The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model

Abstract The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). I...

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Autores principales:	Yu Okuma, Tomoaki Aoki, Santiago J. Miyara, Kei Hayashida, Mitsuaki Nishikimi, Ryosuke Takegawa, Tai Yin, Junhwan Kim, Lance B. Becker, Koichiro Shinozaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fc1625c4de34b6c93af84813e35c18c
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spelling	oai:doaj.org-article:7fc1625c4de34b6c93af84813e35c18c2021-12-02T15:23:00ZThe evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model10.1038/s41598-020-79780-32045-2322https://doaj.org/article/7fc1625c4de34b6c93af84813e35c18c2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79780-3https://doaj.org/toc/2045-2322Abstract The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). Immunohistochemistry and ELISA assays were used to evaluate the pituitary damage and endocrine function. Samples were collected at pre-CA, and 30 and 120 min after cardio pulmonary resuscitation. Triphenyltetrazolium chloride (TTC) staining demonstrated the expansion of the pituitary damage over time. There was phenotypic validity between the pars distalis and nervosa. Both CT-proAVP (pars nervosa hormone) and GH/IGF-1 (pars distalis hormone) decreased over time, and a different expression pattern corresponding to the damaged areas was noted (CT-proAVP, 30.2 ± 6.2, 31.5 ± 5.9, and 16.3 ± 7.6 pg/mg protein, p < 0.01; GH/IGF-1, 2.63 ± 0.61, 0.62 ± 0.36, and 2.01 ± 0.41 ng/mg protein, p < 0.01 respectively). Similarly, the expression pattern between these hormones in the end-organ systems showed phenotypic validity. Plasma CT-proAVP (r = 0.771, p = 0.025) and IGF-1 (r = −0.775, p = 0.024) demonstrated a strong correlation with TTC staining area. Our data suggested that CA induces pathological and functional damage to the pituitary gland.Yu OkumaTomoaki AokiSantiago J. MiyaraKei HayashidaMitsuaki NishikimiRyosuke TakegawaTai YinJunhwan KimLance B. BeckerKoichiro ShinozakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu Okuma Tomoaki Aoki Santiago J. Miyara Kei Hayashida Mitsuaki Nishikimi Ryosuke Takegawa Tai Yin Junhwan Kim Lance B. Becker Koichiro Shinozaki The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model
description	Abstract The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). Immunohistochemistry and ELISA assays were used to evaluate the pituitary damage and endocrine function. Samples were collected at pre-CA, and 30 and 120 min after cardio pulmonary resuscitation. Triphenyltetrazolium chloride (TTC) staining demonstrated the expansion of the pituitary damage over time. There was phenotypic validity between the pars distalis and nervosa. Both CT-proAVP (pars nervosa hormone) and GH/IGF-1 (pars distalis hormone) decreased over time, and a different expression pattern corresponding to the damaged areas was noted (CT-proAVP, 30.2 ± 6.2, 31.5 ± 5.9, and 16.3 ± 7.6 pg/mg protein, p < 0.01; GH/IGF-1, 2.63 ± 0.61, 0.62 ± 0.36, and 2.01 ± 0.41 ng/mg protein, p < 0.01 respectively). Similarly, the expression pattern between these hormones in the end-organ systems showed phenotypic validity. Plasma CT-proAVP (r = 0.771, p = 0.025) and IGF-1 (r = −0.775, p = 0.024) demonstrated a strong correlation with TTC staining area. Our data suggested that CA induces pathological and functional damage to the pituitary gland.
format	article
author	Yu Okuma Tomoaki Aoki Santiago J. Miyara Kei Hayashida Mitsuaki Nishikimi Ryosuke Takegawa Tai Yin Junhwan Kim Lance B. Becker Koichiro Shinozaki
author_facet	Yu Okuma Tomoaki Aoki Santiago J. Miyara Kei Hayashida Mitsuaki Nishikimi Ryosuke Takegawa Tai Yin Junhwan Kim Lance B. Becker Koichiro Shinozaki
author_sort	Yu Okuma
title	The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model
title_short	The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model
title_full	The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model
title_fullStr	The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model
title_full_unstemmed	The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model
title_sort	evaluation of pituitary damage associated with cardiac arrest: an experimental rodent model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7fc1625c4de34b6c93af84813e35c18c
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The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model

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