Preventive effects of Lactobacillus johnsonii on the renal injury of mice induced by high fluoride exposure: Insights from colonic microbiota and co-occurrence network analysis
Fluoride (F) exposure was widely reported to be associated with renal diseases. Since absorbed F enters the organism from drinking water mostly through the gastrointestinal tract, investigating changes of gut microbes may have profound implications for the prevention of chronic F exposure because in...
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Elsevier
2021
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oai:doaj.org-article:7fd218dbfee04c6e8607abf025f114f62021-11-26T04:23:14ZPreventive effects of Lactobacillus johnsonii on the renal injury of mice induced by high fluoride exposure: Insights from colonic microbiota and co-occurrence network analysis0147-651310.1016/j.ecoenv.2021.113006https://doaj.org/article/7fd218dbfee04c6e8607abf025f114f62021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321011180https://doaj.org/toc/0147-6513Fluoride (F) exposure was widely reported to be associated with renal diseases. Since absorbed F enters the organism from drinking water mostly through the gastrointestinal tract, investigating changes of gut microbes may have profound implications for the prevention of chronic F exposure because increasing evidence supported the existence of the gut-kidney axis. In the present study, we aimed to explore the potential positive effects of probiotics on high F exposure-induced renal lesions and dysfunction in mice by the modulation of the colonic microbiota. Mice were fed with normal (Ctrl group) or sodium-fluoride (F and Prob groups; 100 mg/L sodium fluoride (NaF)) drinking water with or without Lactobaillus johnsonii BS15, a probiotic strain proven to be preventive for F exposure. Mice fed with sodium-fluoride drinking water alone exhibited renal tissue damages, decreased the renal antioxidant capability and dysfunction. In contrast, L. johnsonii BS15 reversed these F-induced renal changes. 16S rRNA gene sequencing shows that L. johnsonii BS15 alleviated the increased community diversity (Shannon diversity) and richness index (number of observed features) as well as the distured structure of colon microbiota in F-exposed mice. A total of 13 OTUs with increased relative abundance were identified as the keystone OTUs in F-exposed mice based on the analysis of degree of co-occurrence and abundance of OTUs. Moreover, Spearman’s rank correlation shows that the 13 keystone OTUs had negative effect on renal health and intestinal integrity. L. johnsonii BS15 reversed four of keystone OTUs (OTU 5, OTU 794, OTU 1035, and OTU 868) changes which might be related to the underlying protected mechanism of L. johnsonii BS15 against F-induced renal damages.Jinge XinNing SunHesong WangHailin MaBangyuan WuLianxin LiYanyan WangHaonan HuangDong ZengXiuquan BaiAli ChenShenhai GongXueqin NiYang BaiElsevierarticle16S rRNA sequencingFluoride-induced damagesGut microbotaProbioticsRenal dysfunctionEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 113006- (2021) |
| institution |
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| collection |
DOAJ |
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EN |
| topic |
16S rRNA sequencing Fluoride-induced damages Gut microbota Probiotics Renal dysfunction Environmental pollution TD172-193.5 Environmental sciences GE1-350 |
| spellingShingle |
16S rRNA sequencing Fluoride-induced damages Gut microbota Probiotics Renal dysfunction Environmental pollution TD172-193.5 Environmental sciences GE1-350 Jinge Xin Ning Sun Hesong Wang Hailin Ma Bangyuan Wu Lianxin Li Yanyan Wang Haonan Huang Dong Zeng Xiuquan Bai Ali Chen Shenhai Gong Xueqin Ni Yang Bai Preventive effects of Lactobacillus johnsonii on the renal injury of mice induced by high fluoride exposure: Insights from colonic microbiota and co-occurrence network analysis |
| description |
Fluoride (F) exposure was widely reported to be associated with renal diseases. Since absorbed F enters the organism from drinking water mostly through the gastrointestinal tract, investigating changes of gut microbes may have profound implications for the prevention of chronic F exposure because increasing evidence supported the existence of the gut-kidney axis. In the present study, we aimed to explore the potential positive effects of probiotics on high F exposure-induced renal lesions and dysfunction in mice by the modulation of the colonic microbiota. Mice were fed with normal (Ctrl group) or sodium-fluoride (F and Prob groups; 100 mg/L sodium fluoride (NaF)) drinking water with or without Lactobaillus johnsonii BS15, a probiotic strain proven to be preventive for F exposure. Mice fed with sodium-fluoride drinking water alone exhibited renal tissue damages, decreased the renal antioxidant capability and dysfunction. In contrast, L. johnsonii BS15 reversed these F-induced renal changes. 16S rRNA gene sequencing shows that L. johnsonii BS15 alleviated the increased community diversity (Shannon diversity) and richness index (number of observed features) as well as the distured structure of colon microbiota in F-exposed mice. A total of 13 OTUs with increased relative abundance were identified as the keystone OTUs in F-exposed mice based on the analysis of degree of co-occurrence and abundance of OTUs. Moreover, Spearman’s rank correlation shows that the 13 keystone OTUs had negative effect on renal health and intestinal integrity. L. johnsonii BS15 reversed four of keystone OTUs (OTU 5, OTU 794, OTU 1035, and OTU 868) changes which might be related to the underlying protected mechanism of L. johnsonii BS15 against F-induced renal damages. |
| format |
article |
| author |
Jinge Xin Ning Sun Hesong Wang Hailin Ma Bangyuan Wu Lianxin Li Yanyan Wang Haonan Huang Dong Zeng Xiuquan Bai Ali Chen Shenhai Gong Xueqin Ni Yang Bai |
| author_facet |
Jinge Xin Ning Sun Hesong Wang Hailin Ma Bangyuan Wu Lianxin Li Yanyan Wang Haonan Huang Dong Zeng Xiuquan Bai Ali Chen Shenhai Gong Xueqin Ni Yang Bai |
| author_sort |
Jinge Xin |
| title |
Preventive effects of Lactobacillus johnsonii on the renal injury of mice induced by high fluoride exposure: Insights from colonic microbiota and co-occurrence network analysis |
| title_short |
Preventive effects of Lactobacillus johnsonii on the renal injury of mice induced by high fluoride exposure: Insights from colonic microbiota and co-occurrence network analysis |
| title_full |
Preventive effects of Lactobacillus johnsonii on the renal injury of mice induced by high fluoride exposure: Insights from colonic microbiota and co-occurrence network analysis |
| title_fullStr |
Preventive effects of Lactobacillus johnsonii on the renal injury of mice induced by high fluoride exposure: Insights from colonic microbiota and co-occurrence network analysis |
| title_full_unstemmed |
Preventive effects of Lactobacillus johnsonii on the renal injury of mice induced by high fluoride exposure: Insights from colonic microbiota and co-occurrence network analysis |
| title_sort |
preventive effects of lactobacillus johnsonii on the renal injury of mice induced by high fluoride exposure: insights from colonic microbiota and co-occurrence network analysis |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/7fd218dbfee04c6e8607abf025f114f6 |
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