Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system

Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities...

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Autores principales:	Zainul Amiruddin Zakaria, Nur Diyana Mahmood, Maizatul Hasyima Omar, Muhammad Taher, Rusliza Basir
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	muntingiaceae hepatoprotective activity antioxidant activity oxidative stress markers pro-inflammatory mediators phytoconstituents uhplc-esi gcms Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/7fd0fbd37089463eace52936abac75b9
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spelling	oai:doaj.org-article:7fd0fbd37089463eace52936abac75b92021-11-17T14:21:56ZMethanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system1388-02091744-511610.1080/13880209.2019.1606836https://doaj.org/article/7fd0fbd37089463eace52936abac75b92019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1606836https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities in CCl4-induced hepatotoxic rat. Materials and methods: Sprague Dawley rats (n = 6) were treated (p.o.) with 10% DMSO (Groups 1 and 2), 50 mg/kg N-acetylcysteine (Group 3) or, 50, 250, or 500 mg/kg MMCL (Groups 4–6) for 7 consecutive days followed by pretreatment (i.p.) with vehicle (Group 1) or 50% CCl4 in olive oil (v/v) (Groups 2–6) on day 7th. Plasma liver enzymes and hepatic antioxidant enzymes and pro-inflammatory cytokines concentrations were measured while liver histopathology was examined. Results: MMCL, at 500 mg/kg, significantly (p < 0.05) ameliorated CCl4-induced hepatotoxicity by decreasing the plasma level of alanine transaminase (429.1 versus 168.7 U/L) and aspartate transaminase (513.8 versus 438.1 U/L) as well as the tissue level of nitric oxide (62.7 versus 24.1 nmol/g tissue). At 50, 250, or 500 mg/kg, MMCL significantly (p < 0.05) reduced the tumour necrosis factor α (87.8 versus 32.7 pg/mg tissue), interleukin-1β (1474.4 versus 618.3 pg/mg tissue), and interleukin-6 (136.7 versus 30.8 pg/mg tissue) while increased the liver catalase (92.1 versus 114.4 U/g tissue) and superoxide dismutase (3.4 versus 5.5 U/g tissue). Additionally, qualitative phytochemicals analysis showed that MMCL contained gallic acid, ferulic acid, quercetin, and genistein. Discussion and conclusions: MMCL ability to attenuate CCl4-induced hepatotoxicity could be helpful in the development of hepatoprotective agents with fewer side effects.Zainul Amiruddin ZakariaNur Diyana MahmoodMaizatul Hasyima OmarMuhammad TaherRusliza BasirTaylor & Francis Grouparticlemuntingiaceaehepatoprotective activityantioxidant activityoxidative stress markerspro-inflammatory mediatorsphytoconstituentsuhplc-esigcmsTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 335-344 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	muntingiaceae hepatoprotective activity antioxidant activity oxidative stress markers pro-inflammatory mediators phytoconstituents uhplc-esi gcms Therapeutics. Pharmacology RM1-950
spellingShingle	muntingiaceae hepatoprotective activity antioxidant activity oxidative stress markers pro-inflammatory mediators phytoconstituents uhplc-esi gcms Therapeutics. Pharmacology RM1-950 Zainul Amiruddin Zakaria Nur Diyana Mahmood Maizatul Hasyima Omar Muhammad Taher Rusliza Basir Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
description	Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities in CCl4-induced hepatotoxic rat. Materials and methods: Sprague Dawley rats (n = 6) were treated (p.o.) with 10% DMSO (Groups 1 and 2), 50 mg/kg N-acetylcysteine (Group 3) or, 50, 250, or 500 mg/kg MMCL (Groups 4–6) for 7 consecutive days followed by pretreatment (i.p.) with vehicle (Group 1) or 50% CCl4 in olive oil (v/v) (Groups 2–6) on day 7th. Plasma liver enzymes and hepatic antioxidant enzymes and pro-inflammatory cytokines concentrations were measured while liver histopathology was examined. Results: MMCL, at 500 mg/kg, significantly (p < 0.05) ameliorated CCl4-induced hepatotoxicity by decreasing the plasma level of alanine transaminase (429.1 versus 168.7 U/L) and aspartate transaminase (513.8 versus 438.1 U/L) as well as the tissue level of nitric oxide (62.7 versus 24.1 nmol/g tissue). At 50, 250, or 500 mg/kg, MMCL significantly (p < 0.05) reduced the tumour necrosis factor α (87.8 versus 32.7 pg/mg tissue), interleukin-1β (1474.4 versus 618.3 pg/mg tissue), and interleukin-6 (136.7 versus 30.8 pg/mg tissue) while increased the liver catalase (92.1 versus 114.4 U/g tissue) and superoxide dismutase (3.4 versus 5.5 U/g tissue). Additionally, qualitative phytochemicals analysis showed that MMCL contained gallic acid, ferulic acid, quercetin, and genistein. Discussion and conclusions: MMCL ability to attenuate CCl4-induced hepatotoxicity could be helpful in the development of hepatoprotective agents with fewer side effects.
format	article
author	Zainul Amiruddin Zakaria Nur Diyana Mahmood Maizatul Hasyima Omar Muhammad Taher Rusliza Basir
author_facet	Zainul Amiruddin Zakaria Nur Diyana Mahmood Maizatul Hasyima Omar Muhammad Taher Rusliza Basir
author_sort	Zainul Amiruddin Zakaria
title	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_short	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_full	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_fullStr	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_full_unstemmed	Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
title_sort	methanol extract of muntingia calabura leaves attenuates ccl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/7fd0fbd37089463eace52936abac75b9
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Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system

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