Proteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.

In Candida albicans, lipid rafts (also called detergent-resistant membranes, DRMs) are involved in many cellular processes and contain many important proteins. In our previous study, we demonstrated that Rta2p was required for calcineurin-mediated azole resistance and sphingoid long-chain base relea...

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Autores principales:	Lin Wang, Yu Jia, Ren-Jie Tang, Zheng Xu, Yong-Bing Cao, Xin-Ming Jia, Yuan-Ying Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fcaaabd49234cc593818a96c25f3fbf
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spelling	oai:doaj.org-article:7fcaaabd49234cc593818a96c25f3fbf2021-11-18T07:17:17ZProteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.1932-620310.1371/journal.pone.0037768https://doaj.org/article/7fcaaabd49234cc593818a96c25f3fbf2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22662216/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In Candida albicans, lipid rafts (also called detergent-resistant membranes, DRMs) are involved in many cellular processes and contain many important proteins. In our previous study, we demonstrated that Rta2p was required for calcineurin-mediated azole resistance and sphingoid long-chain base release in C. albicans. Here, we found that Rta2p was co-localized with raft-constituted ergosterol on the plasma membrane of C. albicans. Furthermore, this membrane expression pattern was totally disturbed by inhibitors of either ergosterol or sphingolipid synthesis. Biochemical fractionation of DRMs together with immunoblot uncovered that Rta2p, along with well-known DRM-associated proteins (Pma1p and Gas1p homologue), was associated with DRMs and their associations were blocked by inhibitors of either ergosterol or sphingolipid synthesis. Finally, we used the proteomic analysis together with immunoblot and identified that Rta2p was required for the association of 10 proteins with DRMs. These 5 proteins (Pma1p, Gas1p homologue, Erg11p, Pmt2p and Ali1p) have been reported to be DRM-associated and also that Erg11p is a well-known target of azoles in C. albicans. In conclusion, our results showed that Rta2p was predominantly localized in lipid rafts and was required for the association of certain membrane proteins with lipid rafts in C. albicans.Lin WangYu JiaRen-Jie TangZheng XuYong-Bing CaoXin-Ming JiaYuan-Ying JiangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37768 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lin Wang Yu Jia Ren-Jie Tang Zheng Xu Yong-Bing Cao Xin-Ming Jia Yuan-Ying Jiang Proteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.
description	In Candida albicans, lipid rafts (also called detergent-resistant membranes, DRMs) are involved in many cellular processes and contain many important proteins. In our previous study, we demonstrated that Rta2p was required for calcineurin-mediated azole resistance and sphingoid long-chain base release in C. albicans. Here, we found that Rta2p was co-localized with raft-constituted ergosterol on the plasma membrane of C. albicans. Furthermore, this membrane expression pattern was totally disturbed by inhibitors of either ergosterol or sphingolipid synthesis. Biochemical fractionation of DRMs together with immunoblot uncovered that Rta2p, along with well-known DRM-associated proteins (Pma1p and Gas1p homologue), was associated with DRMs and their associations were blocked by inhibitors of either ergosterol or sphingolipid synthesis. Finally, we used the proteomic analysis together with immunoblot and identified that Rta2p was required for the association of 10 proteins with DRMs. These 5 proteins (Pma1p, Gas1p homologue, Erg11p, Pmt2p and Ali1p) have been reported to be DRM-associated and also that Erg11p is a well-known target of azoles in C. albicans. In conclusion, our results showed that Rta2p was predominantly localized in lipid rafts and was required for the association of certain membrane proteins with lipid rafts in C. albicans.
format	article
author	Lin Wang Yu Jia Ren-Jie Tang Zheng Xu Yong-Bing Cao Xin-Ming Jia Yuan-Ying Jiang
author_facet	Lin Wang Yu Jia Ren-Jie Tang Zheng Xu Yong-Bing Cao Xin-Ming Jia Yuan-Ying Jiang
author_sort	Lin Wang
title	Proteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.
title_short	Proteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.
title_full	Proteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.
title_fullStr	Proteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.
title_full_unstemmed	Proteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.
title_sort	proteomic analysis of rta2p-dependent raft-association of detergent-resistant membranes in candida albicans.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/7fcaaabd49234cc593818a96c25f3fbf
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Proteomic analysis of Rta2p-dependent raft-association of detergent-resistant membranes in Candida albicans.

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