The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis

Abstract Various disease-related genes have recently been identified using single nucleotide polymorphisms (SNPs). This study identified disease-related genes by analyzing SNP using genomic DNA isolated from Japanese patients with periapical periodontitis. Results showed that the SNP in LRP5 demonst...

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Autores principales:	Haruna Naruse, Shousaku Itoh, Yuki Itoh, Takumi Kagioka, Makoto Abe, Mikako Hayashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7fdb333cea45438a86894a5eb1dcca21
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spelling	oai:doaj.org-article:7fdb333cea45438a86894a5eb1dcca212021-12-02T16:57:08ZThe Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis10.1038/s41598-021-99231-x2045-2322https://doaj.org/article/7fdb333cea45438a86894a5eb1dcca212021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-99231-xhttps://doaj.org/toc/2045-2322Abstract Various disease-related genes have recently been identified using single nucleotide polymorphisms (SNPs). This study identified disease-related genes by analyzing SNP using genomic DNA isolated from Japanese patients with periapical periodontitis. Results showed that the SNP in LRP5 demonstrated a significant genotypic association with periapical lesions (Fisher’s exact test, P < 0.05). We constructed an in vivo murine periapical periodontitis model to confirm the Wnt/β-catenin signaling pathway’s role in developing and healing periapical periodontitis. We observed that administration of the Wnt/β-catenin signaling pathway inhibitor enlarged the periapical lesion. Moreover, applying lithium chloride (LiCl) to root canals accelerated periapical periodontitis healing. Histological analysis demonstrated that the expression levels of Col1a1 and Runx2 increased in the LiCl application group compared to that in the control group. Furthermore, many CD45R-positive cells appeared in the periapical lesions in the LiCl application group. These results indicated that LiCl promoted the healing of periapical periodontitis by inducing bone formation and immune responses. Our findings suggest that the Wnt/β-catenin signaling pathway regulates the development of periapical periodontitis. We propose a bioactive next-generation root canal treatment agent for this dental lesion.Haruna NaruseShousaku ItohYuki ItohTakumi KagiokaMakoto AbeMikako HayashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haruna Naruse Shousaku Itoh Yuki Itoh Takumi Kagioka Makoto Abe Mikako Hayashi The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis
description	Abstract Various disease-related genes have recently been identified using single nucleotide polymorphisms (SNPs). This study identified disease-related genes by analyzing SNP using genomic DNA isolated from Japanese patients with periapical periodontitis. Results showed that the SNP in LRP5 demonstrated a significant genotypic association with periapical lesions (Fisher’s exact test, P < 0.05). We constructed an in vivo murine periapical periodontitis model to confirm the Wnt/β-catenin signaling pathway’s role in developing and healing periapical periodontitis. We observed that administration of the Wnt/β-catenin signaling pathway inhibitor enlarged the periapical lesion. Moreover, applying lithium chloride (LiCl) to root canals accelerated periapical periodontitis healing. Histological analysis demonstrated that the expression levels of Col1a1 and Runx2 increased in the LiCl application group compared to that in the control group. Furthermore, many CD45R-positive cells appeared in the periapical lesions in the LiCl application group. These results indicated that LiCl promoted the healing of periapical periodontitis by inducing bone formation and immune responses. Our findings suggest that the Wnt/β-catenin signaling pathway regulates the development of periapical periodontitis. We propose a bioactive next-generation root canal treatment agent for this dental lesion.
format	article
author	Haruna Naruse Shousaku Itoh Yuki Itoh Takumi Kagioka Makoto Abe Mikako Hayashi
author_facet	Haruna Naruse Shousaku Itoh Yuki Itoh Takumi Kagioka Makoto Abe Mikako Hayashi
author_sort	Haruna Naruse
title	The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis
title_short	The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis
title_full	The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis
title_fullStr	The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis
title_full_unstemmed	The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis
title_sort	wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7fdb333cea45438a86894a5eb1dcca21
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The Wnt/β-catenin signaling pathway has a healing ability for periapical periodontitis

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