Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography

Abstract For the first time, the three-dimensional (3D) internal structure of naturally produced Didymosphenia geminata frustules were nondestructively visualized at sub-100 nm resolution. The well-optimized hierarchical structures of these natural organisms provide insight that is needed to design...

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Autores principales:	Izabela Zgłobicka, Qiong Li, Jürgen Gluch, Magdalena Płocińska, Teresa Noga, Romuald Dobosz, Robert Szoszkiewicz, Andrzej Witkowski, Ehrenfried Zschech, Krzysztof J. Kurzydłowski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fcdab46c0ff4e838595d77ce9464f2a
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spelling	oai:doaj.org-article:7fcdab46c0ff4e838595d77ce9464f2a2021-12-02T11:40:50ZVisualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography10.1038/s41598-017-08960-52045-2322https://doaj.org/article/7fcdab46c0ff4e838595d77ce9464f2a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08960-5https://doaj.org/toc/2045-2322Abstract For the first time, the three-dimensional (3D) internal structure of naturally produced Didymosphenia geminata frustules were nondestructively visualized at sub-100 nm resolution. The well-optimized hierarchical structures of these natural organisms provide insight that is needed to design novel, environmentally friendly functional materials. Diatoms, which are widely distributed in freshwater, seawater and wet soils, are well known for their intricate, siliceous cell walls called ‘frustules’. Each type of diatom has a specific morphology with various pores, ribs, minute spines, marginal ridges and elevations. In this paper, the visualization is performed using nondestructive nano X-ray computed tomography (nano-XCT). Arbitrary cross-sections through the frustules, which can be extracted from the nano-XCT 3D data set for each direction, are validated via the destructive focused ion beam (FIB) cross-sectioning of regions of interest (ROIs) and subsequent observation by scanning electron microscopy (SEM). These 3D data are essential for understanding the functionality and potential applications of diatom cells.Izabela ZgłobickaQiong LiJürgen GluchMagdalena PłocińskaTeresa NogaRomuald DoboszRobert SzoszkiewiczAndrzej WitkowskiEhrenfried ZschechKrzysztof J. KurzydłowskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Izabela Zgłobicka Qiong Li Jürgen Gluch Magdalena Płocińska Teresa Noga Romuald Dobosz Robert Szoszkiewicz Andrzej Witkowski Ehrenfried Zschech Krzysztof J. Kurzydłowski Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography
description	Abstract For the first time, the three-dimensional (3D) internal structure of naturally produced Didymosphenia geminata frustules were nondestructively visualized at sub-100 nm resolution. The well-optimized hierarchical structures of these natural organisms provide insight that is needed to design novel, environmentally friendly functional materials. Diatoms, which are widely distributed in freshwater, seawater and wet soils, are well known for their intricate, siliceous cell walls called ‘frustules’. Each type of diatom has a specific morphology with various pores, ribs, minute spines, marginal ridges and elevations. In this paper, the visualization is performed using nondestructive nano X-ray computed tomography (nano-XCT). Arbitrary cross-sections through the frustules, which can be extracted from the nano-XCT 3D data set for each direction, are validated via the destructive focused ion beam (FIB) cross-sectioning of regions of interest (ROIs) and subsequent observation by scanning electron microscopy (SEM). These 3D data are essential for understanding the functionality and potential applications of diatom cells.
format	article
author	Izabela Zgłobicka Qiong Li Jürgen Gluch Magdalena Płocińska Teresa Noga Romuald Dobosz Robert Szoszkiewicz Andrzej Witkowski Ehrenfried Zschech Krzysztof J. Kurzydłowski
author_facet	Izabela Zgłobicka Qiong Li Jürgen Gluch Magdalena Płocińska Teresa Noga Romuald Dobosz Robert Szoszkiewicz Andrzej Witkowski Ehrenfried Zschech Krzysztof J. Kurzydłowski
author_sort	Izabela Zgłobicka
title	Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography
title_short	Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography
title_full	Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography
title_fullStr	Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography
title_full_unstemmed	Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography
title_sort	visualization of the internal structure of didymosphenia geminata frustules using nano x-ray tomography
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7fcdab46c0ff4e838595d77ce9464f2a
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Visualization of the internal structure of Didymosphenia geminata frustules using nano X-ray tomography

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