Characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development

Abstract Sarcomatoid hepatocellular carcinoma (SHC) is a rare type of HCC with significantly poorer survival than ordinary HCC. Little is known about the mechanism associated with SHC and its biomarkers and therapy. Here, we established a mouse liver cancer cell line and designated as Ymac-1. A sarc...

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Autores principales:	Chia-Hung Yen, Chih-Chung Lai, Chen-Chung Liao, Sheng-Fan Wang, Yi-Jen Liao, Chien-Yi Tung, Jung-Hsien Hung, Shiu-Feng Huang, Yi-Ming Arthur Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fc683ec0bfb40a7af09f8ebb59e314f
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spelling	oai:doaj.org-article:7fc683ec0bfb40a7af09f8ebb59e314f2021-12-02T15:04:53ZCharacterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development10.1038/s41598-017-03164-32045-2322https://doaj.org/article/7fc683ec0bfb40a7af09f8ebb59e314f2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03164-3https://doaj.org/toc/2045-2322Abstract Sarcomatoid hepatocellular carcinoma (SHC) is a rare type of HCC with significantly poorer survival than ordinary HCC. Little is known about the mechanism associated with SHC and its biomarkers and therapy. Here, we established a mouse liver cancer cell line and designated as Ymac-1. A sarcomatous appearance was observed in the allograft tumor arose from Ymac-1. Liver-secreted plasma proteins were found in Ymac-1 cultured supernatant by proteomics analysis. The positive staining of CK7, CK8, Vimentin and the suppressed expression of AFP indicated that Ymac-1 is a SHC cell line. Compared to its original tumor, an elevated level of EMT markers, N-cadherin and Vimentin, was found in Ymac-1. Ymac-1 displayed a higher migration rate and side population percentage than a mouse ordinary HCC cell line-Hepa1-6. Microarray analysis was performed to identify potential biomarkers/therapeutic targets for SHC. G6pd, a vital enzyme in pentose phosphate pathway, is highly expressed in Ymac-1. Depletion of G6pd in Ymac-1 reduced CD133 expression and sphere formation. Positive correlations between G6PD and CD133 were observed in human specimen. Higher expression of both G6PD and CD133 in tumor were associated with poor survival. In summary Ymac-1 can be a useful SHC cell model for novel biomarker and therapy development.Chia-Hung YenChih-Chung LaiChen-Chung LiaoSheng-Fan WangYi-Jen LiaoChien-Yi TungJung-Hsien HungShiu-Feng HuangYi-Ming Arthur ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chia-Hung Yen Chih-Chung Lai Chen-Chung Liao Sheng-Fan Wang Yi-Jen Liao Chien-Yi Tung Jung-Hsien Hung Shiu-Feng Huang Yi-Ming Arthur Chen Characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development
description	Abstract Sarcomatoid hepatocellular carcinoma (SHC) is a rare type of HCC with significantly poorer survival than ordinary HCC. Little is known about the mechanism associated with SHC and its biomarkers and therapy. Here, we established a mouse liver cancer cell line and designated as Ymac-1. A sarcomatous appearance was observed in the allograft tumor arose from Ymac-1. Liver-secreted plasma proteins were found in Ymac-1 cultured supernatant by proteomics analysis. The positive staining of CK7, CK8, Vimentin and the suppressed expression of AFP indicated that Ymac-1 is a SHC cell line. Compared to its original tumor, an elevated level of EMT markers, N-cadherin and Vimentin, was found in Ymac-1. Ymac-1 displayed a higher migration rate and side population percentage than a mouse ordinary HCC cell line-Hepa1-6. Microarray analysis was performed to identify potential biomarkers/therapeutic targets for SHC. G6pd, a vital enzyme in pentose phosphate pathway, is highly expressed in Ymac-1. Depletion of G6pd in Ymac-1 reduced CD133 expression and sphere formation. Positive correlations between G6PD and CD133 were observed in human specimen. Higher expression of both G6PD and CD133 in tumor were associated with poor survival. In summary Ymac-1 can be a useful SHC cell model for novel biomarker and therapy development.
format	article
author	Chia-Hung Yen Chih-Chung Lai Chen-Chung Liao Sheng-Fan Wang Yi-Jen Liao Chien-Yi Tung Jung-Hsien Hung Shiu-Feng Huang Yi-Ming Arthur Chen
author_facet	Chia-Hung Yen Chih-Chung Lai Chen-Chung Liao Sheng-Fan Wang Yi-Jen Liao Chien-Yi Tung Jung-Hsien Hung Shiu-Feng Huang Yi-Ming Arthur Chen
author_sort	Chia-Hung Yen
title	Characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development
title_short	Characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development
title_full	Characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development
title_fullStr	Characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development
title_full_unstemmed	Characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development
title_sort	characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7fc683ec0bfb40a7af09f8ebb59e314f
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Characterization of a new murine cell line of sarcomatoid hepatocellular carcinoma and its application for biomarker/therapy development

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