that overexpressing clathrin effectively alleviated exocytic defects due to the lack of SMAP-1, corroborating the role of SMAP-1 in promoting the assembly of clathrin on the TGN. Together, our results suggested that the AP-1 complex regulates the TGN localization of SMAP-1, promoting clathrin assembly to ensure polarized conventional secretion in C. elegans intestinal epithelia.Shimin WangLongfeng YaoWenjuan ZhangZihang ChengCan HuHang LiuYanling YanYanling YanAnbing ShiAnbing ShiFrontiers Media S.A.articleC. eleganspolarized sortingSMAP-1/SMAPsAP-1clathrinintestinal epitheliaBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	C. elegans polarized sorting SMAP-1/SMAPs AP-1 clathrin intestinal epithelia Biology (General) QH301-705.5
spellingShingle	C. elegans polarized sorting SMAP-1/SMAPs AP-1 clathrin intestinal epithelia Biology (General) QH301-705.5 Shimin Wang Longfeng Yao Wenjuan Zhang Zihang Cheng Can Hu Hang Liu Yanling Yan Yanling Yan Anbing Shi Anbing Shi AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
description	Coordinated AP-1 and clathrin coat assembly mediate secretory sorting on the trans-Golgi network (TGN) during conventional secretion. Here we found that SMAP-1/SMAPs deficiency caused the apical protein ERM-1 to accumulate on the basolateral side of the TGN. In contrast, the basolateral protein SLCF-1 appeared abnormally on the apical membrane. SMAP-1 colocalized with AP-1 on the TGN. The integrity of AP-1 is required for the subcellular presence of SMAP-1. Moreover, we found that the loss of SMAP-1 reduced clathrin-positive structures in the cytosol, suggesting that SMAP-1 has a regulatory role in clathrin assembly on the TGN. Functional experiments showed that overexpressing clathrin effectively alleviated exocytic defects due to the lack of SMAP-1, corroborating the role of SMAP-1 in promoting the assembly of clathrin on the TGN. Together, our results suggested that the AP-1 complex regulates the TGN localization of SMAP-1, promoting clathrin assembly to ensure polarized conventional secretion in C. elegans intestinal epithelia.
format	article
author	Shimin Wang Longfeng Yao Wenjuan Zhang Zihang Cheng Can Hu Hang Liu Yanling Yan Yanling Yan Anbing Shi Anbing Shi
author_facet	Shimin Wang Longfeng Yao Wenjuan Zhang Zihang Cheng Can Hu Hang Liu Yanling Yan Yanling Yan Anbing Shi Anbing Shi
author_sort	Shimin Wang
title	AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_short	AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_full	AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_fullStr	AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_full_unstemmed	AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia
title_sort	ap-1 recruits smap-1/smaps to the trans-golgi network to promote sorting in polarized epithelia
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/7fbb4c8fe023457d8f9bccb21bca0160
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AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia

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